##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AclI Alw44I
Aor51HI ApaLI AscI Asp718I AviII
AvrII BbeI BciVI BglII BlnI
BsaBI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BsrBI BssHII BssSI
Bst1107I BstEII BstPI BstZ17I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PmeI PshAI Psp1406I PvuI RsrII
SacI SalI SapI ScaI SfiI
SfoI SgrAI SnaBI SpeI SwaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 4633 GTAGAC GT^[AC][GT]AC
AcyI 3401 GGCGTC G[GA]^CG[CT]C
AflII 512 CTTAAG C^TTAAG
AgeI 2310 ACCGGT A^CCGGT
ApaI 1795 GGGCCC GGGCC^C
AseI 560 ATTAAT AT^TAAT
AsnI 560 ATTAAT AT^TAAT
Asp700I 3752 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 3580 TGGCCA TGG^CCA
BbvCI 3016 GCTGAGG CCTCAGC(-5/-2)
BclI 1447 TGATCA T^GATCA
BfrI 512 CTTAAG C^TTAAG
BglI 1354 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 3401 GGCGTC G[GA]^CG[CT]C
BsrFI 2310 ACCGGT [GA]^CCGG[CT]
BstBI 652 TTCGAA TT^CGAA
Cfr10I 2310 ACCGGT [GA]^CCGG[CT]
EcoT22I 2293 ATGCAT ATGCA^T
FbaI 1447 TGATCA T^GATCA
HgaI 3402 GCGTC GACGC(5/10)
Hin1I 3401 GGCGTC G[GA]^CG[CT]C
HindIII 3961 AAGCTT A^AGCTT
KspI 1504 CCGCGG CCGC^GG
MluNI 3580 TGGCCA TGG^CCA
MscI 3580 TGGCCA TGG^CCA
NsiI 2293 ATGCAT ATGCA^T
NspV 652 TTCGAA TT^CGAA
PaeR7I 1034 CTCGAG C^TCGAG
PinAI 2310 ACCGGT A^CCGGT
PshBI 560 ATTAAT AT^TAAT
SacII 1504 CCGCGG CCGC^GG
SbfI 1355 CCTGCAGG CCTGCA^GG
SexAI 1664 ACCAGGT A^CC[AT]GGT
SfuI 652 TTCGAA TT^CGAA
SphI 2352 GCATGC GCATG^C
Sse8387I 1355 CCTGCAGG CCTGCA^GG
XhoI 1034 CTCGAG C^TCGAG
XmnI 3752 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AhdI 2306 GACAACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4766 GACATGGAGTC
AocI 1295 CCTCAGG CC^T[ACGT]AGG
4178 CCTCAGG
AspEI 2306 GACAACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4766 GACATGGAGTC
AspHI 3361 GAGCAC G[AT]GC[AT]^C
3928 GAGCAC
BbrPI 1861 CACGTG CAC^GTG
1910 CACGTG
BpmI 817 CTGGAG CTGGAG(16/14)
991 CTGGAG
BsaAI 1861 CACGTG [CT]AC^GT[GA]
1910 CACGTG
BsaI 5614 GAGACC GGTCTC(1/5)
4419 GGTCTC
BsaWI 204 ACCGGA [AT]^CCGG[AT]
2310 ACCGGT
BsiHKAI 3361 GAGCAC G[AT]GC[AT]^C
3928 GAGCAC
BspLU11I 3932 ACATGT A^CATGT
4605 ACATGT
BstXI 2189 CCACAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2569 CCAACCTTATGG
Bsu36I 1295 CCTCAGG CC^T[ACGT]AGG
4178 CCTCAGG
Eam1105I 2306 GACAACCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4766 GACATGGAGTC
Eco81I 1295 CCTCAGG CC^T[ACGT]AGG
4178 CCTCAGG
HincII 2717 GTTGAC GT[CT]^[GA]AC
3527 GTCAAC
HindII 2717 GTTGAC GT[CT]^[GA]AC
3527 GTCAAC
PflMI 1839 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5671 CCATCTTGTGG
PmaCI 1861 CACGTG CAC^GTG
1910 CACGTG
PmlI 1861 CACGTG CAC^GTG
1910 CACGTG
SmaI 315 CCCGGG CCC^GGG
1095 CCCGGG
Van91I 1839 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5671 CCATCTTGTGG
XcmI 2219 CCAGAGTCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4138 CCAGCGTTCAGATGG
XmaCI 315 CCCGGG C^CCGGG
1095 CCCGGG
XmaI 315 CCCGGG C^CCGGG
1095 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspI
AvaI AvaII BamHI BanI BanII
BbsI BbvI BcgI BcnI BfaI
BlpI BmyI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspHI BspMI BsrDI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
DrdI DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflFI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XbaI XhoII