##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
Acc65I AccIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI Asp718I
AspEI AspI AviII AvrII BbvCI
BcgI BlnI BsaBI BseAI BsiEI
BsiWI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII Bsu36I Cfr10I
ClaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRV EcoT22I FseI
FspI HpaI KpnI KspI MamI
MluI MroI NaeI NdeI NgoMIV
NotI NruI NsiI PacI PflFI
PinAI PmeI PvuI RcaI SacII
SalI SexAI SfiI SgrAI SmaI
SnaBI SphI SspI SwaI Tth111I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 186 GACGTC GACGT^C
AccII 101 CGCG CG^CG
AclI 3173 AACGTT AA^CGTT
BbrPI 3705 CACGTG CAC^GTG
BsaI 4276 GAGACC GGTCTC(1/5)
BsaWI 5855 ACCGGA [AT]^CCGG[AT]
Bsp1407I 2660 TGTACA T^GTACA
BsrGI 2660 TGTACA T^GTACA
Bst1107I 2162 GTATAC GTA^TAC
BstBI 4366 TTCGAA TT^CGAA
BstEII 5396 GGTCACC G^GT[ACGT]ACC
BstPI 5396 GGTCACC G^GT[ACGT]ACC
BstUI 101 CGCG CG^CG
BstZ17I 2162 GTATAC GTA^TAC
CpoI 3719 CGGTCCG CG^G[AT]CCG
DraI 3093 TTTAAA TTT^AAA
EcoO65I 5396 GGTCACC G^GT[ACGT]ACC
EcoRI 20 GAATTC G^AATTC
HincII 3973 GTCAAC GT[CT]^[GA]AC
HindII 3973 GTCAAC GT[CT]^[GA]AC
MfeI 5499 CAATTG C^AATTG
MslI 2613 CACCTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 5499 CAATTG C^AATTG
MvnI 101 CGCG CG^CG
NheI 2274 GCTAGC G^CTAGC
NspV 4366 TTCGAA TT^CGAA
PaeR7I 5815 CTCGAG C^TCGAG
PmaCI 3705 CACGTG CAC^GTG
PmlI 3705 CACGTG CAC^GTG
PshAI 2242 GACTTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3173 AACGTT AA^CGTT
RsrII 3719 CGGTCCG CG^G[AT]CCG
SbfI 1898 CCTGCAGG CCTGCA^GG
ScaI 5911 AGTACT AGT^ACT
SfuI 4366 TTCGAA TT^CGAA
SpeI 111 ACTAGT A^CTAGT
Sse8387I 1898 CCTGCAGG CCTGCA^GG
SspBI 2660 TGTACA T^GTACA
XhoI 5815 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 2162 GTATAC GT^[AC][GT]AC
2249 GTCTAC
AflII 2118 CTTAAG C^TTAAG
2405 CTTAAG
AflIII 1630 ACGTGT A^C[GA][CT]GT
3704 ACACGT
ApaI 2443 GGGCCC GGGCC^C
3616 GGGCCC
AseI 2322 ATTAAT AT^TAAT
3468 ATTAAT
AsnI 2322 ATTAAT AT^TAAT
3468 ATTAAT
AvaI 5340 CCCGAG C^[CT]CG[GA]G
5815 CTCGAG
BamHI 1033 GGATCC G^GATCC
4566 GGATCC
BbeI 30 GGCGCC GGCGC^C
117 GGCGCC
BciVI 2400 GGATAC GTATCC(6/5)
156 GTATCC
BcnI 85 CCCGG CC^[GC]GG
2995 CCCGG
BfrI 2118 CTTAAG C^TTAAG
2405 CTTAAG
BsaAI 3696 TACGTG [CT]AC^GT[GA]
3705 CACGTG
BsmBI 3018 CGTCTC CGTCTC(1/5)
717 GAGACG
BsoBI 5340 CCCGAG C^[CT]CG[GA]G
5815 CTCGAG
BssSI 777 CACGAG CACGAG(-5/-1)
4273 CACGAG
DrdI 186 GACGTCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3449 GACTGATCAGTC
KasI 30 GGCGCC G^GCGCC
117 GGCGCC
NarI 30 GGCGCC GG^CGCC
117 GGCGCC
NciI 85 CCCGG CC^[GC]GG
2995 CCCGG
PshBI 2322 ATTAAT AT^TAAT
3468 ATTAAT
SfoI 30 GGCGCC GGC^GCC
117 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BalI BanI BanII BbsI
BbvI BclI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI