##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AccIII AclI AscI AseI AsnI
AviII AvrII BbeI BcgI BciVI
BglI BlnI BseAI BspDI BspEI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DrdI EcoRV FseI FspI
HpaI KasI MroI NarI NheI
NotI NruI NspV PacI PaeR7I
PmeI PshAI PshBI Psp1406I PvuI
PvuII RsrII SfiI SfoI SfuI
SgrAI SpeI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 1722 GACGTC GACGT^C
Acc65I 4012 GGTACC G^GTACC
AflII 3289 CTTAAG C^TTAAG
AgeI 666 ACCGGT A^CCGGT
Asp718I 4012 GGTACC G^GTACC
BalI 1470 TGGCCA TGG^CCA
BamHI 2600 GGATCC G^GATCC
BfrI 3289 CTTAAG C^TTAAG
BglII 1302 AGATCT A^GATCT
BsiEI 5262 CGGCCG CG[GA][CT]^CG
Bsp1407I 5061 TGTACA T^GTACA
BspHI 3344 TCATGA T^CATGA
BsrGI 5061 TGTACA T^GTACA
EagI 5262 CGGCCG C^GGCCG
EclXI 5262 CGGCCG C^GGCCG
Eco52I 5262 CGGCCG C^GGCCG
HindIII 2854 AAGCTT A^AGCTT
KpnI 4012 GGTACC GGTAC^C
MfeI 316 CAATTG C^AATTG
MluI 728 ACGCGT A^CGCGT
MluNI 1470 TGGCCA TGG^CCA
MscI 1470 TGGCCA TGG^CCA
MunI 316 CAATTG C^AATTG
NcoI 1467 CCATGG C^CATGG
NdeI 4992 CATATG CA^TATG
PinAI 666 ACCGGT A^CCGGT
RcaI 3344 TCATGA T^CATGA
SalI 1583 GTCGAC G^TCGAC
ScaI 2023 AGTACT AGT^ACT
SmaI 1756 CCCGGG CCC^GGG
SnaBI 4782 TACGTA TAC^GTA
SphI 5986 GCATGC GCATG^C
SspBI 5061 TGTACA T^GTACA
SspI 3840 AATATT AAT^ATT
StuI 3318 AGGCCT AGG^CCT
XcmI 28 CCAGCGCTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1756 CCCGGG C^CCGGG
XmaI 1756 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 1722 GACGTC G[GA]^CG[CT]C
1760 GGCGTC
AhdI 724 GACCACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1066 GACCTCTTGTC
Alw44I 3181 GTGCAC G^TGCAC
4929 GTGCAC
ApaI 2211 GGGCCC GGGCC^C
2246 GGGCCC
ApaLI 3181 GTGCAC G^TGCAC
4929 GTGCAC
Asp700I 1604 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3471 GAACTATTTC
AspEI 724 GACCACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1066 GACCTCTTGTC
BbrPI 919 CACGTG CAC^GTG
1915 CACGTG
BsaBI 3526 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3782 GATAGTGATC
BsaHI 1722 GACGTC G[GA]^CG[CT]C
1760 GGCGTC
BsiWI 3718 CGTACG C^GTACG
4954 CGTACG
BsmI 333 GAATGC GAATGC(1/-1)
4945 GCATTC
BspLU11I 3350 ACATGT A^CATGT
4933 ACATGT
BsrBI 1740 CCGCTC CCGCTC(-3/-3)
1121 GAGCGG
BsrDI 5213 CATTGC GCAATG(2/0)
2557 GCAATG
BssSI 4417 CACGAG CACGAG(-5/-1)
5981 CTCGTG
BstAPI 2326 GCAGGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4747 GCAGTCTGTGC
BstXI 1701 CCAGGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4386 CCAGCACTTTGG
DraIII 1699 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2297 CACTCAGTG
Eam1105I 724 GACCACGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1066 GACCTCTTGTC
Hin1I 1722 GACGTC G[GA]^CG[CT]C
1760 GGCGTC
HincII 1583 GTCGAC GT[CT]^[GA]AC
6242 GTTGAC
HindII 1583 GTCGAC GT[CT]^[GA]AC
6242 GTTGAC
KspI 1261 CCGCGG CCGC^GG
3141 CCGCGG
MamI 3526 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3782 GATAGTGATC
NaeI 1623 GCCGGC GCC^GGC
2968 GCCGGC
NgoMIV 1623 GCCGGC G^CCGGC
2968 GCCGGC
PmaCI 919 CACGTG CAC^GTG
1915 CACGTG
PmlI 919 CACGTG CAC^GTG
1915 CACGTG
PpuMI 758 AGGACCC [GA]G^G[AT]CC[CT]
3928 AGGTCCC
SacII 1261 CCGCGG CCGC^GG
3141 CCGCGG
SbfI 1398 CCTGCAGG CCTGCA^GG
1939 CCTGCAGG
Sse8387I 1398 CCTGCAGG CCTGCA^GG
1939 CCTGCAGG
XmnI 1604 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3471 GAACTATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI ApoI AspHI AspI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BclI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I EcoT22I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflFI PflMI PleI PstI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII