##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AspEI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BbvCI BciVI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI BspEI BspHI
BsrBI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI Bsu36I CelII
CfoI CpoI DraII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1744 GGTACC G^GTACC
AccI 1381 GTATAC GT^[AC][GT]AC
AflII 1386 CTTAAG C^TTAAG
AgeI 2546 ACCGGT A^CCGGT
Asp718I 1744 GGTACC G^GTACC
AspHI 3169 GAGCAC G[AT]GC[AT]^C
AvaII 1848 GGACC G^G[AT]CC
BamHI 2455 GGATCC G^GATCC
BanI 1744 GGTACC G^G[CT][GA]CC
BanII 896 GGGCTC G[GA]GC[CT]^C
BbsI 1786 GAAGAC GAAGAC(2/6)
BcgI 3174 CGATGATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1386 CTTAAG C^TTAAG
BpuAI 1786 GAAGAC GAAGAC(2/6)
BsaWI 2546 ACCGGT [AT]^CCGG[AT]
BseRI 923 CTCCTC GAGGAG(10/8)
BsiHKAI 3169 GAGCAC G[AT]GC[AT]^C
Bsp1407I 3650 TGTACA T^GTACA
BspDI 2123 ATCGAT AT^CGAT
BspMI 2415 ACCTGC ACCTGC(4/8)
BsrFI 2546 ACCGGT [GA]^CCGG[CT]
BsrGI 3650 TGTACA T^GTACA
Bst1107I 1381 GTATAC GTA^TAC
BstXI 1063 CCAAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1381 GTATAC GTA^TAC
Cfr10I 2546 ACCGGT [GA]^CCGG[CT]
Cfr13I 1848 GGACC G^G[ACGT]CC
ClaI 2123 ATCGAT AT^CGAT
EcoNI 8 CCTGTAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 948 ATGCAT ATGCA^T
FauI 2783 CCCGC CCCGC(4/6)
HapII 2547 CCGG C^CGG
HincII 509 GTTGAC GT[CT]^[GA]AC
HindII 509 GTTGAC GT[CT]^[GA]AC
HpaII 2547 CCGG C^CGG
KpnI 1744 GGTACC GGTAC^C
MspI 2547 CCGG C^CGG
NheI 2842 GCTAGC G^CTAGC
NsiI 948 ATGCAT ATGCA^T
PflMI 327 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2546 ACCGGT A^CCGGT
Sau96I 1848 GGACC G^G[ACGT]CC
SspBI 3650 TGTACA T^GTACA
Van91I 327 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 513 ACATGT A^C[GA][CT]GT
1935 ACATGT
AlwNI 1093 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2263 CAGTTGCTG
BclI 861 TGATCA T^GATCA
1575 TGATCA
BspLU11I 513 ACATGT A^CATGT
1935 ACATGT
Cac8I 2824 GCAAGC GC[ACGT]^[ACGT]GC
2842 GCTAGC
DraIII 262 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3608 CACAAGGTG
EarI 3885 CTCTTC CTCTTC(1/4)
2281 GAAGAG
FbaI 861 TGATCA T^GATCA
1575 TGATCA
HindIII 1470 AAGCTT A^AGCTT
2691 AAGCTT
Ksp632I 3885 CTCTTC CTCTTC(1/4)
2281 GAAGAG
MaeII 2889 ACGT A^CGT
3100 ACGT
MslI 951 CATCACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3172 CACGATGATG
MspA1I 1571 CAGCTG C[AC]G^C[GT]G
3092 CAGCTG
NdeI 2680 CATATG CA^TATG
3530 CATATG
NlaIV 1744 GGTACC GG[ACGT]^[ACGT]CC
2455 GGATCC
PvuII 1571 CAGCTG CAG^CTG
3092 CAGCTG
SpeI 2071 ACTAGT A^CTAGT
2094 ACTAGT
StuI 2103 AGGCCT AGG^CCT
3066 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI Asp700I BbvI
BfaI BmyI BpmI BsaI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI DdeI DpnII DraI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NspI PleI
PshBI RsaI Sau3AI ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI