##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BlnI BsaAI BsaBI BseAI BseRI
BsiEI BsiWI BsmBI BspDI BspEI
BspLU11I BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CfoI Cfr10I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoT22I
FbaI FseI FspI HaeII HhaI
HinP1I KasI KpnI KspI MamI
MluI MroI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 2580 GACGTC GACGT^C
AccI 1052 GTCTAC GT^[AC][GT]AC
AccII 1359 CGCG CG^CG
AcyI 2580 GACGTC G[GA]^CG[CT]C
Alw44I 2963 GTGCAC G^TGCAC
AocI 2254 CCTAAGG CC^T[ACGT]AGG
ApaLI 2963 GTGCAC G^TGCAC
AspI 1839 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3018 CCCGGG C^[CT]CG[GA]G
BbvCI 2859 GCTGAGG CCTCAGC(-5/-2)
BlpI 1957 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1957 GCTCAGC GC^T[ACGT]AGC
BsaHI 2580 GACGTC G[GA]^CG[CT]C
BsaWI 2363 ACCGGA [AT]^CCGG[AT]
BsmI 2367 GAATGC GAATGC(1/-1)
BsoBI 3018 CCCGGG C^[CT]CG[GA]G
BspHI 2450 TCATGA T^CATGA
BspMI 853 ACCTGC ACCTGC(4/8)
BsrBI 1629 CCGCTC CCGCTC(-3/-3)
BssSI 2108 CACGAG CACGAG(-5/-1)
BstAPI 3026 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1359 CGCG CG^CG
BstYI 1685 AGATCC [GA]^GATC[CT]
Bsu36I 2254 CCTAAGG CC^T[ACGT]AGG
CelII 1957 GCTCAGC GC^T[ACGT]AGC
DraIII 863 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 900 CCATGG C^C[GA][CT]GG
Eco81I 2254 CCTAAGG CC^T[ACGT]AGG
EcoRI 1354 GAATTC G^AATTC
EcoRV 2063 GATATC GAT^ATC
FauI 1360 GCGGG CCCGC(4/6)
HgaI 634 GACGC GACGC(5/10)
Hin1I 2580 GACGTC G[GA]^CG[CT]C
HpaI 1489 GTTAAC GTT^AAC
MfeI 2171 CAATTG C^AATTG
MflI 1685 AGATCC [GA]^GATC[CT]
MunI 2171 CAATTG C^AATTG
MvnI 1359 CGCG CG^CG
NcoI 900 CCATGG C^CATGG
PflFI 1839 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 393 GACTC GAGTC(4/5)
PpuMI 1517 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1474 GACATACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2450 TCATGA T^CATGA
SapI 2341 GAAGAGC GCTCTTC(1/4)
SmaI 3018 CCCGGG CCC^GGG
Tth111I 1839 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1685 AGATCC [GA]^GATC[CT]
XmaCI 3018 CCCGGG C^CCGGG
XmaI 3018 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AhdI 533 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1894 GACTATGGGTC
AlwI 1686 GATCC GGATC(4/5)
1794 GGATC
AspEI 533 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1894 GACTATGGGTC
AvaII 575 GGACC G^G[AT]CC
1518 GGTCC
BanII 18 GGGCTC G[GA]GC[CT]^C
2513 GGGCTC
BcnI 3018 CCCGG CC^[GC]GG
3019 CCGGG
BfaI 449 CTAG C^TAG
2473 CTAG
Bsp1407I 740 TGTACA T^GTACA
2282 TGTACA
BsrGI 740 TGTACA T^GTACA
2282 TGTACA
DraII 930 GGGGCCT [GA]G^G[ACGT]CC[CT]
1517 GGGTCCT
Eam1105I 533 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1894 GACTATGGGTC
EarI 2383 CTCTTC CTCTTC(1/4)
2341 GAAGAG
EcoNI 121 CCTGTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1200 CCTGGCTCAGG
EcoO109I 930 GGGGCCT [GA]G^G[ACGT]CC[CT]
1517 GGGTCCT
HapII 2364 CCGG C^CGG
3019 CCGG
HincII 1489 GTTAAC GT[CT]^[GA]AC
2583 GTCAAC
HindII 1489 GTTAAC GT[CT]^[GA]AC
2583 GTCAAC
HpaII 2364 CCGG C^CGG
3019 CCGG
Ksp632I 2383 CTCTTC CTCTTC(1/4)
2341 GAAGAG
MaeI 449 CTAG C^TAG
2473 CTAG
MspA1I 1227 CAGCTG C[AC]G^C[GT]G
2215 CAGCTG
MspI 2364 CCGG C^CGG
3019 CCGG
NciI 3018 CCCGG CC^[GC]GG
3019 CCGGG
NspI 2249 GCATGC [GA]CATG^[CT]
2519 GCATGC
PflMI 1842 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2226 CCATCTCCTGG
PstI 66 CTGCAG CTGCA^G
2351 CTGCAG
PvuII 1227 CAGCTG CAG^CTG
2215 CAGCTG
SpeI 448 ACTAGT A^CTAGT
2472 ACTAGT
SphI 2249 GCATGC GCATG^C
2519 GCATGC
SspBI 740 TGTACA T^GTACA
2282 TGTACA
SspI 1825 AATATT AAT^ATT
2102 AATATT
StuI 2610 AGGCCT AGG^CCT
2653 AGGCCT
Van91I 1842 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2226 CCATCTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI BalI BbsI BbvI
BmyI BpmI BpuAI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstXI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HindIII HinfI
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI