##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AviII AvrII BamHI BanII
BbeI BbvCI BcgI BcnI BglI
BlnI BlpI Bpu1102I BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaII KasI KpnI KspI
MluI MroI MspI MvnI NaeI
NarI NciI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmeI PpuMI PshAI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI Sse8387I SwaI
Van91I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AciI 1034 GCGG CCGC(-3/-1)
AclI 1077 AACGTT AA^CGTT
AflII 3009 CTTAAG C^TTAAG
AflIII 2653 ACATGT A^C[GA][CT]GT
AocI 2205 CCTCAGG CC^T[ACGT]AGG
AspI 2727 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 103 CACGTG CAC^GTG
BfrI 3009 CTTAAG C^TTAAG
BsaBI 1145 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 1940 TGTACA T^GTACA
BspLU11I 2653 ACATGT A^CATGT
BspMI 2530 GCAGGT ACCTGC(4/8)
BsrGI 1940 TGTACA T^GTACA
Bsu36I 2205 CCTCAGG CC^T[ACGT]AGG
DraII 1231 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 100 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2205 CCTCAGG CC^T[ACGT]AGG
EcoNI 2323 CCTTGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1231 AGGCCCC [GA]G^G[ACGT]CC[CT]
HpaI 320 GTTAAC GTT^AAC
MamI 1145 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3203 CAATTG C^AATTG
MunI 3203 CAATTG C^AATTG
NheI 1361 GCTAGC G^CTAGC
PflFI 2727 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 103 CACGTG CAC^GTG
PmlI 103 CACGTG CAC^GTG
Psp1406I 1077 AACGTT AA^CGTT
PstI 791 CTGCAG CTGCA^G
SnaBI 696 TACGTA TAC^GTA
SpeI 3797 ACTAGT A^CTAGT
SphI 2901 GCATGC GCATG^C
SspBI 1940 TGTACA T^GTACA
SspI 3855 AATATT AAT^ATT
Tth111I 2727 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 626 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
799 CAGGAGCTG
AspHI 433 GTGCTC G[AT]GC[AT]^C
4053 GTGCTC
AvaI 774 CCCGAG C^[CT]CG[GA]G
1353 CCCGAG
BanI 400 GGTGCC G^G[CT][GA]CC
3587 GGTGCC
BciVI 1781 GGATAC GTATCC(6/5)
2977 GGATAC
BsgI 308 CTGCAC GTGCAG(16/14)
4143 CTGCAC
BsiHKAI 433 GTGCTC G[AT]GC[AT]^C
4053 GTGCTC
BsmI 384 GAATGC GAATGC(1/-1)
4304 GAATGC
BsoBI 774 CCCGAG C^[CT]CG[GA]G
1353 CCCGAG
BspDI 1734 ATCGAT AT^CGAT
3969 ATCGAT
ClaI 1734 ATCGAT AT^CGAT
3969 ATCGAT
DrdI 1523 GACTCTGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2946 GACAATCTTGTC
DsaI 1545 CCATGG C^C[GA][CT]GG
1858 CCATGG
EcoRV 3072 GATATC GAT^ATC
3086 GATATC
EcoT22I 1459 ATGCAT ATGCA^T
1984 ATGCAT
HindIII 195 AAGCTT A^AGCTT
2455 AAGCTT
NcoI 1545 CCATGG C^CATGG
1858 CCATGG
NsiI 1459 ATGCAT ATGCA^T
1984 ATGCAT
Tsp45I 1016 GTGAC ^GT[GC]AC
1064 GTGAC
XcmI 2067 CCAAGTGAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3229 CCATACTGGAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AseI AsnI AvaII BalI BbsI
BbvI BclI BfaI BglII BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XbaI XhoII