##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI AlwNI
Aor51HI AscI Asp700I Asp718I AspEI
AspI AviII BamHI BanI BbeI
BbrPI BbvCI BclI BglI BsaAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BspEI BspLU11I BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I KasI KpnI MaeII
MluI MroI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI SacI SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SwaI
Tth111I XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Alw44I 2345 GTGCAC G^TGCAC
AocI 3189 CCTGAGG CC^T[ACGT]AGG
ApaI 535 GGGCCC GGGCC^C
ApaLI 2345 GTGCAC G^TGCAC
AspHI 2345 GTGCAC G[AT]GC[AT]^C
AvaI 113 CCCGAG C^[CT]CG[GA]G
AvrII 2624 CCTAGG C^CTAGG
BcgI 2302 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2996 GGATAC GTATCC(6/5)
BcnI 69 CCCGG CC^[GC]GG
BlnI 2624 CCTAGG C^CTAGG
BlpI 816 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 816 GCTGAGC GC^T[ACGT]AGC
BsaI 43 GAGACC GGTCTC(1/5)
BsiHKAI 2345 GTGCAC G[AT]GC[AT]^C
BsoBI 113 CCCGAG C^[CT]CG[GA]G
BspDI 691 ATCGAT AT^CGAT
BsrBI 105 GAGCGG CCGCTC(-3/-3)
BsrFI 56 ACCGGC [GA]^CCGG[CT]
Bsu36I 3189 CCTGAGG CC^T[ACGT]AGG
CelII 816 GCTGAGC GC^T[ACGT]AGC
CfoI 2561 GCGC GCG^C
Cfr10I 56 ACCGGC [GA]^CCGG[CT]
ClaI 691 ATCGAT AT^CGAT
CpoI 53 CGGACCG CG^G[AT]CCG
DrdI 3752 GACAGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3189 CCTGAGG CC^T[ACGT]AGG
EcoRI 3979 GAATTC G^AATTC
HhaI 2561 GCGC GCG^C
HinP1I 2561 GCGC G^CGC
HincII 501 GTTAAC GT[CT]^[GA]AC
HindII 501 GTTAAC GT[CT]^[GA]AC
HpaI 501 GTTAAC GTT^AAC
MfeI 2147 CAATTG C^AATTG
MunI 2147 CAATTG C^AATTG
NciI 69 CCCGG CC^[GC]GG
PflMI 1396 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 715 CAGCTG CAG^CTG
RsrII 53 CGGACCG CG^G[AT]CCG
SexAI 383 ACCTGGT A^CC[AT]GGT
SpeI 2157 ACTAGT A^CTAGT
StuI 3356 AGGCCT AGG^CCT
Van91I 1396 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1005 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccII 31 CGCG CG^CG
84 CGCG
AflII 1132 CTTAAG C^TTAAG
2044 CTTAAG
AseI 2139 ATTAAT AT^TAAT
2865 ATTAAT
AsnI 2139 ATTAAT AT^TAAT
2865 ATTAAT
BalI 2360 TGGCCA TGG^CCA
3433 TGGCCA
BanII 116 GAGCCC G[GA]GC[CT]^C
535 GGGCCC
BfrI 1132 CTTAAG C^TTAAG
2044 CTTAAG
BglII 950 AGATCT A^GATCT
1639 AGATCT
BsaBI 850 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
945 GATAAAGATC
BseRI 349 CTCCTC GAGGAG(10/8)
2921 CTCCTC
BsmI 3962 GAATGC GAATGC(1/-1)
2665 GCATTC
Bsp1407I 2843 TGTACA T^GTACA
3899 TGTACA
BspHI 1922 TCATGA T^CATGA
3810 TCATGA
BspMI 1429 ACCTGC ACCTGC(4/8)
821 GCAGGT
BsrGI 2843 TGTACA T^GTACA
3899 TGTACA
BstUI 31 CGCG CG^CG
84 CGCG
BstXI 2323 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3089 CCATGACCTTGG
BstYI 950 AGATCT [GA]^GATC[CT]
1639 AGATCT
DsaI 30 CCGCGG C^C[GA][CT]GG
83 CCGCGG
EaeI 2360 TGGCCA [CT]^GGCC[GA]
3433 TGGCCA
EcoNI 120 CCTGTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
486 CCTGCAAAAGG
FauI 82 CCCGC CCCGC(4/6)
107 GCGGG
HapII 57 CCGG C^CGG
70 CCGG
HpaII 57 CCGG C^CGG
70 CCGG
HphI 2237 TCACC GGTGA(8/7)
3074 TCACC
KspI 30 CCGCGG CCGC^GG
83 CCGCGG
MamI 850 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
945 GATAAAGATC
MflI 950 AGATCT [GA]^GATC[CT]
1639 AGATCT
MluNI 2360 TGGCCA TGG^CCA
3433 TGGCCA
MscI 2360 TGGCCA TGG^CCA
3433 TGGCCA
MspI 57 CCGG C^CGG
70 CCGG
MvnI 31 CGCG CG^CG
84 CGCG
PpuMI 109 GGGACCC [GA]G^G[AT]CC[CT]
368 AGGTCCT
PshBI 2139 ATTAAT AT^TAAT
2865 ATTAAT
RcaI 1922 TCATGA T^CATGA
3810 TCATGA
SacII 30 CCGCGG CCGC^GG
83 CCGCGG
SapI 102 GAAGAGC GCTCTTC(1/4)
667 GAAGAGC
SspBI 2843 TGTACA T^GTACA
3899 TGTACA
TaqI 692 TCGA T^CGA
3750 TCGA
TthHB8I 692 TCGA T^CGA
3750 TCGA
XhoII 950 AGATCT [GA]^GATC[CT]
1639 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HindIII HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PshAI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI