##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspHI BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII Hin1I
HindIII KasI KspI MamI MfeI
MluI MroI MunI NarI NotI
NsiI NspV PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SwaI
Tth111I Van91I XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 760 GGTACC G^GTACC
AccII 2414 CGCG CG^CG
AflIII 2388 ACATGT A^C[GA][CT]GT
AocI 690 CCTGAGG CC^T[ACGT]AGG
Asp718I 760 GGTACC G^GTACC
BbvCI 1007 CCTCAGC CCTCAGC(-5/-2)
BciVI 1334 GGATAC GTATCC(6/5)
BglII 2102 AGATCT A^GATCT
BsaWI 1726 TCCGGT [AT]^CCGG[AT]
BsmBI 269 GAGACG CGTCTC(1/5)
BspLU11I 2388 ACATGT A^CATGT
BsrFI 1544 GCCGGC [GA]^CCGG[CT]
BssSI 193 CTCGTG CACGAG(-5/-1)
BstAPI 1879 GCAACTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2414 CGCG CG^CG
Bsu36I 690 CCTGAGG CC^T[ACGT]AGG
CfoI 1534 GCGC GCG^C
Cfr10I 1544 GCCGGC [GA]^CCGG[CT]
Eco81I 690 CCTGAGG CC^T[ACGT]AGG
HgaI 271 GACGC GACGC(5/10)
HhaI 1534 GCGC GCG^C
HinP1I 1534 GCGC G^CGC
HpaI 2180 GTTAAC GTT^AAC
KpnI 760 GGTACC GGTAC^C
MspA1I 2577 CAGCTG C[AC]G^C[GT]G
MvnI 2414 CGCG CG^CG
NaeI 1544 GCCGGC GCC^GGC
NdeI 1949 CATATG CA^TATG
NgoMIV 1544 GCCGGC G^CCGGC
NruI 2413 TCGCGA TCG^CGA
PacI 2734 TTAATTAA TTAAT^TAA
PaeR7I 2395 CTCGAG C^TCGAG
PstI 666 CTGCAG CTGCA^G
PvuII 2577 CAGCTG CAG^CTG
SacI 1861 GAGCTC GAGCT^C
SapI 234 GAAGAGC GCTCTTC(1/4)
SbfI 665 CCTGCAGG CCTGCA^GG
SexAI 1369 ACCTGGT A^CC[AT]GGT
SfcI 666 CTGCAG C^T[GA][CT]AG
SfiI 168 GGCCCTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 665 CCTGCAGG CCTGCA^GG
SspI 250 AATATT AAT^ATT
StuI 1278 AGGCCT AGG^CCT
XbaI 2316 TCTAGA T^CTAGA
XhoI 2395 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BbsI 899 GAAGAC GAAGAC(2/6)
1286 GTCTTC
BcgI 527 GCAAGAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1053 GCATCCCCCTCG
BcnI 605 CCGGG CC^[GC]GG
1682 CCGGG
BpuAI 899 GAAGAC GAAGAC(2/6)
1286 GTCTTC
BsaI 10 GAGACC GGTCTC(1/5)
364 GAGACC
BsgI 497 CTGCAC GTGCAG(16/14)
967 CTGCAC
Bsp1407I 61 TGTACA T^GTACA
1319 TGTACA
BsrGI 61 TGTACA T^GTACA
1319 TGTACA
EcoNI 1212 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2545 CCTCTAGCAGG
FauI 524 CCCGC CCCGC(4/6)
862 CCCGC
MaeII 215 ACGT A^CGT
617 ACGT
NciI 605 CCGGG CC^[GC]GG
1682 CCGGG
NheI 1257 GCTAGC G^CTAGC
1764 GCTAGC
NspI 541 ACATGC [GA]CATG^[CT]
2388 ACATGT
PpuMI 2541 AGGTCCT [GA]G^G[AT]CC[CT]
2625 AGGTCCC
SspBI 61 TGTACA T^GTACA
1319 TGTACA
TaqI 1551 TCGA T^CGA
2396 TCGA
TthHB8I 1551 TCGA T^CGA
2396 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI AspHI AvaI AvaII
BalI BanI BanII BbvI BfaI
BglI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII