################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## Acc65I AclI AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BbsI BciVI BclI BglII BpuAI BsaBI BsiWI BsmI BspDI BspHI Bst1107I BstBI BstZ17I ClaI CpoI DrdI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 840 GACGTC GACGT^C AccI 97 GTCGAC GT^[AC][GT]AC AccIII 160 TCCGGA T^CCGGA AcsI 4803 AAATTT [GA]^AATT[CT] AflII 4510 CTTAAG C^TTAAG Aor51HI 1307 AGCGCT AGC^GCT ApoI 4803 AAATTT [GA]^AATT[CT] Asp700I 4464 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 3647 CCTAGG C^CTAGG BcgI 3183 CGATGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 4510 CTTAAG C^TTAAG BlnI 3647 CCTAGG C^CTAGG BlpI 1158 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1158 GCTGAGC GC^T[ACGT]AGC BseAI 160 TCCGGA T^CCGGA Bsp1407I 1519 TGTACA T^GTACA BspEI 160 TCCGGA T^CCGGA BspLU11I 4206 ACATGT A^CATGT BsrDI 4261 CATTGC GCAATG(2/0) BsrGI 1519 TGTACA T^GTACA BstEII 2239 GGTCACC G^GT[ACGT]ACC BstPI 2239 GGTCACC G^GT[ACGT]ACC CelII 1158 GCTGAGC GC^T[ACGT]AGC Eco47III 1307 AGCGCT AGC^GCT EcoO65I 2239 GGTCACC G^GT[ACGT]ACC HincII 97 GTCGAC GT[CT]^[GA]AC HindII 97 GTCGAC GT[CT]^[GA]AC MluI 1870 ACGCGT A^CGCGT MroI 160 TCCGGA T^CCGGA PaeR7I 3174 CTCGAG C^TCGAG PshAI 2992 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 3498 GAGCTC GAGCT^C SalI 97 GTCGAC G^TCGAC SspBI 1519 TGTACA T^GTACA TfiI 493 GATTC G^A[AT]TC XhoI 3174 CTCGAG C^TCGAG XmnI 4464 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AocI 2234 CCTGAGG CC^T[ACGT]AGG 2676 CCTGAGG BbeI 203 GGCGCC GGCGC^C 2181 GGCGCC BsmBI 708 GAGACG CGTCTC(1/5) 1922 GAGACG BssSI 1715 CACGAG CACGAG(-5/-1) 3800 CTCGTG BstYI 788 GGATCT [GA]^GATC[CT] 4425 GGATCT Bsu36I 2234 CCTGAGG CC^T[ACGT]AGG 2676 CCTGAGG DraI 4795 TTTAAA TTT^AAA 4807 TTTAAA Eco81I 2234 CCTGAGG CC^T[ACGT]AGG 2676 CCTGAGG KasI 203 GGCGCC G^GCGCC 2181 GGCGCC MflI 788 GGATCT [GA]^GATC[CT] 4425 GGATCT NarI 203 GGCGCC GG^CGCC 2181 GGCGCC NspI 1507 ACATGC [GA]CATG^[CT] 4206 ACATGT SapI 365 GCTCTTC GCTCTTC(1/4) 3300 GCTCTTC ScaI 1033 AGTACT AGT^ACT 1063 AGTACT SfoI 203 GGCGCC GGC^GCC 2181 GGCGCC SmaI 122 CCCGGG CCC^GGG 2448 CCCGGG SmlI 3174 CTCGAG C^T[CT][GA]AG 4510 CTTAAG XhoII 788 GGATCT [GA]^GATC[CT] 4425 GGATCT XmaCI 122 CCCGGG C^CCGGG 2448 CCCGGG XmaI 122 CCCGGG C^CCGGG 2448 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 130 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbrPI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI