##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI Alw44I Aor51HI ApaLI
AscI Asp700I Asp718I AviII AvrII
BanI BbeI BbrPI BcgI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrFI
BssHII BstEII BstPI BstUI BstXI
CelII CfoI Cfr10I ClaI CpoI
EagI EclXI Eco47III Eco52I EcoO65I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI PacI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SgrAI SnaBI
Sse8387I StuI SwaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 129 GTATAC GT^[AC][GT]AC
AclI 3182 AACGTT AA^CGTT
AhdI 184 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3098 CCTGAGG CC^T[ACGT]AGG
ApaI 1254 GGGCCC GGGCC^C
AseI 814 ATTAAT AT^TAAT
AsnI 814 ATTAAT AT^TAAT
AspEI 184 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 441 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2075 GGATCC G^GATCC
BsgI 660 GTGCAG GTGCAG(16/14)
Bsp1407I 3195 TGTACA T^GTACA
BspHI 563 TCATGA T^CATGA
BsrBI 2571 CCGCTC CCGCTC(-3/-3)
BsrGI 3195 TGTACA T^GTACA
BssSI 272 CACGAG CACGAG(-5/-1)
Bst1107I 129 GTATAC GTA^TAC
BstAPI 2179 GCAGGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2590 TTCGAA TT^CGAA
BstZ17I 129 GTATAC GTA^TAC
Bsu36I 3098 CCTGAGG CC^T[ACGT]AGG
DraIII 3412 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 86 GACCTCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 184 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3098 CCTGAGG CC^T[ACGT]AGG
EcoNI 3401 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 559 GATATC GAT^ATC
EcoT22I 229 ATGCAT ATGCA^T
HapII 2583 CCGG C^CGG
HincII 203 GTCAAC GT[CT]^[GA]AC
HindII 203 GTCAAC GT[CT]^[GA]AC
HindIII 329 AAGCTT A^AGCTT
HpaII 2583 CCGG C^CGG
MspI 2583 CCGG C^CGG
NdeI 1907 CATATG CA^TATG
NsiI 229 ATGCAT ATGCA^T
NspV 2590 TTCGAA TT^CGAA
PaeR7I 919 CTCGAG C^TCGAG
PflFI 441 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3625 CCATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 814 ATTAAT AT^TAAT
Psp1406I 3182 AACGTT AA^CGTT
PstI 4 CTGCAG CTGCA^G
RcaI 563 TCATGA T^CATGA
SfuI 2590 TTCGAA TT^CGAA
SmaI 2582 CCCGGG CCC^GGG
SmlI 919 CTCGAG C^T[CT][GA]AG
SpeI 3843 ACTAGT A^CTAGT
SphI 459 GCATGC GCATG^C
SspBI 3195 TGTACA T^GTACA
SspI 1762 AATATT AAT^ATT
Tsp45I 3018 GTCAC ^GT[GC]AC
Tth111I 441 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3625 CCATGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3604 TCTAGA T^CTAGA
XhoI 919 CTCGAG C^TCGAG
XmaCI 2582 CCCGGG C^CCGGG
XmaI 2582 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 919 CTCGAG C^[CT]CG[GA]G
2582 CCCGGG
BcnI 2582 CCCGG CC^[GC]GG
2583 CCGGG
BfaI 3605 CTAG C^TAG
3844 CTAG
BglII 264 AGATCT A^GATCT
708 AGATCT
BsaBI 2076 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3121 GATGAAAATC
BsaI 439 GAGACC GGTCTC(1/5)
510 GAGACC
BseRI 536 CTCCTC GAGGAG(10/8)
1242 CTCCTC
BsmI 2643 GAATGC GAATGC(1/-1)
1188 GCATTC
BsoBI 919 CTCGAG C^[CT]CG[GA]G
2582 CCCGGG
BspLU11I 143 ACATGT A^CATGT
3668 ACATGT
DsaI 1913 CCATGG C^C[GA][CT]GG
2071 CCATGG
EcoRI 1378 GAATTC G^AATTC
3600 GAATTC
MaeI 3605 CTAG C^TAG
3844 CTAG
MamI 2076 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3121 GATGAAAATC
NciI 2582 CCCGG CC^[GC]GG
2583 CCGGG
NcoI 1913 CCATGG C^CATGG
2071 CCATGG
PpuMI 281 GGGACCT [GA]G^G[AT]CC[CT]
4044 AGGACCC
SacI 2156 GAGCTC GAGCT^C
3916 GAGCTC
SexAI 1160 ACCTGGT A^CC[AT]GGT
2246 ACCAGGT
TaqI 920 TCGA T^CGA
2591 TCGA
TthHB8I 920 TCGA T^CGA
2591 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanII BbsI BbvCI BbvI
BclI BmyI BpmI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp509I TspRI XhoII