################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI AseI AsnI Asp700I AspEI BfrI BseAI BsiWI BsmI BspDI BspEI BspLU11I Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI Eam1105I EcoRV HpaI MluI MroI MseI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SbfI SfuI SgrAI SnaBI SpeI Sse8387I SwaI Tru9I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Alw44I 4607 GTGCAC G^TGCAC Aor51HI 4446 AGCGCT AGC^GCT ApaLI 4607 GTGCAC G^TGCAC AscI 291 GGCGCGCC GG^CGCGCC AspI 2257 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 796 TGCGCA TGC^GCA AvrII 4201 CCTAGG C^CTAGG BcgI 1786 GCATTTTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2672 GTATCC GTATCC(6/5) BclI 3050 TGATCA T^GATCA BglII 4093 AGATCT A^GATCT BlnI 4201 CCTAGG C^CTAGG BsaBI 2337 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 1414 TGTACA T^GTACA BspHI 4795 TCATGA T^CATGA BsrGI 1414 TGTACA T^GTACA BssSI 1724 CACGAG CACGAG(-5/-1) BstEII 2247 GGTGACC G^GT[ACGT]ACC BstPI 2247 GGTGACC G^GT[ACGT]ACC DraIII 4556 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 4446 AGCGCT AGC^GCT EcoO65I 2247 GGTGACC G^GT[ACGT]ACC EcoRI 2836 GAATTC G^AATTC EcoT22I 2629 ATGCAT ATGCA^T FbaI 3050 TGATCA T^GATCA FspI 796 TGCGCA TGC^GCA HincII 2263 GTCGAC GT[CT]^[GA]AC HindII 2263 GTCGAC GT[CT]^[GA]AC HindIII 2731 AAGCTT A^AGCTT MamI 2337 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1002 CAATTG C^AATTG MunI 1002 CAATTG C^AATTG NsiI 2629 ATGCAT ATGCA^T PflFI 2257 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2020 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 4795 TCATGA T^CATGA SalI 2263 GTCGAC G^TCGAC ScaI 348 AGTACT AGT^ACT SfiI 528 GGCCGGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SphI 3624 GCATGC GCATG^C SspBI 1414 TGTACA T^GTACA SspI 2807 AATATT AAT^ATT TfiI 3478 GAATC G^A[AT]TC Tth111I 2257 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 1955 GAATTT [GA]^AATT[CT] 2836 GAATTC AflIII 2295 ACACGT A^C[GA][CT]GT 4875 ACGTGT ApoI 1955 GAATTT [GA]^AATT[CT] 2836 GAATTC BamHI 3158 GGATCC G^GATCC 3305 GGATCC BbeI 366 GGCGCC GGCGC^C 3259 GGCGCC BbrPI 3208 CACGTG CAC^GTG 4874 CACGTG BlpI 581 GCTGAGC GC^T[ACGT]AGC 2135 GCTGAGC Bpu1102I 581 GCTGAGC GC^T[ACGT]AGC 2135 GCTGAGC BsaAI 3208 CACGTG [CT]AC^GT[GA] 4874 CACGTG BsaWI 73 ACCGGA [AT]^CCGG[AT] 432 TCCGGT BsmBI 4250 CGTCTC CGTCTC(1/5) 4433 GAGACG BsrDI 1268 CATTGC GCAATG(2/0) 4483 CATTGC BssHII 292 GCGCGC G^CGCGC 586 GCGCGC CelII 581 GCTGAGC GC^T[ACGT]AGC 2135 GCTGAGC KasI 366 GGCGCC G^GCGCC 3259 GGCGCC NarI 366 GGCGCC GG^CGCC 3259 GGCGCC NspI 3624 GCATGC [GA]CATG^[CT] 4287 GCATGT PmaCI 3208 CACGTG CAC^GTG 4874 CACGTG PmlI 3208 CACGTG CAC^GTG 4874 CACGTG SapI 1543 GAAGAGC GCTCTTC(1/4) 627 GCTCTTC SexAI 2873 ACCTGGT A^CC[AT]GGT 3809 ACCAGGT SfoI 366 GGCGCC GGC^GCC 3259 GGCGCC XcmI 1325 CCACTGGCTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2384 CCACCAGCTGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 136 enzymes) # ######################################### Acc65I AccI AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FseI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI