##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AcyI AflII AocI
Aor51HI AscI Asp700I Asp718I AspI
BanI BbeI BbrPI BbvCI BfrI
BglI BlpI Bpu1102I BsaHI BsaI
BsiEI BsiWI BsmBI BspDI BsrBI
BssHII BssSI Bsu36I CelII ClaI
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FseI HaeII Hin1I KasI
KpnI KspI MfeI MluI MspA1I
MunI NaeI NarI NgoMIV NotI
NruI NsiI PflFI PmaCI PmeI
PmlI PpuMI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SmaI Sse8387I SwaI
Tth111I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 1293 GTATAC GT^[AC][GT]AC
AccII 1181 CGCG CG^CG
AgeI 1044 ACCGGT A^CCGGT
AhdI 261 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2225 GTGCAC G^TGCAC
ApaI 764 GGGCCC GGGCC^C
ApaLI 2225 GTGCAC G^TGCAC
AspEI 261 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3035 TGCGCA TGC^GCA
AvrII 5558 CCTAGG C^CTAGG
BalI 1352 TGGCCA TGG^CCA
BamHI 980 GGATCC G^GATCC
BcgI 705 CGACCTGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3724 GTATCC GTATCC(6/5)
BcnI 767 CCCGG CC^[GC]GG
BlnI 5558 CCTAGG C^CTAGG
BsaAI 3925 TACGTA [CT]AC^GT[GA]
Bsp1407I 2787 TGTACA T^GTACA
BsrFI 1044 ACCGGT [GA]^CCGG[CT]
BsrGI 2787 TGTACA T^GTACA
Bst1107I 1293 GTATAC GTA^TAC
BstAPI 3079 GCAAAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1263 TTCGAA TT^CGAA
BstEII 1142 GGTTACC G^GT[ACGT]ACC
BstPI 1142 GGTTACC G^GT[ACGT]ACC
BstUI 1181 CGCG CG^CG
BstZ17I 1293 GTATAC GTA^TAC
Cfr10I 1044 ACCGGT [GA]^CCGG[CT]
EaeI 1352 TGGCCA [CT]^GGCC[GA]
Eam1105I 261 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1142 GGTTACC G^GT[ACGT]ACC
FauI 751 GCGGG CCCGC(4/6)
FspI 3035 TGCGCA TGC^GCA
HgaI 382 GACGC GACGC(5/10)
MluNI 1352 TGGCCA TGG^CCA
MscI 1352 TGGCCA TGG^CCA
MvnI 1181 CGCG CG^CG
NciI 767 CCCGG CC^[GC]GG
NdeI 5090 CATATG CA^TATG
NspV 1263 TTCGAA TT^CGAA
PacI 1625 TTAATTAA TTAAT^TAA
PaeR7I 4666 CTCGAG C^TCGAG
PinAI 1044 ACCGGT A^CCGGT
PshAI 3789 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 756 GAAGAGC GCTCTTC(1/4)
SexAI 3361 ACCAGGT A^CC[AT]GGT
SfuI 1263 TTCGAA TT^CGAA
SgrAI 1043 CACCGGTG C[GA]^CCGG[CT]G
SnaBI 3925 TACGTA TAC^GTA
SpeI 3456 ACTAGT A^CTAGT
SphI 2572 GCATGC GCATG^C
SspBI 2787 TGTACA T^GTACA
XcmI 663 CCAAGAGTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4666 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 1445 TCCGGA T^CCGGA
1529 TCCGGA
AclI 2514 AACGTT AA^CGTT
4800 AACGTT
AlwNI 1303 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
1922 CAGTCTCTG
AseI 1624 ATTAAT AT^TAAT
4342 ATTAAT
AsnI 1624 ATTAAT AT^TAAT
4342 ATTAAT
AspHI 1020 GTGCTC G[AT]GC[AT]^C
2225 GTGCAC
AvaI 812 CCCGAG C^[CT]CG[GA]G
4666 CTCGAG
BanII 764 GGGCCC G[GA]GC[CT]^C
4606 GAGCCC
BclI 311 TGATCA T^GATCA
1310 TGATCA
BseAI 1445 TCCGGA T^CCGGA
1529 TCCGGA
BsiHKAI 1020 GTGCTC G[AT]GC[AT]^C
2225 GTGCAC
BsmI 1805 GAATGC GAATGC(1/-1)
5303 GAATGC
BsoBI 812 CCCGAG C^[CT]CG[GA]G
4666 CTCGAG
BspEI 1445 TCCGGA T^CCGGA
1529 TCCGGA
BspHI 56 TCATGA T^CATGA
3785 TCATGA
BspMI 1519 ACCTGC ACCTGC(4/8)
322 GCAGGT
BsrDI 4206 CATTGC GCAATG(2/0)
3117 GCAATG
BstXI 663 CCAAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1812 CCACTTATCTGG
CfoI 3036 GCGC GCG^C
4210 GCGC
DraII 1254 AGGGCCT [GA]G^G[ACGT]CC[CT]
2623 AGGCCCC
EarI 4373 CTCTTC CTCTTC(1/4)
756 GAAGAG
EcoO109I 1254 AGGGCCT [GA]G^G[ACGT]CC[CT]
2623 AGGCCCC
EcoRI 777 GAATTC G^AATTC
4922 GAATTC
FbaI 311 TGATCA T^GATCA
1310 TGATCA
HhaI 3036 GCGC GCG^C
4210 GCGC
HinP1I 3036 GCGC G^CGC
4210 GCGC
HpaI 633 GTTAAC GTT^AAC
993 GTTAAC
Ksp632I 4373 CTCTTC CTCTTC(1/4)
756 GAAGAG
MroI 1445 TCCGGA T^CCGGA
1529 TCCGGA
NcoI 1355 CCATGG C^CATGG
3511 CCATGG
NheI 1708 GCTAGC G^CTAGC
1768 GCTAGC
PflMI 1147 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5649 CCAAGCCGTGG
PshBI 1624 ATTAAT AT^TAAT
4342 ATTAAT
Psp1406I 2514 AACGTT AA^CGTT
4800 AACGTT
PstI 831 CTGCAG CTGCA^G
2567 CTGCAG
RcaI 56 TCATGA T^CATGA
3785 TCATGA
StuI 255 AGGCCT AGG^CCT
1397 AGGCCT
Van91I 1147 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5649 CCAAGCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaII BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaBI BsaJI BsaWI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspLU11I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DsaI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HindIII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII