##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AclI AflII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
AspEI AviII AvrII BbrPI BciVI
BfrI BlnI BsaAI BsiEI BsiWI
BspDI BspHI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoT22I FseI FspI HpaI
KspI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SfiI SfuI SnaBI
SphI StuI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 245 GACGTC GACGT^C
Acc65I 1969 GGTACC G^GTACC
AccI 2593 GTCTAC GT^[AC][GT]AC
AccIII 1399 TCCGGA T^CCGGA
AflIII 4081 ACATGT A^C[GA][CT]GT
AocI 1946 CCTCAGG CC^T[ACGT]AGG
Asp718I 1969 GGTACC G^GTACC
BbeI 3208 GGCGCC GGCGC^C
BbvCI 803 GCTGAGG CCTCAGC(-5/-2)
BcgI 2455 CGAGCTCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 893 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 577 AGATCT A^GATCT
BsaBI 2275 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 380 GAGACC GGTCTC(1/5)
BseAI 1399 TCCGGA T^CCGGA
BsmBI 1219 CGTCTC CGTCTC(1/5)
BspEI 1399 TCCGGA T^CCGGA
BspLU11I 4081 ACATGT A^CATGT
BsrFI 1660 GCCGGT [GA]^CCGG[CT]
BstAPI 487 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1946 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1660 GCCGGT [GA]^CCGG[CT]
EarI 3532 GAAGAG CTCTTC(1/4)
Eco81I 1946 CCTCAGG CC^T[ACGT]AGG
EcoRI 2078 GAATTC G^AATTC
EcoRV 1480 GATATC GAT^ATC
HindIII 2850 AAGCTT A^AGCTT
KasI 3208 GGCGCC G^GCGCC
KpnI 1969 GGTACC GGTAC^C
Ksp632I 3532 GAAGAG CTCTTC(1/4)
MamI 2275 GATTAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2874 CAATTG C^AATTG
MroI 1399 TCCGGA T^CCGGA
MunI 2874 CAATTG C^AATTG
NarI 3208 GGCGCC GG^CGCC
PaeR7I 2660 CTCGAG C^TCGAG
PshAI 1790 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 3532 GAAGAGC GCTCTTC(1/4)
SbfI 1489 CCTGCAGG CCTGCA^GG
ScaI 51 AGTACT AGT^ACT
SexAI 2783 ACCAGGT A^CC[AT]GGT
SfoI 3208 GGCGCC GGC^GCC
SgrAI 1659 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 3498 CCCGGG CCC^GGG
SpeI 3362 ACTAGT A^CTAGT
Sse8387I 1489 CCTGCAGG CCTGCA^GG
SspI 2240 AATATT AAT^ATT
XhoI 2660 CTCGAG C^TCGAG
XmaCI 3498 CCCGGG C^CCGGG
XmaI 3498 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 3250 CGCG CG^CG
3319 CGCG
Alw44I 2690 GTGCAC G^TGCAC
2719 GTGCAC
ApaLI 2690 GTGCAC G^TGCAC
2719 GTGCAC
AvaI 2660 CTCGAG C^[CT]CG[GA]G
3498 CCCGGG
BamHI 1003 GGATCC G^GATCC
3356 GGATCC
BclI 2806 TGATCA T^GATCA
3171 TGATCA
BsmI 1884 GCATTC GAATGC(1/-1)
2483 GCATTC
BsoBI 2660 CTCGAG C^[CT]CG[GA]G
3498 CCCGGG
Bsp1407I 4008 TGTACA T^GTACA
4084 TGTACA
BsrBI 701 GAGCGG CCGCTC(-3/-3)
3535 GAGCGG
BsrDI 274 CATTGC GCAATG(2/0)
188 GCAATG
BsrGI 4008 TGTACA T^GTACA
4084 TGTACA
BstUI 3250 CGCG CG^CG
3319 CGCG
DraI 2902 TTTAAA TTT^AAA
2956 TTTAAA
DrdI 1769 GACATTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3431 GACCGCATCGTC
FbaI 2806 TGATCA T^GATCA
3171 TGATCA
HincII 98 GTTGAC GT[CT]^[GA]AC
2798 GTTGAC
HindII 98 GTTGAC GT[CT]^[GA]AC
2798 GTTGAC
MvnI 3250 CGCG CG^CG
3319 CGCG
NcoI 2724 CCATGG C^CATGG
3740 CCATGG
NspI 1371 ACATGC [GA]CATG^[CT]
4081 ACATGT
PflMI 1699 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1732 CCAAGACATGG
SspBI 4008 TGTACA T^GTACA
4084 TGTACA
TaqI 2328 TCGA T^CGA
2661 TCGA
TthHB8I 2328 TCGA T^CGA
2661 TCGA
Van91I 1699 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1732 CCAAGACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApoI AspHI AspI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BssSI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflFI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
XcmI XhoII