##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccI AclI AgeI AscI AseI
AsnI AvrII BbeI BcgI BclI
BglII BlnI BsmI BspDI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco52I EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KasI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SexAI SfoI SfuI
SgrAI SnaBI SphI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 5086 GACGTC GACGT^C
Acc65I 1463 GGTACC G^GTACC
AccIII 4679 TCCGGA T^CCGGA
AcsI 798 GAATTC [GA]^AATT[CT]
AflII 220 CTTAAG C^TTAAG
AhdI 3771 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 798 GAATTC [GA]^AATT[CT]
Asp700I 878 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1463 GGTACC G^GTACC
AspEI 3771 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 5115 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5195 TGCGCA TGC^GCA
BamHI 1686 GGATCC G^GATCC
BbsI 2150 GAAGAC GAAGAC(2/6)
BciVI 5415 GGATAC GTATCC(6/5)
BfrI 220 CTTAAG C^TTAAG
BpuAI 2150 GAAGAC GAAGAC(2/6)
BsaBI 3471 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4679 TCCGGA [AT]^CCGG[AT]
BseAI 4679 TCCGGA T^CCGGA
BsiEI 3140 CGACCG CG[GA][CT]^CG
BsiWI 3774 CGTACG C^GTACG
BspEI 4679 TCCGGA T^CCGGA
BspHI 2616 TCATGA T^CATGA
BspLU11I 5403 ACATGT A^CATGT
BssHII 5456 GCGCGC G^CGCGC
DraI 109 TTTAAA TTT^AAA
Eam1105I 3771 GACCGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 798 GAATTC G^AATTC
FspI 5195 TGCGCA TGC^GCA
HindIII 5295 AAGCTT A^AGCTT
KpnI 1463 GGTACC GGTAC^C
KspI 4278 CCGCGG CCGC^GG
MamI 3471 GATGTCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4679 TCCGGA T^CCGGA
PaeR7I 1190 CTCGAG C^TCGAG
PflFI 5115 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2616 TCATGA T^CATGA
SacII 4278 CCGCGG CCGC^GG
SbfI 2883 CCTGCAGG CCTGCA^GG
ScaI 4742 AGTACT AGT^ACT
SpeI 1699 ACTAGT A^CTAGT
Sse8387I 2883 CCTGCAGG CCTGCA^GG
Tth111I 5115 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1190 CTCGAG C^TCGAG
XmnI 878 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Aor51HI 4061 AGCGCT AGC^GCT
5060 AGCGCT
BbrPI 5323 CACGTG CAC^GTG
5394 CACGTG
BsmBI 4366 CGTCTC CGTCTC(1/5)
4570 GAGACG
BsrDI 4902 CATTGC GCAATG(2/0)
4922 GCAATG
BsrFI 3383 ACCGGC [GA]^CCGG[CT]
4178 ACCGGC
BstEII 2181 GGTGACC G^GT[ACGT]ACC
3357 GGTGACC
BstPI 2181 GGTGACC G^GT[ACGT]ACC
3357 GGTGACC
Cfr10I 3383 ACCGGC [GA]^CCGG[CT]
4178 ACCGGC
Eco47III 4061 AGCGCT AGC^GCT
5060 AGCGCT
EcoO65I 2181 GGTGACC G^GT[ACGT]ACC
3357 GGTGACC
NcoI 1568 CCATGG C^CATGG
5165 CCATGG
NspI 4447 GCATGT [GA]CATG^[CT]
5403 ACATGT
PmaCI 5323 CACGTG CAC^GTG
5394 CACGTG
PmlI 5323 CACGTG CAC^GTG
5394 CACGTG
SapI 4621 GAAGAGC GCTCTTC(1/4)
4339 GCTCTTC
SspI 236 AATATT AAT^ATT
3578 AATATT
TfiI 747 GAATC G^A[AT]TC
3565 GATTC
Tsp509I 799 AATT ^AATT
1361 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI Alw44I AlwI AlwNI AocI
ApaI ApaLI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrGI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SmaI SmlI
SspBI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI