##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
Acc65I AccIII AflII AgeI AhdI
Alw44I AocI ApaLI AscI Asp700I
Asp718I AspEI AviII AvrII BbeI
BbrPI BfrI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsmBI BspDI BspEI BspHI BsrBI
BssHII Bst1107I BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoRI FseI
FspI HgaI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmaCI PmeI
PmlI PvuI RcaI RsrII SacII
SalI SapI SfiI SfoI SgrAI
SmaI SnaBI SwaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 2084 GACGTC GACGT^C
AccII 2637 CGCG CG^CG
AclI 959 AACGTT AA^CGTT
AcyI 2084 GACGTC G[GA]^CG[CT]C
AflIII 441 ACATGT A^C[GA][CT]GT
AlwNI 3426 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 879 AGCGCT AGC^GCT
ApaI 472 GGGCCC GGGCC^C
AvaI 37 CTCGGG C^[CT]CG[GA]G
BamHI 784 GGATCC G^GATCC
BbvCI 1966 GCTGAGG CCTCAGC(-5/-2)
BcgI 2848 CGAGCAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 57 TGATCA T^GATCA
BcnI 3906 CCCGG CC^[GC]GG
BglI 2375 GCCATTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 2084 GACGTC G[GA]^CG[CT]C
BsiWI 1196 CGTACG C^GTACG
BsmI 3773 GAATGC GAATGC(1/-1)
BsoBI 37 CTCGGG C^[CT]CG[GA]G
BspLU11I 441 ACATGT A^CATGT
BsrFI 567 ACCGGC [GA]^CCGG[CT]
BstAPI 2239 GCAGATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3909 GGTCACC G^GT[ACGT]ACC
BstPI 3909 GGTCACC G^GT[ACGT]ACC
BstUI 2637 CGCG CG^CG
BstXI 3872 CCATGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 567 ACCGGC [GA]^CCGG[CT]
Eco47III 879 AGCGCT AGC^GCT
EcoO65I 3909 GGTCACC G^GT[ACGT]ACC
FbaI 57 TGATCA T^GATCA
Hin1I 2084 GACGTC G[GA]^CG[CT]C
HindIII 4127 AAGCTT A^AGCTT
MvnI 2637 CGCG CG^CG
NciI 3906 CCCGG CC^[GC]GG
NcoI 3930 CCATGG C^CATGG
PshAI 2923 GACGGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 959 AACGTT AA^CGTT
SbfI 1063 CCTGCAGG CCTGCA^GG
SexAI 2709 ACCTGGT A^CC[AT]GGT
SpeI 74 ACTAGT A^CTAGT
SphI 1510 GCATGC GCATG^C
Sse8387I 1063 CCTGCAGG CCTGCA^GG
SspI 3204 AATATT AAT^ATT
StuI 2503 AGGCCT AGG^CCT
XbaI 991 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 70 GTCTAC GT^[AC][GT]AC
916 GTCTAC
AseI 2056 ATTAAT AT^TAAT
2534 ATTAAT
AsnI 2056 ATTAAT AT^TAAT
2534 ATTAAT
AspI 64 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
80 GACGATGTC
BanI 570 GGCACC G^G[CT][GA]CC
2540 GGTGCC
BbsI 89 GAAGAC GAAGAC(2/6)
1729 GAAGAC
BciVI 1058 GGATAC GTATCC(6/5)
816 GTATCC
BlpI 3765 GCTCAGC GC^T[ACGT]AGC
3824 GCTGAGC
Bpu1102I 3765 GCTCAGC GC^T[ACGT]AGC
3824 GCTGAGC
BpuAI 89 GAAGAC GAAGAC(2/6)
1729 GAAGAC
BsmAI 3393 GTCTC GTCTC(1/5)
3833 GTCTC
BstBI 1307 TTCGAA TT^CGAA
2498 TTCGAA
CelII 3765 GCTCAGC GC^T[ACGT]AGC
3824 GCTGAGC
DraIII 1043 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
4027 CACACAGTG
DsaI 3508 CCGTGG C^C[GA][CT]GG
3930 CCATGG
EcoNI 478 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
820 CCTCATTAAGG
EcoT22I 1757 ATGCAT ATGCA^T
2114 ATGCAT
HapII 568 CCGG C^CGG
3907 CCGG
HpaII 568 CCGG C^CGG
3907 CCGG
MaeII 960 ACGT A^CGT
2085 ACGT
MspI 568 CCGG C^CGG
3907 CCGG
NsiI 1757 ATGCAT ATGCA^T
2114 ATGCAT
NspV 1307 TTCGAA TT^CGAA
2498 TTCGAA
PflFI 64 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
80 GACGATGTC
PflMI 2278 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3503 CCAGTCCGTGG
PpuMI 1396 GGGTCCT [GA]G^G[AT]CC[CT]
2928 GGGTCCC
PshBI 2056 ATTAAT AT^TAAT
2534 ATTAAT
SfuI 1307 TTCGAA TT^CGAA
2498 TTCGAA
Tth111I 64 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
80 GACGATGTC
Van91I 2278 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3503 CCAGTCCGTGG
XcmI 435 CCACTGACATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1674 CCATGAAGGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BalI BanII
BbvI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I Bsp1407I BspMI BsrDI
BsrGI BsrI BssKI BssSI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII