##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BbeI BglII
BlnI BsaWI BseAI BsiEI BsiWI
BsmI Bsp1407I BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I Cfr10I
DraI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI FseI
FspI HincII HindII HpaI KasI
KpnI MluI MroI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RcaI SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccI 3428 GTCTAC GT^[AC][GT]AC
AcsI 4106 AAATTC [GA]^AATT[CT]
ApoI 4106 AAATTC [GA]^AATT[CT]
AspI 2787 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 4865 CACGTG CAC^GTG
BglI 1852 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 4865 CACGTG [CT]AC^GT[GA]
BsmBI 1688 CGTCTC CGTCTC(1/5)
BspDI 1565 ATCGAT AT^CGAT
BspMI 2701 GCAGGT ACCTGC(4/8)
BstAPI 4984 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 1565 ATCGAT AT^CGAT
CpoI 4590 CGGTCCG CG^G[AT]CCG
DraIII 3433 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 570 GATATC GAT^ATC
HaeII 5339 GGCGCT [GA]GCGC^[CT]
HindIII 1320 AAGCTT A^AGCTT
KspI 4480 CCGCGG CCGC^GG
MfeI 3466 CAATTG C^AATTG
MunI 3466 CAATTG C^AATTG
NdeI 1844 CATATG CA^TATG
PflFI 2787 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 4865 CACGTG CAC^GTG
PmlI 4865 CACGTG CAC^GTG
RsrII 4590 CGGTCCG CG^G[AT]CCG
SacII 4480 CCGCGG CCGC^GG
SapI 2940 GAAGAGC GCTCTTC(1/4)
ScaI 3876 AGTACT AGT^ACT
TaqI 1566 TCGA T^CGA
Tth111I 2787 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1566 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 4089 GACGCC G[GA]^CG[CT]C
4665 GGCGTC
AflII 1632 CTTAAG C^TTAAG
1749 CTTAAG
BamHI 3568 GGATCC G^GATCC
4693 GGATCC
BanI 2870 GGCACC G^G[CT][GA]CC
4705 GGCACC
BbvCI 3395 CCTCAGC CCTCAGC(-5/-2)
4210 CCTCAGC
BcgI 5183 GCAGCCGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5355 GCAGACCCCTCG
BclI 3654 TGATCA T^GATCA
4648 TGATCA
BfrI 1632 CTTAAG C^TTAAG
1749 CTTAAG
BsaHI 4089 GACGCC G[GA]^CG[CT]C
4665 GGCGTC
BspLU11I 2110 ACATGT A^CATGT
3968 ACATGT
BstEII 79 GGTCACC G^GT[ACGT]ACC
180 GGTAACC
BstPI 79 GGTCACC G^GT[ACGT]ACC
180 GGTAACC
DrdI 18 GACTAGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4790 GACCATCCAGTC
EarI 3683 CTCTTC CTCTTC(1/4)
2940 GAAGAG
EcoO65I 79 GGTCACC G^GT[ACGT]ACC
180 GGTAACC
FbaI 3654 TGATCA T^GATCA
4648 TGATCA
Hin1I 4089 GACGCC G[GA]^CG[CT]C
4665 GGCGTC
Ksp632I 3683 CTCTTC CTCTTC(1/4)
2940 GAAGAG
NheI 1716 GCTAGC G^CTAGC
3163 GCTAGC
PflMI 84 CCAATCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1371 CCAATCTGTGG
PshAI 73 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2266 GACCAGGGTC
PvuII 3861 CAGCTG CAG^CTG
5160 CAGCTG
SacI 1520 GAGCTC GAGCT^C
5003 GAGCTC
StuI 2477 AGGCCT AGG^CCT
4207 AGGCCT
Van91I 84 CCAATCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1371 CCAATCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanII BbsI BbvI
BciVI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII XmaCI XmaI