##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AclI AflII AocI AscI AseI
AsnI AvrII BamHI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsiWI BspDI BspHI BspLU11I
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eco81I EcoRV
EcoT22I FbaI FseI HpaI MamI
MluI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshBI Psp1406I PvuI RcaI RsrII
SfiI SfuI SnaBI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 525 GACGTC GACGT^C
Acc65I 3465 GGTACC G^GTACC
AccIII 1892 TCCGGA T^CCGGA
AflIII 786 ACACGT A^C[GA][CT]GT
AgeI 701 ACCGGT A^CCGGT
AhdI 1997 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 95 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3465 GGTACC G^GTACC
AspEI 1997 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3059 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2613 TGCGCA TGC^GCA
BbvCI 1864 GCTGAGG CCTCAGC(-5/-2)
BcgI 3373 CGAGGTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 4195 GGTCTC GGTCTC(1/5)
BseAI 1892 TCCGGA T^CCGGA
BspEI 1892 TCCGGA T^CCGGA
BsrDI 1245 GCAATG GCAATG(2/0)
DraIII 826 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1997 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 29 GAATTC G^AATTC
FspI 2613 TGCGCA TGC^GCA
HincII 599 GTCGAC GT[CT]^[GA]AC
HindII 599 GTCGAC GT[CT]^[GA]AC
HindIII 431 AAGCTT A^AGCTT
KpnI 3465 GGTACC GGTAC^C
MfeI 4832 CAATTG C^AATTG
MroI 1892 TCCGGA T^CCGGA
MunI 4832 CAATTG C^AATTG
PflFI 3059 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 701 ACCGGT A^CCGGT
PshAI 525 GACGTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 599 GTCGAC G^TCGAC
SexAI 693 ACCTGGT A^CC[AT]GGT
SgrAI 700 CACCGGTG C[GA]^CCGG[CT]G
SmlI 1094 CTTGAG C^T[CT][GA]AG
SpeI 97 ACTAGT A^CTAGT
Tth111I 3059 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 966 CCAGCTCCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 95 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 599 GTCGAC GT^[AC][GT]AC
1754 GTCTAC
Alw44I 3557 GTGCAC G^TGCAC
5039 GTGCAC
Aor51HI 1698 AGCGCT AGC^GCT
3261 AGCGCT
ApaLI 3557 GTGCAC G^TGCAC
5039 GTGCAC
BfaI 98 CTAG C^TAG
2432 CTAG
BsaWI 701 ACCGGT [AT]^CCGG[AT]
1892 TCCGGA
BsmI 3379 GAATGC GAATGC(1/-1)
4432 GCATTC
Bsp1407I 2814 TGTACA T^GTACA
4801 TGTACA
BsrGI 2814 TGTACA T^GTACA
4801 TGTACA
BssSI 3497 CACGAG CACGAG(-5/-1)
5025 CACGAG
DrdI 3431 GACCCCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3713 GACCCGCGCGTC
Eco47III 1698 AGCGCT AGC^GCT
3261 AGCGCT
MaeI 98 CTAG C^TAG
2432 CTAG
SbfI 3610 CCTGCAGG CCTGCA^GG
4720 CCTGCAGG
ScaI 203 AGTACT AGT^ACT
3105 AGTACT
Sse8387I 3610 CCTGCAGG CCTGCA^GG
4720 CCTGCAGG
SspBI 2814 TGTACA T^GTACA
4801 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 145 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SacII SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmaCI XmaI