##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AseI AsnI Asp700I AspEI
BfrI BseAI BsiWI BsmI BspDI
BspEI BspLU11I Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
EcoRV HpaI MluI MroI MseI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SacI SbfI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tru9I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Alw44I 4607 GTGCAC G^TGCAC
Aor51HI 4446 AGCGCT AGC^GCT
ApaLI 4607 GTGCAC G^TGCAC
AscI 291 GGCGCGCC GG^CGCGCC
AspI 2257 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 796 TGCGCA TGC^GCA
AvrII 4201 CCTAGG C^CTAGG
BcgI 1786 GCATTTTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2672 GTATCC GTATCC(6/5)
BclI 3050 TGATCA T^GATCA
BglII 4093 AGATCT A^GATCT
BlnI 4201 CCTAGG C^CTAGG
BsaBI 2337 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 1414 TGTACA T^GTACA
BspHI 4795 TCATGA T^CATGA
BsrGI 1414 TGTACA T^GTACA
BssSI 1724 CACGAG CACGAG(-5/-1)
BstEII 2247 GGTGACC G^GT[ACGT]ACC
BstPI 2247 GGTGACC G^GT[ACGT]ACC
DraIII 4556 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 4446 AGCGCT AGC^GCT
EcoO65I 2247 GGTGACC G^GT[ACGT]ACC
EcoRI 2836 GAATTC G^AATTC
EcoT22I 2629 ATGCAT ATGCA^T
FbaI 3050 TGATCA T^GATCA
FspI 796 TGCGCA TGC^GCA
HincII 2263 GTCGAC GT[CT]^[GA]AC
HindII 2263 GTCGAC GT[CT]^[GA]AC
HindIII 2731 AAGCTT A^AGCTT
MamI 2337 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1002 CAATTG C^AATTG
MunI 1002 CAATTG C^AATTG
NsiI 2629 ATGCAT ATGCA^T
PflFI 2257 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2020 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4795 TCATGA T^CATGA
SalI 2263 GTCGAC G^TCGAC
ScaI 348 AGTACT AGT^ACT
SfiI 528 GGCCGGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3624 GCATGC GCATG^C
SspBI 1414 TGTACA T^GTACA
SspI 2807 AATATT AAT^ATT
TfiI 3478 GAATC G^A[AT]TC
Tth111I 2257 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 1955 GAATTT [GA]^AATT[CT]
2836 GAATTC
AflIII 2295 ACACGT A^C[GA][CT]GT
4875 ACGTGT
ApoI 1955 GAATTT [GA]^AATT[CT]
2836 GAATTC
BamHI 3158 GGATCC G^GATCC
3305 GGATCC
BbeI 366 GGCGCC GGCGC^C
3259 GGCGCC
BbrPI 3208 CACGTG CAC^GTG
4874 CACGTG
BlpI 581 GCTGAGC GC^T[ACGT]AGC
2135 GCTGAGC
Bpu1102I 581 GCTGAGC GC^T[ACGT]AGC
2135 GCTGAGC
BsaAI 3208 CACGTG [CT]AC^GT[GA]
4874 CACGTG
BsaWI 73 ACCGGA [AT]^CCGG[AT]
432 TCCGGT
BsmBI 4250 CGTCTC CGTCTC(1/5)
4433 GAGACG
BsrDI 1268 CATTGC GCAATG(2/0)
4483 CATTGC
BssHII 292 GCGCGC G^CGCGC
586 GCGCGC
CelII 581 GCTGAGC GC^T[ACGT]AGC
2135 GCTGAGC
KasI 366 GGCGCC G^GCGCC
3259 GGCGCC
NarI 366 GGCGCC GG^CGCC
3259 GGCGCC
NspI 3624 GCATGC [GA]CATG^[CT]
4287 GCATGT
PmaCI 3208 CACGTG CAC^GTG
4874 CACGTG
PmlI 3208 CACGTG CAC^GTG
4874 CACGTG
SapI 1543 GAAGAGC GCTCTTC(1/4)
627 GCTCTTC
SexAI 2873 ACCTGGT A^CC[AT]GGT
3809 ACCAGGT
SfoI 366 GGCGCC GGC^GCC
3259 GGCGCC
XcmI 1325 CCACTGGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2384 CCACCAGCTGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
Acc65I AccI AccII AciI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI Asp718I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FseI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KpnI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI