##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccIII AclI AscI
Asp718I AviII AvrII BamHI BbrPI
BclI BlnI BlpI Bpu1102I BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I CelII ClaI CpoI EcoRV
FbaI FseI FspI KpnI KspI
MluI MroI NaeI NgoMIV NheI
NotI NruI NspV PacI PmaCI
PmlI PshAI Psp1406I RsrII SacII
SalI SbfI SfiI SfuI SmaI
SnaBI Sse8387I SspBI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 5435 GTCTAC GT^[AC][GT]AC
AccII 5180 CGCG CG^CG
AcyI 6071 GGCGCC G[GA]^CG[CT]C
AflII 5682 CTTAAG C^TTAAG
AflIII 2288 ACGTGT A^C[GA][CT]GT
AgeI 508 ACCGGT A^CCGGT
AhdI 1781 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 5823 AGCGCT AGC^GCT
AspEI 1781 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 6071 GGCGCC G^G[CT][GA]CC
BbeI 6071 GGCGCC GGCGC^C
BcgI 6393 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 5682 CTTAAG C^TTAAG
BglI 423 GCCCTGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2287 TACGTG [CT]AC^GT[GA]
BsaBI 1255 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 6071 GGCGCC G[GA]^CG[CT]C
BsmBI 1882 GAGACG CGTCTC(1/5)
BsrBI 1464 GAGCGG CCGCTC(-3/-3)
BssSI 441 CACGAG CACGAG(-5/-1)
BstEII 145 GGTCACC G^GT[ACGT]ACC
BstPI 145 GGTCACC G^GT[ACGT]ACC
BstUI 5180 CGCG CG^CG
DraIII 1498 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1807 GACTTTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 73 CGGCCG C^GGCCG
Eam1105I 1781 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 73 CGGCCG C^GGCCG
Eco47III 5823 AGCGCT AGC^GCT
Eco52I 73 CGGCCG C^GGCCG
EcoO65I 145 GGTCACC G^GT[ACGT]ACC
Hin1I 6071 GGCGCC G[GA]^CG[CT]C
HpaI 2614 GTTAAC GTT^AAC
KasI 6071 GGCGCC G^GCGCC
MamI 1255 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 289 CAATTG C^AATTG
MunI 289 CAATTG C^AATTG
MvnI 5180 CGCG CG^CG
NarI 6071 GGCGCC GG^CGCC
PaeR7I 2304 CTCGAG C^TCGAG
PinAI 508 ACCGGT A^CCGGT
PmeI 5890 GTTTAAAC GTTT^AAAC
PpuMI 1456 AGGACCC [GA]G^G[AT]CC[CT]
PvuI 1207 CGATCG CGAT^CG
SexAI 5615 ACCAGGT A^CC[AT]GGT
SfoI 6071 GGCGCC GGC^GCC
SpeI 4727 ACTAGT A^CTAGT
StuI 1406 AGGCCT AGG^CCT
XbaI 3410 TCTAGA T^CTAGA
XhoI 2304 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
ApaI 1187 GGGCCC GGGCC^C
1749 GGGCCC
AseI 692 ATTAAT AT^TAAT
1025 ATTAAT
AsnI 692 ATTAAT AT^TAAT
1025 ATTAAT
Asp700I 1224 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3110 GAAATTGTTC
AspI 5614 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6190 GACTTTGTC
AvaI 2304 CTCGAG C^[CT]CG[GA]G
4241 CCCGAG
BglII 1156 AGATCT A^GATCT
5656 AGATCT
BsaWI 149 ACCGGA [AT]^CCGG[AT]
508 ACCGGT
BsiEI 73 CGGCCG CG[GA][CT]^CG
1207 CGATCG
BsoBI 2304 CTCGAG C^[CT]CG[GA]G
4241 CCCGAG
BspMI 251 ACCTGC ACCTGC(4/8)
3971 GCAGGT
BsrDI 6447 CATTGC GCAATG(2/0)
5647 GCAATG
CfoI 5824 GCGC GCG^C
6072 GCGC
EcoNI 4904 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5447 CCTCAAAGAGG
FauI 1359 CCCGC CCCGC(4/6)
5832 CCCGC
HaeII 5823 AGCGCT [GA]GCGC^[CT]
6071 GGCGCC
HgaI 1528 GCGTC GACGC(5/10)
5181 GCGTC
HhaI 5824 GCGC GCG^C
6072 GCGC
HinP1I 5824 GCGC G^CGC
6072 GCGC
HincII 2614 GTTAAC GT[CT]^[GA]AC
3359 GTTGAC
HindII 2614 GTTAAC GT[CT]^[GA]AC
3359 GTTGAC
NcoI 1590 CCATGG C^CATGG
4488 CCATGG
NdeI 3186 CATATG CA^TATG
4372 CATATG
PflFI 5614 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6190 GACTTTGTC
PshBI 692 ATTAAT AT^TAAT
1025 ATTAAT
SacI 4054 GAGCTC GAGCT^C
5816 GAGCTC
SapI 2885 GAAGAGC GCTCTTC(1/4)
3326 GAAGAGC
SgrAI 130 CACCGGCG C[GA]^CCGG[CT]G
155 CACCGGCG
Tth111I 5614 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
6190 GACTTTGTC
XmnI 1224 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3110 GAAATTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI Alw44I
AlwI AlwNI AocI ApaLI ApoI
AspHI AvaII BalI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SphI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII