##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AviII AvrII
BbeI BciVI BlnI BsaHI BsgI
BsiWI BsmBI BspDI BspHI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI Hin1I HpaI KasI
KpnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SphI Sse8387I SspI SwaI
XcmI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AflII 3606 CTTAAG C^TTAAG
AhdI 3789 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 3505 GGGCCC GGGCC^C
Asp700I 2469 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3789 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1986 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 566 TGGCCA TGG^CCA
BamHI 127 GGATCC G^GATCC
BanI 3628 GGTGCC G^G[CT][GA]CC
BbrPI 100 CACGTG CAC^GTG
BbvCI 867 GCTGAGG CCTCAGC(-5/-2)
BfrI 3606 CTTAAG C^TTAAG
BglII 232 AGATCT A^GATCT
BlpI 220 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 220 GCTGAGC GC^T[ACGT]AGC
BsaBI 1523 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3591 CGACCG CG[GA][CT]^CG
Bsp1407I 644 TGTACA T^GTACA
BsrBI 223 GAGCGG CCGCTC(-3/-3)
BsrDI 2049 CATTGC GCAATG(2/0)
BsrFI 1424 GCCGGT [GA]^CCGG[CT]
BsrGI 644 TGTACA T^GTACA
BstAPI 2100 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3563 CCACACATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 220 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1424 GCCGGT [GA]^CCGG[CT]
DraI 1144 TTTAAA TTT^AAA
Eam1105I 3789 GACCCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3680 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3362 GAATTC G^AATTC
EcoT22I 166 ATGCAT ATGCA^T
FauI 3724 CCCGC CCCGC(4/6)
HaeII 1468 AGCGCC [GA]GCGC^[CT]
HincII 1460 GTCAAC GT[CT]^[GA]AC
HindII 1460 GTCAAC GT[CT]^[GA]AC
HindIII 1673 AAGCTT A^AGCTT
MamI 1523 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 640 CAATTG C^AATTG
MluI 1 ACGCGT A^CGCGT
MluNI 566 TGGCCA TGG^CCA
MscI 566 TGGCCA TGG^CCA
MunI 640 CAATTG C^AATTG
NsiI 166 ATGCAT ATGCA^T
PflFI 1986 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 100 CACGTG CAC^GTG
PmlI 100 CACGTG CAC^GTG
SacI 3391 GAGCTC GAGCT^C
ScaI 997 AGTACT AGT^ACT
SmaI 133 CCCGGG CCC^GGG
SpeI 1314 ACTAGT A^CTAGT
SspBI 644 TGTACA T^GTACA
Tth111I 1986 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 133 CCCGGG C^CCGGG
XmaI 133 CCCGGG C^CCGGG
XmnI 2469 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 194 TCCGGA T^CCGGA
834 TCCGGA
AlwNI 1286 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
2101 CAGCTGCTG
AocI 990 CCTGAGG CC^T[ACGT]AGG
3597 CCTGAGG
BbsI 2412 GAAGAC GAAGAC(2/6)
2955 GAAGAC
BcgI 1812 CGATGCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
509 GCAATACCTTCG
BclI 1229 TGATCA T^GATCA
3270 TGATCA
BpuAI 2412 GAAGAC GAAGAC(2/6)
2955 GAAGAC
BseAI 194 TCCGGA T^CCGGA
834 TCCGGA
BspEI 194 TCCGGA T^CCGGA
834 TCCGGA
BspLU11I 290 ACATGT A^CATGT
3567 ACATGT
BssSI 3476 CACGAG CACGAG(-5/-1)
2397 CTCGTG
Bsu36I 990 CCTGAGG CC^T[ACGT]AGG
3597 CCTGAGG
Eco81I 990 CCTGAGG CC^T[ACGT]AGG
3597 CCTGAGG
FbaI 1229 TGATCA T^GATCA
3270 TGATCA
HgaI 2415 GACGC GACGC(5/10)
3843 GCGTC
KspI 8 CCGCGG CCGC^GG
3725 CCGCGG
MroI 194 TCCGGA T^CCGGA
834 TCCGGA
PaeR7I 1019 CTCGAG C^TCGAG
1949 CTCGAG
SacII 8 CCGCGG CCGC^GG
3725 CCGCGG
StuI 1724 AGGCCT AGG^CCT
2266 AGGCCT
XbaI 936 TCTAGA T^CTAGA
3465 TCTAGA
XhoI 1019 CTCGAG C^TCGAG
1949 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaAI BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII