##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccII AccIII AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AviII BalI BamHI BbeI
BbrPI BbvCI BfrI BglI BlpI
Bpu1102I BsaWI BseAI BsiEI BsiWI
BspEI BsrBI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I CelII
CpoI DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I EcoO65I FseI
FspI HgaI KasI KpnI KspI
MluI MluNI MroI MscI MvnI
NarI NcoI NheI NotI NruI
PacI PinAI PmaCI PmeI PmlI
PvuI RsrII SacII SbfI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 5281 GACGTC GACGT^C
AclI 1060 AACGTT AA^CGTT
AcyI 5281 GACGTC G[GA]^CG[CT]C
AhdI 4540 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 104 CCTCAGG CC^T[ACGT]AGG
Asp700I 2408 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4540 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2308 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2557 CGAAACAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 564 CACGTA [CT]AC^GT[GA]
BsaHI 5281 GACGTC G[GA]^CG[CT]C
BsaI 2037 GAGACC GGTCTC(1/5)
BsmBI 1319 GAGACG CGTCTC(1/5)
Bsp1407I 80 TGTACA T^GTACA
BspDI 2936 ATCGAT AT^CGAT
BsrDI 903 GCAATG GCAATG(2/0)
BsrGI 80 TGTACA T^GTACA
BssSI 477 CTCGTG CACGAG(-5/-1)
BstXI 3423 CCACTCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 104 CCTCAGG CC^T[ACGT]AGG
ClaI 2936 ATCGAT AT^CGAT
EaeI 2680 TGGCCG [CT]^GGCC[GA]
Eam1105I 4540 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 104 CCTCAGG CC^T[ACGT]AGG
EcoNI 3900 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4190 GATATC GAT^ATC
FauI 19 GCGGG CCCGC(4/6)
HaeII 1528 AGCGCC [GA]GCGC^[CT]
Hin1I 5281 GACGTC G[GA]^CG[CT]C
HindIII 3374 AAGCTT A^AGCTT
PaeR7I 333 CTCGAG C^TCGAG
PflFI 2308 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2404 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 453 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2463 GACCCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1060 AACGTT AA^CGTT
SalI 3150 GTCGAC G^TCGAC
SapI 2444 GCTCTTC GCTCTTC(1/4)
SexAI 2040 ACCAGGT A^CC[AT]GGT
SpeI 3473 ACTAGT A^CTAGT
SspBI 80 TGTACA T^GTACA
StuI 2619 AGGCCT AGG^CCT
Tth111I 2308 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2404 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 333 CTCGAG C^TCGAG
XmnI 2408 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AciI 19 GCGG CCGC(-3/-1)
1542 GCGG
AseI 3035 ATTAAT AT^TAAT
6025 ATTAAT
AsnI 3035 ATTAAT AT^TAAT
6025 ATTAAT
AvaI 333 CTCGAG C^[CT]CG[GA]G
995 CTCGGG
AvaII 454 GGTCC G^G[AT]CC
1338 GGTCC
AvrII 115 CCTAGG C^CTAGG
4423 CCTAGG
BanI 4337 GGCACC G^G[CT][GA]CC
4726 GGTGCC
BclI 1363 TGATCA T^GATCA
1774 TGATCA
BcnI 1 CCCGG CC^[GC]GG
621 CCCGG
BlnI 115 CCTAGG C^CTAGG
4423 CCTAGG
BsaBI 5177 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5532 GATCTTAATC
BsgI 3043 CTGCAC GTGCAG(16/14)
2544 GTGCAG
BsoBI 333 CTCGAG C^[CT]CG[GA]G
995 CTCGGG
BsrFI 2632 GCCGGC [GA]^CCGG[CT]
3783 GCCGGC
BstBI 1497 TTCGAA TT^CGAA
4215 TTCGAA
CfoI 1529 GCGC GCG^C
2731 GCGC
Cfr10I 2632 GCCGGC [GA]^CCGG[CT]
3783 GCCGGC
DraII 453 AGGTCCT [GA]G^G[ACGT]CC[CT]
4426 AGGCCCT
EcoO109I 453 AGGTCCT [GA]G^G[ACGT]CC[CT]
4426 AGGCCCT
EcoT22I 1329 ATGCAT ATGCA^T
2565 ATGCAT
FbaI 1363 TGATCA T^GATCA
1774 TGATCA
HhaI 1529 GCGC GCG^C
2731 GCGC
HinP1I 1529 GCGC G^CGC
2731 GCGC
HpaI 299 GTTAAC GTT^AAC
4411 GTTAAC
MamI 5177 GATATATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5532 GATCTTAATC
MfeI 2213 CAATTG C^AATTG
5190 CAATTG
MunI 2213 CAATTG C^AATTG
5190 CAATTG
NaeI 2632 GCCGGC GCC^GGC
3783 GCCGGC
NciI 1 CCCGG CC^[GC]GG
621 CCCGG
NgoMIV 2632 GCCGGC G^CCGGC
3783 GCCGGC
NsiI 1329 ATGCAT ATGCA^T
2565 ATGCAT
NspV 1497 TTCGAA TT^CGAA
4215 TTCGAA
PshBI 3035 ATTAAT AT^TAAT
6025 ATTAAT
PvuII 2547 CAGCTG CAG^CTG
4875 CAGCTG
ScaI 3139 AGTACT AGT^ACT
3305 AGTACT
SfuI 1497 TTCGAA TT^CGAA
4215 TTCGAA
XcmI 2766 CCATTTCCAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3420 CCACCACTCAGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI BanII
BbsI BbvI BciVI BfaI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspHI BspLU11I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI PstI
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII