##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AcyI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AvaI AviII AvrII BanI
BbeI BcgI BciVI BfrI BglI
BlnI BsaHI BsiWI BsmBI BsmI
BsoBI BspDI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CfoI ClaI CpoI DraI DraIII
DrdI Eco47III EcoO65I EcoRI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I KasI KpnI KspI MluI
NarI NdeI NheI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PvuI RsrII SacII SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI StuI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 4227 CGCG CG^CG
AclI 3787 AACGTT AA^CGTT
AcsI 3775 AAATTC [GA]^AATT[CT]
AhdI 3390 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 652 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 3775 AAATTC [GA]^AATT[CT]
AseI 4038 ATTAAT AT^TAAT
AsnI 4038 ATTAAT AT^TAAT
AspEI 3390 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3168 GGATCC G^GATCC
BbrPI 2760 CACGTG CAC^GTG
BglII 3925 AGATCT A^GATCT
BsaBI 4008 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3323 GAGACC GGTCTC(1/5)
BsiEI 4233 CGGCCG CG[GA][CT]^CG
Bsp1407I 1445 TGTACA T^GTACA
BspLU11I 2324 ACATGT A^CATGT
BspMI 3036 ACCTGC ACCTGC(4/8)
BsrBI 236 CCGCTC CCGCTC(-3/-3)
BsrGI 1445 TGTACA T^GTACA
BstUI 4227 CGCG CG^CG
BstXI 2849 CCATGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 1331 CCATGG C^C[GA][CT]GG
EagI 4233 CGGCCG C^GGCCG
Eam1105I 3390 GACAGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 4233 CGGCCG C^GGCCG
Eco52I 4233 CGGCCG C^GGCCG
EcoRV 3886 GATATC GAT^ATC
EcoT22I 2308 ATGCAT ATGCA^T
HpaI 3220 GTTAAC GTT^AAC
MamI 4008 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 4227 CGCG CG^CG
NaeI 702 GCCGGC GCC^GGC
NcoI 1331 CCATGG C^CATGG
NgoMIV 702 GCCGGC G^CCGGC
NotI 4232 GCGGCCGC GC^GGCCGC
NsiI 2308 ATGCAT ATGCA^T
PmaCI 2760 CACGTG CAC^GTG
PmlI 2760 CACGTG CAC^GTG
PshBI 4038 ATTAAT AT^TAAT
Psp1406I 3787 AACGTT AA^CGTT
SalI 1395 GTCGAC G^TCGAC
SapI 2490 GAAGAGC GCTCTTC(1/4)
SbfI 2739 CCTGCAGG CCTGCA^GG
SexAI 2671 ACCTGGT A^CC[AT]GGT
Sse8387I 2739 CCTGCAGG CCTGCA^GG
SspBI 1445 TGTACA T^GTACA
SspI 3650 AATATT AAT^ATT
XcmI 2801 CCAGACATAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 272 TCCGGA T^CCGGA
1085 TCCGGA
AflIII 2324 ACATGT A^C[GA][CT]GT
2905 ACACGT
Asp700I 390 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1203 GAAGATGTTC
BalI 28 TGGCCA TGG^CCA
841 TGGCCA
BclI 2940 TGATCA T^GATCA
4010 TGATCA
BcnI 2933 CCGGG CC^[GC]GG
3796 CCGGG
BsaAI 2760 CACGTG [CT]AC^GT[GA]
2967 CACGTA
BsaWI 272 TCCGGA [AT]^CCGG[AT]
1085 TCCGGA
BseAI 272 TCCGGA T^CCGGA
1085 TCCGGA
BspEI 272 TCCGGA T^CCGGA
1085 TCCGGA
BsrFI 702 GCCGGC [GA]^CCGG[CT]
3413 GCCGGT
BstAPI 2860 GCAACCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2923 GCAGCACATGC
BstYI 3168 GGATCC [GA]^GATC[CT]
3925 AGATCT
Cfr10I 702 GCCGGC [GA]^CCGG[CT]
3413 GCCGGT
EcoNI 2049 CCTGACTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3037 CCTGCTCAAGG
FbaI 2940 TGATCA T^GATCA
4010 TGATCA
MfeI 3215 CAATTG C^AATTG
3270 CAATTG
MflI 3168 GGATCC [GA]^GATC[CT]
3925 AGATCT
MluNI 28 TGGCCA TGG^CCA
841 TGGCCA
MroI 272 TCCGGA T^CCGGA
1085 TCCGGA
MscI 28 TGGCCA TGG^CCA
841 TGGCCA
MunI 3215 CAATTG C^AATTG
3270 CAATTG
NciI 2933 CCGGG CC^[GC]GG
3796 CCGGG
PflMI 651 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1916 CCAAGAAGTGG
SacI 147 GAGCTC GAGCT^C
960 GAGCTC
ScaI 419 AGTACT AGT^ACT
3281 AGTACT
Van91I 651 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1916 CCAAGAAGTGG
XbaI 740 TCTAGA T^CTAGA
4220 TCTAGA
XhoII 3168 GGATCC [GA]^GATC[CT]
3925 AGATCT
XmnI 390 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1203 GAAGATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AocI AspHI AspI AvaII BanII
BbsI BbvCI BbvI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
CelII Cfr13I DdeI DpnII DraII
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflFI PleI PpuMI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I