##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AccII AccIII AgeI AhdI Aor51HI
ApaI AscI AspEI BamHI BbeI
BcgI BciVI BglI BlpI Bpu1102I
BsaI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspHI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstUI CelII Cfr10I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI HaeII HgaI
HpaI KasI KspI MluI MroI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PaeR7I
PinAI PmeI PpuMI PshAI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 1270 GACGTC GACGT^C
Acc65I 2815 GGTACC G^GTACC
AccI 3782 GTATAC GT^[AC][GT]AC
AclI 2688 AACGTT AA^CGTT
AcyI 1270 GACGTC G[GA]^CG[CT]C
AocI 1744 CCTCAGG CC^T[ACGT]AGG
AseI 996 ATTAAT AT^TAAT
AsnI 996 ATTAAT AT^TAAT
Asp700I 3591 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2815 GGTACC G^GTACC
AspI 524 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 180 CCCGAG C^[CT]CG[GA]G
AviII 2454 TGCGCA TGC^GCA
BbrPI 2430 CACGTG CAC^GTG
BcnI 1641 CCGGG CC^[GC]GG
BglII 18 AGATCT A^GATCT
BsaAI 2430 CACGTG [CT]AC^GT[GA]
BsaHI 1270 GACGTC G[GA]^CG[CT]C
BsgI 150 CTGCAC GTGCAG(16/14)
BsmBI 588 GAGACG CGTCTC(1/5)
BsoBI 180 CCCGAG C^[CT]CG[GA]G
Bst1107I 3782 GTATAC GTA^TAC
BstAPI 1413 GCACGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 363 CCATATAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3782 GTATAC GTA^TAC
Bsu36I 1744 CCTCAGG CC^T[ACGT]AGG
CfoI 2455 GCGC GCG^C
DraII 2959 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eco81I 1744 CCTCAGG CC^T[ACGT]AGG
EcoO109I 2959 GGGGCCT [GA]G^G[ACGT]CC[CT]
FspI 2454 TGCGCA TGC^GCA
HapII 1641 CCGG C^CGG
HhaI 2455 GCGC GCG^C
Hin1I 1270 GACGTC G[GA]^CG[CT]C
HinP1I 2455 GCGC G^CGC
HindIII 1496 AAGCTT A^AGCTT
HpaII 1641 CCGG C^CGG
KpnI 2815 GGTACC GGTAC^C
MfeI 1945 CAATTG C^AATTG
MspI 1641 CCGG C^CGG
MunI 1945 CAATTG C^AATTG
NciI 1641 CCGGG CC^[GC]GG
PacI 47 TTAATTAA TTAAT^TAA
PflFI 524 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1015 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2430 CACGTG CAC^GTG
PmlI 2430 CACGTG CAC^GTG
PshBI 996 ATTAAT AT^TAAT
Psp1406I 2688 AACGTT AA^CGTT
SacI 601 GAGCTC GAGCT^C
SspI 2176 AATATT AAT^ATT
StuI 486 AGGCCT AGG^CCT
Tth111I 524 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1015 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2217 TCTAGA T^CTAGA
XcmI 3061 CCATTAGACATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3591 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AflII 1377 CTTAAG C^TTAAG
2245 CTTAAG
AflIII 681 ACATGT A^C[GA][CT]GT
1592 ACATGT
Alw44I 2427 GTGCAC G^TGCAC
2433 GTGCAC
ApaLI 2427 GTGCAC G^TGCAC
2433 GTGCAC
AvaII 86 GGTCC G^G[AT]CC
3585 GGACC
AvrII 323 CCTAGG C^CTAGG
864 CCTAGG
BanII 409 GAGCCC G[GA]GC[CT]^C
601 GAGCTC
BbsI 127 GAAGAC GAAGAC(2/6)
2931 GTCTTC
BclI 1825 TGATCA T^GATCA
1958 TGATCA
BfrI 1377 CTTAAG C^TTAAG
2245 CTTAAG
BlnI 323 CCTAGG C^CTAGG
864 CCTAGG
BpuAI 127 GAAGAC GAAGAC(2/6)
2931 GTCTTC
BsaBI 1078 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2204 GATTTTTATC
BsmFI 390 GGGAC GGGAC(10/14)
1643 GGGAC
BsmI 1332 GAATGC GAATGC(1/-1)
3595 GCATTC
Bsp1407I 2077 TGTACA T^GTACA
2089 TGTACA
BspLU11I 681 ACATGT A^CATGT
1592 ACATGT
BspMI 839 ACCTGC ACCTGC(4/8)
144 GCAGGT
BsrDI 1422 GCAATG GCAATG(2/0)
3002 GCAATG
BsrGI 2077 TGTACA T^GTACA
2089 TGTACA
BstF5I 1247 CATCC GGATG(2/0)
1565 GGATG
BstYI 18 AGATCT [GA]^GATC[CT]
1083 AGATCC
DraI 1787 TTTAAA TTT^AAA
3292 TTTAAA
DsaI 382 CCATGG C^C[GA][CT]GG
634 CCATGG
FbaI 1825 TGATCA T^GATCA
1958 TGATCA
FokI 1247 CATCC GGATG(9/13)
1565 GGATG
MamI 1078 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2204 GATTTTTATC
MflI 18 AGATCT [GA]^GATC[CT]
1083 AGATCC
NcoI 382 CCATGG C^CATGG
634 CCATGG
NdeI 1602 CATATG CA^TATG
3387 CATATG
PstI 246 CTGCAG CTGCA^G
1810 CTGCAG
PvuII 1066 CAGCTG CAG^CTG
2528 CAGCTG
SpeI 933 ACTAGT A^CTAGT
3417 ACTAGT
SspBI 2077 TGTACA T^GTACA
2089 TGTACA
TaqI 319 TCGA T^CGA
3139 TCGA
TthHB8I 319 TCGA T^CGA
3139 TCGA
XhoII 18 AGATCT [GA]^GATC[CT]
1083 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI BalI BanI
BbvCI BbvI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoNI EcoRII EcoT14I Fnu4HI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI