##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
Acc65I AccI AccIII ApaI AscI
Asp718I AspI AvrII BbeI BbrPI
BcgI BlnI BseAI BsiEI BsiWI
BspEI BssHII Bst1107I BstBI BstZ17I
CpoI DraIII DrdI EagI EclXI
Eco52I FseI HpaI KasI KpnI
KspI MluI MroI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PflFI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 5738 GACGTC GACGT^C
AccII 2788 CGCG CG^CG
AclI 1990 AACGTT AA^CGTT
AcyI 5738 GACGTC G[GA]^CG[CT]C
AflII 3451 CTTAAG C^TTAAG
AgeI 5780 ACCGGT A^CCGGT
AhdI 3142 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 707 GTGCAC G^TGCAC
Aor51HI 2021 AGCGCT AGC^GCT
ApaLI 707 GTGCAC G^TGCAC
AspEI 3142 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2039 TGCGCA TGC^GCA
BbvCI 6560 CCTCAGC CCTCAGC(-5/-2)
BciVI 989 GTATCC GTATCC(6/5)
BclI 3920 TGATCA T^GATCA
BfrI 3451 CTTAAG C^TTAAG
BlpI 6537 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 6537 GCTGAGC GC^T[ACGT]AGC
BsaHI 5738 GACGTC G[GA]^CG[CT]C
BsaWI 5780 ACCGGT [AT]^CCGG[AT]
BseRI 1606 GAGGAG GAGGAG(10/8)
BsmBI 1742 GAGACG CGTCTC(1/5)
Bsp1407I 1538 TGTACA T^GTACA
BsrBI 5909 CCGCTC CCGCTC(-3/-3)
BsrFI 5780 ACCGGT [GA]^CCGG[CT]
BsrGI 1538 TGTACA T^GTACA
BstUI 2788 CGCG CG^CG
CelII 6537 GCTGAGC GC^T[ACGT]AGC
Cfr10I 5780 ACCGGT [GA]^CCGG[CT]
Eam1105I 3142 GACTGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2021 AGCGCT AGC^GCT
EcoNI 5038 CCTCCAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3411 GAATTC G^AATTC
EcoRV 3252 GATATC GAT^ATC
FbaI 3920 TGATCA T^GATCA
FspI 2039 TGCGCA TGC^GCA
Hin1I 5738 GACGTC G[GA]^CG[CT]C
HincII 916 GTTGAC GT[CT]^[GA]AC
HindII 916 GTTGAC GT[CT]^[GA]AC
MfeI 1154 CAATTG C^AATTG
MunI 1154 CAATTG C^AATTG
MvnI 2788 CGCG CG^CG
NdeI 187 CATATG CA^TATG
PinAI 5780 ACCGGT A^CCGGT
Psp1406I 1990 AACGTT AA^CGTT
SacI 4909 GAGCTC GAGCT^C
ScaI 4019 AGTACT AGT^ACT
SexAI 5900 ACCAGGT A^CC[AT]GGT
SmaI 6419 CCCGGG CCC^GGG
SpeI 2184 ACTAGT A^CTAGT
SspBI 1538 TGTACA T^GTACA
XbaI 3474 TCTAGA T^CTAGA
XmaCI 6419 CCCGGG C^CCGGG
XmaI 6419 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AseI 1084 ATTAAT AT^TAAT
2653 ATTAAT
AsnI 1084 ATTAAT AT^TAAT
2653 ATTAAT
BanI 3874 GGTGCC G^G[CT][GA]CC
6553 GGTGCC
BglI 2538 GCCTGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2791 GCCGCACAGGC
BsaAI 2957 TACGTG [CT]AC^GT[GA]
5051 TACGTG
BsaI 5560 GAGACC GGTCTC(1/5)
2155 GGTCTC
BspDI 3166 ATCGAT AT^CGAT
5500 ATCGAT
BspHI 1518 TCATGA T^CATGA
2495 TCATGA
BspLU11I 1353 ACATGT A^CATGT
4676 ACATGT
BstEII 5025 GGTGACC G^GT[ACGT]ACC
6465 GGTAACC
BstPI 5025 GGTGACC G^GT[ACGT]ACC
6465 GGTAACC
ClaI 3166 ATCGAT AT^CGAT
5500 ATCGAT
DraII 4806 AGGACCT [GA]G^G[ACGT]CC[CT]
5980 AGGACCT
EcoO109I 4806 AGGACCT [GA]G^G[ACGT]CC[CT]
5980 AGGACCT
EcoO65I 5025 GGTGACC G^GT[ACGT]ACC
6465 GGTAACC
HaeII 2021 AGCGCT [GA]GCGC^[CT]
4094 AGCGCC
HgaI 1776 GCGTC GACGC(5/10)
4209 GCGTC
PaeR7I 2747 CTCGAG C^TCGAG
3406 CTCGAG
PflMI 715 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1871 CCATTGACTGG
PpuMI 4806 AGGACCT [GA]G^G[AT]CC[CT]
5980 AGGACCT
PshBI 1084 ATTAAT AT^TAAT
2653 ATTAAT
PstI 4262 CTGCAG CTGCA^G
6161 CTGCAG
RcaI 1518 TCATGA T^CATGA
2495 TCATGA
SapI 3445 GAAGAGC GCTCTTC(1/4)
4906 GAAGAGC
SphI 764 GCATGC GCATG^C
4330 GCATGC
Van91I 715 CCAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1871 CCATTGACTGG
XhoI 2747 CTCGAG C^TCGAG
3406 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BalI BamHI
BanII BbsI BbvI BcnI BfaI
BglII BmyI BpmI BpuAI BsaBI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
Eco81I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII XmnI