##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccIII AcsI AgeI ApoI AscI
AvrII BamHI BbeI BclI BglII
BlnI BsaBI BseAI BsiWI BsmI
BspDI BspEI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco52I EcoRI EcoRV EcoT22I
FbaI FseI HpaI KasI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PvuI RsrII SfoI SfuI SpeI
SphI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AclI 330 AACGTT AA^CGTT
AflII 2511 CTTAAG C^TTAAG
AhdI 381 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 1919 ATTAAT AT^TAAT
AsnI 1919 ATTAAT AT^TAAT
Asp700I 435 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 381 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3993 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 3153 GTATCC GTATCC(6/5)
BfrI 2511 CTTAAG C^TTAAG
BsaI 539 GGTCTC GGTCTC(1/5)
BsaWI 3610 TCCGGT [AT]^CCGG[AT]
BsiEI 164 CGACCG CG[GA][CT]^CG
Eam1105I 381 GACGTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HindIII 4765 AAGCTT A^AGCTT
PflFI 3993 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1919 ATTAAT AT^TAAT
Psp1406I 330 AACGTT AA^CGTT
SbfI 2813 CCTGCAGG CCTGCA^GG
SexAI 5215 ACCTGGT A^CC[AT]GGT
SfiI 5232 GGCCAGGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1394 CACCGGCG C[GA]^CCGG[CT]G
SnaBI 1894 TACGTA TAC^GTA
Sse8387I 2813 CCTGCAGG CCTGCA^GG
Tth111I 3993 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 435 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Aor51HI 3818 AGCGCT AGC^GCT
4595 AGCGCT
AviII 5274 TGCGCA TGC^GCA
5527 TGCGCA
BfaI 2520 CTAG C^TAG
4011 CTAG
Bsp1407I 1171 TGTACA T^GTACA
1525 TGTACA
BsrDI 1676 CATTGC GCAATG(2/0)
4467 CATTGC
BsrGI 1171 TGTACA T^GTACA
1525 TGTACA
BstEII 501 GGTGACC G^GT[ACGT]ACC
5474 GGTGACC
BstPI 501 GGTGACC G^GT[ACGT]ACC
5474 GGTGACC
BstXI 1636 CCAAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2677 CCATTAACTTGG
Eco47III 3818 AGCGCT AGC^GCT
4595 AGCGCT
EcoO65I 501 GGTGACC G^GT[ACGT]ACC
5474 GGTGACC
FspI 5274 TGCGCA TGC^GCA
5527 TGCGCA
HincII 2187 GTCGAC GT[CT]^[GA]AC
4651 GTCGAC
HindII 2187 GTCGAC GT[CT]^[GA]AC
4651 GTCGAC
KspI 5388 CCGCGG CCGC^GG
5492 CCGCGG
MaeI 2520 CTAG C^TAG
4011 CTAG
SacII 5388 CCGCGG CCGC^GG
5492 CCGCGG
SalI 2187 GTCGAC G^TCGAC
4651 GTCGAC
SapI 488 GCTCTTC GCTCTTC(1/4)
545 GCTCTTC
ScaI 2766 AGTACT AGT^ACT
5446 AGTACT
SspBI 1171 TGTACA T^GTACA
1525 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AatII Acc65I AccI AccII AciI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI AocI ApaI ApaLI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BcgI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspLU11I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PshAI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI