##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
ApaLI AscI Asp718I AspEI AviII
BbeI BfrI BsaHI BseAI BsiWI
BspDI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraIII Eam1105I EcoO65I
EcoRV EcoT22I FseI FspI Hin1I
KasI KpnI MfeI MluI MroI
MunI NarI NdeI NotI NruI
NsiI PacI PinAI PshAI Psp1406I
PvuI RcaI RsrII SalI SbfI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AocI 5074 CCTTAGG CC^T[ACGT]AGG
Aor51HI 3194 AGCGCT AGC^GCT
AvrII 4926 CCTAGG C^CTAGG
BalI 4355 TGGCCA TGG^CCA
BamHI 5229 GGATCC G^GATCC
BbrPI 1975 CACGTG CAC^GTG
BcgI 2971 CGAGCACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3055 GTATCC GTATCC(6/5)
BglI 2658 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 975 AGATCT A^GATCT
BlnI 4926 CCTAGG C^CTAGG
BsaWI 1040 ACCGGA [AT]^CCGG[AT]
BsmBI 4505 CGTCTC CGTCTC(1/5)
Bsp1407I 5723 TGTACA T^GTACA
BsrGI 5723 TGTACA T^GTACA
BstBI 4412 TTCGAA TT^CGAA
BstXI 3827 CCAGAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 5074 CCTTAGG CC^T[ACGT]AGG
DrdI 1156 GACAGGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3194 AGCGCT AGC^GCT
Eco81I 5074 CCTTAGG CC^T[ACGT]AGG
EcoNI 2298 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 2913 AAGCTT A^AGCTT
HpaI 5573 GTTAAC GTT^AAC
KspI 134 CCGCGG CCGC^GG
MluNI 4355 TGGCCA TGG^CCA
MscI 4355 TGGCCA TGG^CCA
NaeI 4886 GCCGGC GCC^GGC
NgoMIV 4886 GCCGGC G^CCGGC
NspV 4412 TTCGAA TT^CGAA
PaeR7I 2969 CTCGAG C^TCGAG
PmaCI 1975 CACGTG CAC^GTG
PmeI 6182 GTTTAAAC GTTT^AAAC
PmlI 1975 CACGTG CAC^GTG
SacI 2787 GAGCTC GAGCT^C
SacII 134 CCGCGG CCGC^GG
SapI 4699 GAAGAGC GCTCTTC(1/4)
ScaI 3774 AGTACT AGT^ACT
SexAI 4256 ACCAGGT A^CC[AT]GGT
SfuI 4412 TTCGAA TT^CGAA
SspBI 5723 TGTACA T^GTACA
XhoI 2969 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 57 CGCG CG^CG
135 CGCG
AflIII 1976 ACGTGT A^C[GA][CT]GT
5716 ACGTGT
ApaI 2396 GGGCCC GGGCC^C
2688 GGGCCC
AseI 4848 ATTAAT AT^TAAT
6403 ATTAAT
AsnI 4848 ATTAAT AT^TAAT
6403 ATTAAT
AspI 2314 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2373 GACAGAGTC
AvaI 2969 CTCGAG C^[CT]CG[GA]G
4077 CCCGAG
BbvCI 1618 CCTCAGC CCTCAGC(-5/-2)
3350 GCTGAGG
BsaAI 1975 CACGTG [CT]AC^GT[GA]
5715 TACGTG
BsaBI 1674 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6402 GATTAATATC
BsmI 389 GAATGC GAATGC(1/-1)
1648 GCATTC
BsoBI 2969 CTCGAG C^[CT]CG[GA]G
4077 CCCGAG
BsrBI 6322 CCGCTC CCGCTC(-3/-3)
1849 GAGCGG
BsrFI 34 ACCGGC [GA]^CCGG[CT]
4886 GCCGGC
BstUI 57 CGCG CG^CG
135 CGCG
Cfr10I 34 ACCGGC [GA]^CCGG[CT]
4886 GCCGGC
HgaI 4504 GCGTC GACGC(5/10)
4594 GCGTC
HincII 4574 GTTGAC GT[CT]^[GA]AC
5573 GTTAAC
HindII 4574 GTTGAC GT[CT]^[GA]AC
5573 GTTAAC
MamI 1674 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
6402 GATTAATATC
MvnI 57 CGCG CG^CG
135 CGCG
NheI 3900 GCTAGC G^CTAGC
4326 GCTAGC
PflFI 2314 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2373 GACAGAGTC
PflMI 2292 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2304 CCAGGGGGTGG
PshBI 4848 ATTAAT AT^TAAT
6403 ATTAAT
StuI 1468 AGGCCT AGG^CCT
5825 AGGCCT
Tth111I 2314 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2373 GACAGAGTC
Van91I 2292 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2304 CCAGGGGGTGG
XcmI 2401 CCACACTGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3568 CCAAACCTGCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BclI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI SphI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI