##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII BamHI BcgI
BfrI BglII BsaBI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I ClaI CpoI DraIII
Eam1105I Eco47III EcoO65I EcoRI EcoRV
FseI FspI HincII HindII HindIII
HpaI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
ScaI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 1019 AAATTT [GA]^AATT[CT]
AocI 725 CCTGAGG CC^T[ACGT]AGG
ApoI 1019 AAATTT [GA]^AATT[CT]
BbeI 531 GGCGCC GGCGC^C
BbrPI 429 CACGTG CAC^GTG
BbsI 1411 GAAGAC GAAGAC(2/6)
BciVI 1459 GTATCC GTATCC(6/5)
BclI 4249 TGATCA T^GATCA
BpuAI 1411 GAAGAC GAAGAC(2/6)
BsaAI 429 CACGTG [CT]AC^GT[GA]
BsgI 3681 CTGCAC GTGCAG(16/14)
BsmI 1177 GCATTC GAATGC(1/-1)
BspHI 4121 TCATGA T^CATGA
BsrDI 3276 GCAATG GCAATG(2/0)
Bsu36I 725 CCTGAGG CC^T[ACGT]AGG
DrdI 2797 GACTTTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 725 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1175 ATGCAT ATGCA^T
FbaI 4249 TGATCA T^GATCA
KasI 531 GGCGCC G^GCGCC
NaeI 75 GCCGGC GCC^GGC
NarI 531 GGCGCC GG^CGCC
NgoMIV 75 GCCGGC G^CCGGC
NsiI 1175 ATGCAT ATGCA^T
PmaCI 429 CACGTG CAC^GTG
PmlI 429 CACGTG CAC^GTG
RcaI 4121 TCATGA T^CATGA
SfiI 1198 GGCCAACCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 531 GGCGCC GGC^GCC
SspI 4186 AATATT AAT^ATT
TaqI 1600 TCGA T^CGA
TthHB8I 1600 TCGA T^CGA
XbaI 1620 TCTAGA T^CTAGA
XcmI 1639 CCAGGCACGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 597 GGTACC G^GTACC
2864 GGTACC
Asp718I 597 GGTACC G^GTACC
2864 GGTACC
AvrII 453 CCTAGG C^CTAGG
4064 CCTAGG
BlnI 453 CCTAGG C^CTAGG
4064 CCTAGG
BlpI 977 GCTCAGC GC^T[ACGT]AGC
2501 GCTTAGC
Bpu1102I 977 GCTCAGC GC^T[ACGT]AGC
2501 GCTTAGC
BsiEI 369 CGGCCG CG[GA][CT]^CG
1287 CGGCCG
BspLU11I 1770 ACATGT A^CATGT
3504 ACATGT
BspMI 1343 ACCTGC ACCTGC(4/8)
1508 ACCTGC
BstXI 1372 CCAGGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2227 CCATCATCATGG
BstYI 1630 AGATCC [GA]^GATC[CT]
2564 GGATCT
CelII 977 GCTCAGC GC^T[ACGT]AGC
2501 GCTTAGC
EagI 369 CGGCCG C^GGCCG
1287 CGGCCG
EarI 1274 CTCTTC CTCTTC(1/4)
1400 CTCTTC
EclXI 369 CGGCCG C^GGCCG
1287 CGGCCG
Eco52I 369 CGGCCG C^GGCCG
1287 CGGCCG
HaeII 379 AGCGCC [GA]GCGC^[CT]
531 GGCGCC
KpnI 597 GGTACC GGTAC^C
2864 GGTACC
Ksp632I 1274 CTCTTC CTCTTC(1/4)
1400 CTCTTC
KspI 366 CCGCGG CCGC^GG
391 CCGCGG
MaeII 430 ACGT A^CGT
3809 ACGT
MflI 1630 AGATCC [GA]^GATC[CT]
2564 GGATCT
NspI 1770 ACATGT [GA]CATG^[CT]
3504 ACATGT
PflMI 932 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1477 CCAGCCCGTGG
PpuMI 3114 GGGTCCC [GA]G^G[AT]CC[CT]
3174 GGGACCC
PshAI 276 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2053 GACCCCAGTC
PvuII 1914 CAGCTG CAG^CTG
1920 CAGCTG
SacII 366 CCGCGG CCGC^GG
391 CCGCGG
SexAI 3484 ACCAGGT A^CC[AT]GGT
3693 ACCTGGT
SmaI 302 CCCGGG CCC^GGG
4107 CCCGGG
StuI 1429 AGGCCT AGG^CCT
2019 AGGCCT
Van91I 932 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1477 CCAGCCCGTGG
XhoII 1630 AGATCC [GA]^GATC[CT]
2564 GGATCT
XmaCI 302 CCCGGG C^CCGGG
4107 CCCGGG
XmaI 302 CCCGGG C^CCGGG
4107 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrBI BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI