##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII Acc65I Alw44I Aor51HI ApaLI
AseI AsnI Asp700I Asp718I AviII
BamHI BlpI Bpu1102I BsiWI Bsp1407I
BspDI BsrGI BstBI CelII ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoRV FseI FspI
HindIII HpaI KpnI MfeI MluI
MunI NdeI NheI NotI NruI
NspV PacI PmeI PshBI RsrII
SacI SalI ScaI SexAI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccIII 2548 TCCGGA T^CCGGA
AclI 3986 AACGTT AA^CGTT
AcsI 3302 GAATTC [GA]^AATT[CT]
AgeI 3227 ACCGGT A^CCGGT
AocI 2414 CCTGAGG CC^T[ACGT]AGG
ApoI 3302 GAATTC [GA]^AATT[CT]
AscI 1016 GGCGCGCC GG^CGCGCC
AspI 4658 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4936 CCTAGG C^CTAGG
BbrPI 3328 CACGTG CAC^GTG
BcgI 3274 CGATGCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2361 AGATCT A^GATCT
BlnI 4936 CCTAGG C^CTAGG
BsaAI 3328 CACGTG [CT]AC^GT[GA]
BsaBI 5337 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2548 TCCGGA T^CCGGA
BsiEI 5359 CGATCG CG[GA][CT]^CG
BsmBI 4656 GAGACG CGTCTC(1/5)
BspEI 2548 TCCGGA T^CCGGA
BspHI 4575 TCATGA T^CATGA
BsrDI 5117 CATTGC GCAATG(2/0)
BssSI 966 CACGAG CACGAG(-5/-1)
Bst1107I 5050 GTATAC GTA^TAC
BstZ17I 5050 GTATAC GTA^TAC
Bsu36I 2414 CCTGAGG CC^T[ACGT]AGG
Eco81I 2414 CCTGAGG CC^T[ACGT]AGG
EcoRI 3302 GAATTC G^AATTC
EcoT22I 3969 ATGCAT ATGCA^T
HincII 2604 GTCAAC GT[CT]^[GA]AC
HindII 2604 GTCAAC GT[CT]^[GA]AC
MamI 5337 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2548 TCCGGA T^CCGGA
NsiI 3969 ATGCAT ATGCA^T
PflFI 4658 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3227 ACCGGT A^CCGGT
PmaCI 3328 CACGTG CAC^GTG
PmlI 3328 CACGTG CAC^GTG
PshAI 5213 GACATGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3986 AACGTT AA^CGTT
PvuI 5359 CGATCG CGAT^CG
RcaI 4575 TCATGA T^CATGA
SphI 2947 GCATGC GCATG^C
Tth111I 4658 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 599 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 4755 ACATGT A^C[GA][CT]GT
5214 ACATGT
AhdI 2300 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5357 GACGATCGGTC
AspEI 2300 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5357 GACGATCGGTC
BbeI 1141 GGCGCC GGCGC^C
3953 GGCGCC
BbvCI 4354 CCTCAGC CCTCAGC(-5/-2)
4645 GCTGAGG
BciVI 2740 GGATAC GTATCC(6/5)
5272 GTATCC
BclI 2407 TGATCA T^GATCA
5230 TGATCA
BsmI 521 GCATTC GAATGC(1/-1)
3590 GCATTC
BspLU11I 4755 ACATGT A^CATGT
5214 ACATGT
BstEII 1955 GGTCACC G^GT[ACGT]ACC
3223 GGTCACC
BstPI 1955 GGTCACC G^GT[ACGT]ACC
3223 GGTCACC
Eam1105I 2300 GACAGCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5357 GACGATCGGTC
EcoO65I 1955 GGTCACC G^GT[ACGT]ACC
3223 GGTCACC
FbaI 2407 TGATCA T^GATCA
5230 TGATCA
KasI 1141 GGCGCC G^GCGCC
3953 GGCGCC
NaeI 1221 GCCGGC GCC^GGC
2508 GCCGGC
NarI 1141 GGCGCC GG^CGCC
3953 GGCGCC
NcoI 4680 CCATGG C^CATGG
4765 CCATGG
NgoMIV 1221 GCCGGC G^CCGGC
2508 GCCGGC
PaeR7I 2102 CTCGAG C^TCGAG
4484 CTCGAG
SapI 1314 GAAGAGC GCTCTTC(1/4)
446 GCTCTTC
SbfI 350 CCTGCAGG CCTGCA^GG
1340 CCTGCAGG
SfiI 1776 GGCCACCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4718 GGCCACAGGGGCC
SfoI 1141 GGCGCC GGC^GCC
3953 GGCGCC
Sse8387I 350 CCTGCAGG CCTGCA^GG
1340 CCTGCAGG
Tsp509I 3303 AATT ^AATT
3791 AATT
XhoI 2102 CTCGAG C^TCGAG
4484 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflII AluI AlwI AlwNI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BfrI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI