##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
AscI AspEI AviII AvrII BbeI
BglI BlnI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BssHII Bst1107I
BstAPI BstBI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI FseI FspI HaeII KasI
KspI MluI MroI NarI NheI
NotI NruI NspV PacI PinAI
PmeI Psp1406I PvuI RsrII SacII
ScaI SfiI SfoI SfuI SgrAI
SphI SwaI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AatII 5959 GACGTC GACGT^C
Acc65I 3151 GGTACC G^GTACC
AflII 2008 CTTAAG C^TTAAG
AflIII 722 ACACGT A^C[GA][CT]GT
Alw44I 265 GTGCAC G^TGCAC
ApaLI 265 GTGCAC G^TGCAC
AseI 3342 ATTAAT AT^TAAT
AsnI 3342 ATTAAT AT^TAAT
Asp700I 3407 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3151 GGTACC G^GTACC
BcgI 588 CGACTCAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2008 CTTAAG C^TTAAG
BspHI 4602 TCATGA T^CATGA
BsrDI 3538 GCAATG GCAATG(2/0)
BstEII 1988 GGTGACC G^GT[ACGT]ACC
BstPI 1988 GGTGACC G^GT[ACGT]ACC
DraIII 2468 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1988 GGTGACC G^GT[ACGT]ACC
EcoRV 3249 GATATC GAT^ATC
HpaI 4932 GTTAAC GTT^AAC
KpnI 3151 GGTACC GGTAC^C
NdeI 6003 CATATG CA^TATG
PaeR7I 5738 CTCGAG C^TCGAG
PshBI 3342 ATTAAT AT^TAAT
RcaI 4602 TCATGA T^CATGA
SalI 6033 GTCGAC G^TCGAC
SbfI 4866 CCTGCAGG CCTGCA^GG
SexAI 4381 ACCTGGT A^CC[AT]GGT
SnaBI 1120 TACGTA TAC^GTA
SpeI 4456 ACTAGT A^CTAGT
Sse8387I 4866 CCTGCAGG CCTGCA^GG
SspI 2941 AATATT AAT^ATT
XhoI 5738 CTCGAG C^TCGAG
XmnI 3407 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 5962 GTCTAC GT^[AC][GT]AC
6033 GTCGAC
AccII 982 CGCG CG^CG
4188 CGCG
BbrPI 723 CACGTG CAC^GTG
3494 CACGTG
BciVI 1875 GTATCC GTATCC(6/5)
3935 GTATCC
BclI 570 TGATCA T^GATCA
3530 TGATCA
BglII 3409 AGATCT A^GATCT
5710 AGATCT
BsaBI 2361 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5577 GATCGTGATC
BsaWI 525 ACCGGA [AT]^CCGG[AT]
4062 ACCGGA
BsmBI 4811 CGTCTC CGTCTC(1/5)
787 GAGACG
BsmI 5169 GAATGC GAATGC(1/-1)
6079 GCATTC
Bsp1407I 3842 TGTACA T^GTACA
6065 TGTACA
BsrBI 89 CCGCTC CCGCTC(-3/-3)
3760 CCGCTC
BsrFI 26 GCCGGC [GA]^CCGG[CT]
274 GCCGGC
BsrGI 3842 TGTACA T^GTACA
6065 TGTACA
BssSI 262 CTCGTG CACGAG(-5/-1)
4090 CTCGTG
BstUI 982 CGCG CG^CG
4188 CGCG
Cfr10I 26 GCCGGC [GA]^CCGG[CT]
274 GCCGGC
DrdI 2 GACTGGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5882 GACTGAGAAGTC
EcoT22I 2158 ATGCAT ATGCA^T
2557 ATGCAT
FbaI 570 TGATCA T^GATCA
3530 TGATCA
HincII 4932 GTTAAC GT[CT]^[GA]AC
6033 GTCGAC
HindII 4932 GTTAAC GT[CT]^[GA]AC
6033 GTCGAC
HindIII 5763 AAGCTT A^AGCTT
6106 AAGCTT
MamI 2361 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5577 GATCGTGATC
MvnI 982 CGCG CG^CG
4188 CGCG
NaeI 26 GCCGGC GCC^GGC
274 GCCGGC
NcoI 1951 CCATGG C^CATGG
4594 CCATGG
NgoMIV 26 GCCGGC G^CCGGC
274 GCCGGC
NsiI 2158 ATGCAT ATGCA^T
2557 ATGCAT
NspI 2247 ACATGC [GA]CATG^[CT]
6062 GCATGT
PmaCI 723 CACGTG CAC^GTG
3494 CACGTG
PmlI 723 CACGTG CAC^GTG
3494 CACGTG
PshAI 810 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2955 GACAAGGGTC
SapI 83 GAAGAGC GCTCTTC(1/4)
6013 GAAGAGC
SmaI 608 CCCGGG CCC^GGG
1511 CCCGGG
SspBI 3842 TGTACA T^GTACA
6065 TGTACA
XbaI 3670 TCTAGA T^CTAGA
5895 TCTAGA
XmaCI 608 CCCGGG C^CCGGG
1511 CCCGGG
XmaI 608 CCCGGG C^CCGGG
1511 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AspI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII