##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AclI AflII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI AspEI
AviII AvrII BamHI BbeI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaI BsiEI BsiWI BspDI BspHI
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KasI MluI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI StuI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 211 GACGTC GACGT^C
Acc65I 3708 GGTACC G^GTACC
Aor51HI 1266 AGCGCT AGC^GCT
ApaI 3130 GGGCCC GGGCC^C
Asp718I 3708 GGTACC G^GTACC
AspI 814 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2339 CACGTG CAC^GTG
BbvCI 3391 GCTGAGG CCTCAGC(-5/-2)
BciVI 2590 GTATCC GTATCC(6/5)
BsaBI 529 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2530 GCATTC GAATGC(1/-1)
BspLU11I 1017 ACATGT A^CATGT
BsrBI 2673 GAGCGG CCGCTC(-3/-3)
BssHII 3750 GCGCGC G^CGCGC
BssSI 1404 CACGAG CACGAG(-5/-1)
BstXI 3241 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 848 GGATCT [GA]^GATC[CT]
DraIII 839 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1266 AGCGCT AGC^GCT
KpnI 3708 GGTACC GGTAC^C
KspI 2008 CCGCGG CCGC^GG
MamI 529 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2561 CAATTG C^AATTG
MflI 848 GGATCT [GA]^GATC[CT]
MunI 2561 CAATTG C^AATTG
NcoI 636 CCATGG C^CATGG
PflFI 814 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2339 CACGTG CAC^GTG
PmlI 2339 CACGTG CAC^GTG
PpuMI 1344 AGGTCCT [GA]G^G[AT]CC[CT]
SacII 2008 CCGCGG CCGC^GG
SalI 482 GTCGAC G^TCGAC
SapI 3039 GCTCTTC GCTCTTC(1/4)
SwaI 1531 ATTTAAAT ATTT^AAAT
Tth111I 814 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3416 CCAAGAAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 848 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 482 GTCGAC GT^[AC][GT]AC
2703 GTAGAC
AccIII 525 TCCGGA T^CCGGA
1101 TCCGGA
AflIII 1017 ACATGT A^C[GA][CT]GT
2338 ACACGT
AocI 1815 CCTGAGG CC^T[ACGT]AGG
3274 CCTAAGG
Asp700I 2526 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3586 GAAGCATTTC
BbsI 4144 GAAGAC GAAGAC(2/6)
1081 GTCTTC
BcgI 593 GCAGAAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3397 GCAGGAGAATCG
BglI 1041 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2033 GCCGGGAAGGC
BpuAI 4144 GAAGAC GAAGAC(2/6)
1081 GTCTTC
BsaAI 2339 CACGTG [CT]AC^GT[GA]
2601 CACGTA
BsaWI 525 TCCGGA [AT]^CCGG[AT]
1101 TCCGGA
BseAI 525 TCCGGA T^CCGGA
1101 TCCGGA
Bsp1407I 1604 TGTACA T^GTACA
2335 TGTACA
BspEI 525 TCCGGA T^CCGGA
1101 TCCGGA
BsrDI 1978 GCAATG GCAATG(2/0)
3969 GCAATG
BsrGI 1604 TGTACA T^GTACA
2335 TGTACA
BstAPI 181 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3073 GCAACCTGTGC
Bsu36I 1815 CCTGAGG CC^T[ACGT]AGG
3274 CCTAAGG
DraI 778 TTTAAA TTT^AAA
1532 TTTAAA
Eco81I 1815 CCTGAGG CC^T[ACGT]AGG
3274 CCTAAGG
EcoNI 1246 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1993 CCTGCTTCAGG
MroI 525 TCCGGA T^CCGGA
1101 TCCGGA
NaeI 1451 GCCGGC GCC^GGC
3085 GCCGGC
NgoMIV 1451 GCCGGC G^CCGGC
3085 GCCGGC
NspI 346 ACATGC [GA]CATG^[CT]
1017 ACATGT
PflMI 1877 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3524 CCATACACTGG
PstI 179 CTGCAG CTGCA^G
2486 CTGCAG
SmaI 498 CCCGGG CCC^GGG
2547 CCCGGG
SmlI 1247 CTCAAG C^T[CT][GA]AG
4040 CTTGAG
SspBI 1604 TGTACA T^GTACA
2335 TGTACA
Van91I 1877 CCAAGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3524 CCATACACTGG
XmaCI 498 CCCGGG C^CCGGG
2547 CCCGGG
XmaI 498 CCCGGG C^CCGGG
2547 CCCGGG
XmnI 2526 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3586 GAAGCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I