##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AspI
AviII AvrII BanI BbeI BbrPI
BbvCI BcgI BciVI BfrI BlnI
BsaAI BsaHI BseAI BsiEI BsiWI
BspDI BspEI BsrBI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV FauI FseI FspI
HaeII HgaI Hin1I KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmlI
PpuMI PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SphI Sse8387I SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3375 GTAGAC GT^[AC][GT]AC
AclI 3603 AACGTT AA^CGTT
Asp700I 626 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1149 TGGCCA TGG^CCA
BamHI 1514 GGATCC G^GATCC
BglI 239 GCCCTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 5684 GCTAAGC GC^T[ACGT]AGC
BpmI 5372 CTGGAG CTGGAG(16/14)
Bpu1102I 5684 GCTAAGC GC^T[ACGT]AGC
BsaBI 4810 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 321 GGTCTC GGTCTC(1/5)
BsaWI 1299 ACCGGA [AT]^CCGG[AT]
BsmBI 1920 CGTCTC CGTCTC(1/5)
BsrDI 3202 CATTGC GCAATG(2/0)
BssSI 3042 CTCGTG CACGAG(-5/-1)
BstAPI 1597 GCAAATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 5684 GCTAAGC GC^T[ACGT]AGC
DraIII 3736 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1149 TGGCCA [CT]^GGCC[GA]
HindIII 4084 AAGCTT A^AGCTT
HpaI 3456 GTTAAC GTT^AAC
MamI 4810 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1149 TGGCCA TGG^CCA
MscI 1149 TGGCCA TGG^CCA
NheI 3650 GCTAGC G^CTAGC
PmeI 2462 GTTTAAAC GTTT^AAAC
PshAI 2227 GACATGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3603 AACGTT AA^CGTT
SacI 2769 GAGCTC GAGCT^C
SexAI 570 ACCTGGT A^CC[AT]GGT
SmaI 210 CCCGGG CCC^GGG
StuI 5368 AGGCCT AGG^CCT
XbaI 4026 TCTAGA T^CTAGA
XcmI 2089 CCATCTGCGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 210 CCCGGG C^CCGGG
XmaI 210 CCCGGG C^CCGGG
XmnI 626 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 4179 GTGCAC G^TGCAC
4437 GTGCAC
ApaLI 4179 GTGCAC G^TGCAC
4437 GTGCAC
AseI 3428 ATTAAT AT^TAAT
4922 ATTAAT
AsnI 3428 ATTAAT AT^TAAT
4922 ATTAAT
AvaI 31 CTCGGG C^[CT]CG[GA]G
210 CCCGGG
BglII 673 AGATCT A^GATCT
5154 AGATCT
BsgI 2169 CTGCAC GTGCAG(16/14)
2222 GTGCAG
BsoBI 31 CTCGGG C^[CT]CG[GA]G
210 CCCGGG
BspHI 3249 TCATGA T^CATGA
4914 TCATGA
CfoI 53 GCGC GCG^C
230 GCGC
EcoNI 2104 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5371 CCTGGAGAAGG
EcoRI 910 GAATTC G^AATTC
4932 GAATTC
EcoT14I 1459 CCAAGG C^C[AT][AT]GG
5569 CCAAGG
HhaI 53 GCGC GCG^C
230 GCGC
HinP1I 53 GCGC G^CGC
230 GCGC
PshBI 3428 ATTAAT AT^TAAT
4922 ATTAAT
PvuII 90 CAGCTG CAG^CTG
2335 CAGCTG
RcaI 3249 TCATGA T^CATGA
4914 TCATGA
SapI 2278 GAAGAGC GCTCTTC(1/4)
2374 GAAGAGC
SpeI 3565 ACTAGT A^CTAGT
3617 ACTAGT
StyI 1459 CCAAGG C^C[AT][AT]GG
5569 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbsI BbvI BclI BcnI
BfaI BmyI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspLU11I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT22I
FbaI Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII