##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AhdI AocI AscI Asp700I Asp718I
AspEI BcgI BciVI BfrI BglII
BseAI BsiWI BspDI BspEI BssHII
Bst1107I BstBI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I Eco81I
EcoRV HgaI HincII HindII HindIII
HpaI KpnI MfeI MluI MroI
MunI NheI NruI NspV PacI
PmeI Psp1406I PvuI RsrII SalI
SexAI SfiI SfuI SnaBI SpeI
SphI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AatII 270 GACGTC GACGT^C
AgeI 2144 ACCGGT A^CCGGT
AseI 2816 ATTAAT AT^TAAT
AsnI 2816 ATTAAT AT^TAAT
BlpI 1475 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1475 GCTCAGC GC^T[ACGT]AGC
BsaBI 1950 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 265 GAGACG CGTCTC(1/5)
BsmI 1547 GCATTC GAATGC(1/-1)
Bsp1407I 2696 TGTACA T^GTACA
BspHI 1589 TCATGA T^CATGA
BspLU11I 2402 ACATGT A^CATGT
BsrGI 2696 TGTACA T^GTACA
CelII 1475 GCTCAGC GC^T[ACGT]AGC
EcoNI 1938 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2017 GAATTC G^AATTC
EcoT22I 2290 ATGCAT ATGCA^T
KspI 63 CCGCGG CCGC^GG
MamI 1950 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2740 CATATG CA^TATG
NotI 53 GCGGCCGC GC^GGCCGC
NsiI 2290 ATGCAT ATGCA^T
NspI 2402 ACATGT [GA]CATG^[CT]
PaeR7I 1249 CTCGAG C^TCGAG
PinAI 2144 ACCGGT A^CCGGT
PpuMI 1679 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 2816 ATTAAT AT^TAAT
RcaI 1589 TCATGA T^CATGA
SacII 63 CCGCGG CCGC^GG
SapI 2052 GAAGAGC GCTCTTC(1/4)
SgrAI 2569 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 2696 TGTACA T^GTACA
SspI 3180 AATATT AAT^ATT
StuI 2582 AGGCCT AGG^CCT
XhoI 1249 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Aor51HI 130 AGCGCT AGC^GCT
1004 AGCGCT
ApaI 361 GGGCCC GGGCC^C
3007 GGGCCC
AspI 267 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2346 GACCCTGTC
AviII 1905 TGCGCA TGC^GCA
3070 TGCGCA
BalI 824 TGGCCA TGG^CCA
3936 TGGCCA
BamHI 1622 GGATCC G^GATCC
2667 GGATCC
BbrPI 678 CACGTG CAC^GTG
963 CACGTG
BbvCI 957 CCTCAGC CCTCAGC(-5/-2)
1898 CCTCAGC
BclI 1112 TGATCA T^GATCA
1406 TGATCA
BsrDI 632 GCAATG GCAATG(2/0)
968 GCAATG
BstXI 1724 CCAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2022 CCAGGCCAGTGG
DraIII 1909 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
2038 CACTACGTG
Eco47III 130 AGCGCT AGC^GCT
1004 AGCGCT
FbaI 1112 TGATCA T^GATCA
1406 TGATCA
FseI 1276 GGCCGGCC GGCCGG^CC
1280 GGCCGGCC
FspI 1905 TGCGCA TGC^GCA
3070 TGCGCA
MluNI 824 TGGCCA TGG^CCA
3936 TGGCCA
MscI 824 TGGCCA TGG^CCA
3936 TGGCCA
PflFI 267 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2346 GACCCTGTC
PflMI 286 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1695 CCACCTGCTGG
PmaCI 678 CACGTG CAC^GTG
963 CACGTG
PmlI 678 CACGTG CAC^GTG
963 CACGTG
PshAI 200 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
425 GACCCGAGTC
SbfI 1121 CCTGCAGG CCTGCA^GG
3567 CCTGCAGG
ScaI 1364 AGTACT AGT^ACT
2833 AGTACT
SmaI 395 CCCGGG CCC^GGG
3056 CCCGGG
Sse8387I 1121 CCTGCAGG CCTGCA^GG
3567 CCTGCAGG
Tth111I 267 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2346 GACCCTGTC
Van91I 286 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1695 CCACCTGCTGG
XmaCI 395 CCCGGG C^CCGGG
3056 CCCGGG
XmaI 395 CCCGGG C^CCGGG
3056 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI AspHI AvaI AvaII
AvrII BanI BanII BbeI BbsI
BbvI BcnI BfaI BglI BlnI
BmyI BpmI BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PleI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII