##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

Acc65I      AclI        AgeI        AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       BamHI       BbsI        
BciVI       BclI        BglII       BpuAI       BsaBI       
BsiWI       BsmI        BspDI       BspHI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DrdI        
Eam1105I    EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
MamI        MfeI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmeI        PshBI       Psp1406I    
PvuI        RcaI        RsrII       SbfI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII         840  GACGTC               GACGT^C
AccI           97  GTCGAC               GT^[AC][GT]AC
AccIII        160  TCCGGA               T^CCGGA
AcsI         4803  AAATTT               [GA]^AATT[CT]
AflII        4510  CTTAAG               C^TTAAG
Aor51HI      1307  AGCGCT               AGC^GCT
ApoI         4803  AAATTT               [GA]^AATT[CT]
Asp700I      4464  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        3647  CCTAGG               C^CTAGG
BcgI         3183  CGATGCCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         4510  CTTAAG               C^TTAAG
BlnI         3647  CCTAGG               C^CTAGG
BlpI         1158  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1158  GCTGAGC              GC^T[ACGT]AGC
BseAI         160  TCCGGA               T^CCGGA
Bsp1407I     1519  TGTACA               T^GTACA
BspEI         160  TCCGGA               T^CCGGA
BspLU11I     4206  ACATGT               A^CATGT
BsrDI        4261  CATTGC               GCAATG(2/0)
BsrGI        1519  TGTACA               T^GTACA
BstEII       2239  GGTCACC              G^GT[ACGT]ACC
BstPI        2239  GGTCACC              G^GT[ACGT]ACC
CelII        1158  GCTGAGC              GC^T[ACGT]AGC
Eco47III     1307  AGCGCT               AGC^GCT
EcoO65I      2239  GGTCACC              G^GT[ACGT]ACC
HincII         97  GTCGAC               GT[CT]^[GA]AC
HindII         97  GTCGAC               GT[CT]^[GA]AC
MluI         1870  ACGCGT               A^CGCGT
MroI          160  TCCGGA               T^CCGGA
PaeR7I       3174  CTCGAG               C^TCGAG
PshAI        2992  GACTGAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI         3498  GAGCTC               GAGCT^C
SalI           97  GTCGAC               G^TCGAC
SspBI        1519  TGTACA               T^GTACA
TfiI          493  GATTC                G^A[AT]TC
XhoI         3174  CTCGAG               C^TCGAG
XmnI         4464  GAACCACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AocI         2234  CCTGAGG              CC^T[ACGT]AGG
             2676  CCTGAGG             
BbeI          203  GGCGCC               GGCGC^C
             2181  GGCGCC              
BsmBI         708  GAGACG               CGTCTC(1/5)
             1922  GAGACG              
BssSI        1715  CACGAG               CACGAG(-5/-1)
             3800  CTCGTG              
BstYI         788  GGATCT               [GA]^GATC[CT]
             4425  GGATCT              
Bsu36I       2234  CCTGAGG              CC^T[ACGT]AGG
             2676  CCTGAGG             
DraI         4795  TTTAAA               TTT^AAA
             4807  TTTAAA              
Eco81I       2234  CCTGAGG              CC^T[ACGT]AGG
             2676  CCTGAGG             
KasI          203  GGCGCC               G^GCGCC
             2181  GGCGCC              
MflI          788  GGATCT               [GA]^GATC[CT]
             4425  GGATCT              
NarI          203  GGCGCC               GG^CGCC
             2181  GGCGCC              
NspI         1507  ACATGC               [GA]CATG^[CT]
             4206  ACATGT              
SapI          365  GCTCTTC              GCTCTTC(1/4)
             3300  GCTCTTC             
ScaI         1033  AGTACT               AGT^ACT
             1063  AGTACT              
SfoI          203  GGCGCC               GGC^GCC
             2181  GGCGCC              
SmaI          122  CCCGGG               CCC^GGG
             2448  CCCGGG              
SmlI         3174  CTCGAG               C^T[CT][GA]AG
             4510  CTTAAG              
XhoII         788  GGATCT               [GA]^GATC[CT]
             4425  GGATCT              
XmaCI         122  CCCGGG               C^CCGGG
             2448  CCCGGG              
XmaI          122  CCCGGG               C^CCGGG
             2448  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  130 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbrPI       BbvCI       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaAI       BsaHI       
BsaI        BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssHII      BssKI       BstAPI      
BstF5I      BstNI       BstUI       BstXI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DraIII      DsaI        EaeI        EagI        
EarI        EclXI       Eco52I      EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
PflMI       PleI        PmaCI       PmlI        PpuMI       
PstI        PvuII       RsaI        SacII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StuI        
StyI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     Van91I      XcmI