##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BciVI BclI
BfrI BglII BlnI BsaBI BsaHI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRV FbaI FseI FspI
HaeII HgaI Hin1I KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NotI NruI PacI PflFI PmeI
PshBI PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SnaBI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 2230 GTCTAC GT^[AC][GT]AC
AccII 47 CGCG CG^CG
AclI 1472 AACGTT AA^CGTT
AflIII 765 ACATGT A^C[GA][CT]GT
AgeI 1017 ACCGGT A^CCGGT
ApaI 921 GGGCCC GGGCC^C
Asp700I 2869 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 3091 TGGCCA TGG^CCA
BamHI 114 GGATCC G^GATCC
BbrPI 3087 CACGTG CAC^GTG
BbsI 2869 GAAGAC GAAGAC(2/6)
BbvCI 1546 CCTCAGC CCTCAGC(-5/-2)
BcgI 2847 GCAGACTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1746 GCCAGGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 2869 GAAGAC GAAGAC(2/6)
BsaAI 3087 CACGTG [CT]AC^GT[GA]
BsaWI 1017 ACCGGT [AT]^CCGG[AT]
BsmBI 1928 CGTCTC CGTCTC(1/5)
BspLU11I 765 ACATGT A^CATGT
BsrBI 1769 CCGCTC CCGCTC(-3/-3)
BsrFI 1017 ACCGGT [GA]^CCGG[CT]
BstEII 3115 GGTGACC G^GT[ACGT]ACC
BstPI 3115 GGTGACC G^GT[ACGT]ACC
BstUI 47 CGCG CG^CG
BstYI 114 GGATCC [GA]^GATC[CT]
Cfr10I 1017 ACCGGT [GA]^CCGG[CT]
DraI 2802 TTTAAA TTT^AAA
DraIII 2483 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 3091 TGGCCA [CT]^GGCC[GA]
EcoNI 2128 CCTTTGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3115 GGTGACC G^GT[ACGT]ACC
EcoT22I 711 ATGCAT ATGCA^T
HpaI 1469 GTTAAC GTT^AAC
MflI 114 GGATCC [GA]^GATC[CT]
MluNI 3091 TGGCCA TGG^CCA
MscI 3091 TGGCCA TGG^CCA
MvnI 47 CGCG CG^CG
NheI 2642 GCTAGC G^CTAGC
NsiI 711 ATGCAT ATGCA^T
PinAI 1017 ACCGGT A^CCGGT
PmaCI 3087 CACGTG CAC^GTG
PmlI 3087 CACGTG CAC^GTG
PshAI 2238 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1472 AACGTT AA^CGTT
PstI 3080 CTGCAG CTGCA^G
SapI 1455 GCTCTTC GCTCTTC(1/4)
SexAI 2930 ACCTGGT A^CC[AT]GGT
SpeI 1854 ACTAGT A^CTAGT
XhoII 114 GGATCC [GA]^GATC[CT]
XmnI 2869 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 115 GATCC GGATC(4/5)
114 GGATC
AocI 618 CCTCAGG CC^T[ACGT]AGG
1224 CCTCAGG
BsmI 1987 GAATGC GAATGC(1/-1)
2808 GAATGC
BspMI 1809 GCAGGT ACCTGC(4/8)
3039 GCAGGT
BstBI 264 TTCGAA TT^CGAA
744 TTCGAA
BstXI 637 CCACATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1356 CCATCGGACTGG
Bsu36I 618 CCTCAGG CC^T[ACGT]AGG
1224 CCTCAGG
CfoI 46 GCGC GCG^C
2419 GCGC
DrdI 760 GACCAACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2754 GACGGGAAAGTC
Eco81I 618 CCTCAGG CC^T[ACGT]AGG
1224 CCTCAGG
EcoRI 1256 GAATTC G^AATTC
1587 GAATTC
HhaI 46 GCGC GCG^C
2419 GCGC
HinP1I 46 GCGC G^CGC
2419 GCGC
HincII 139 GTCAAC GT[CT]^[GA]AC
1469 GTTAAC
HindII 139 GTCAAC GT[CT]^[GA]AC
1469 GTTAAC
NcoI 477 CCATGG C^CATGG
1615 CCATGG
NspI 765 ACATGT [GA]CATG^[CT]
2601 ACATGC
NspV 264 TTCGAA TT^CGAA
744 TTCGAA
PaeR7I 105 CTCGAG C^TCGAG
876 CTCGAG
SfuI 264 TTCGAA TT^CGAA
744 TTCGAA
SmaI 231 CCCGGG CCC^GGG
360 CCCGGG
XcmI 634 CCACCACATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2108 CCAGCTCTCTTCTGG
XhoI 105 CTCGAG C^TCGAG
876 CTCGAG
XmaCI 231 CCCGGG C^CCGGG
360 CCCGGG
XmaI 231 CCCGGG C^CCGGG
360 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CelII
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I