##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflIII AgeI AhdI AlwI
Aor51HI ApaI AscI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Cfr10I CpoI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoT22I FauI
FseI FspI HaeII HgaI Hin1I
KasI KpnI KspI MamI MflI
MluI MroI MspA1I MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SwaI Tth111I
XbaI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AclI 4275 AACGTT AA^CGTT
AflII 913 CTTAAG C^TTAAG
Alw44I 2818 GTGCAC G^TGCAC
AlwNI 629 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2818 GTGCAC G^TGCAC
AseI 3171 ATTAAT AT^TAAT
AsnI 3171 ATTAAT AT^TAAT
Asp700I 4120 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 3060 TGGCCA TGG^CCA
BanII 361 GAGCTC G[GA]GC[CT]^C
BbvCI 2138 CCTCAGC CCTCAGC(-5/-2)
BcgI 2869 CGATTCTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 735 GGATAC GTATCC(6/5)
BclI 417 TGATCA T^GATCA
BcnI 2116 CCGGG CC^[GC]GG
BfrI 913 CTTAAG C^TTAAG
BlpI 838 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 838 GCTGAGC GC^T[ACGT]AGC
BspDI 3274 ATCGAT AT^CGAT
BspMI 656 GCAGGT ACCTGC(4/8)
BssSI 2989 CTCGTG CACGAG(-5/-1)
CelII 838 GCTGAGC GC^T[ACGT]AGC
CfoI 384 GCGC GCG^C
ClaI 3274 ATCGAT AT^CGAT
DraIII 3320 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3039 CCATGG C^C[GA][CT]GG
EaeI 3060 TGGCCA [CT]^GGCC[GA]
EcoNI 207 CCTAGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 646 GATATC GAT^ATC
EcoT14I 3039 CCATGG C^C[AT][AT]GG
FbaI 417 TGATCA T^GATCA
HhaI 384 GCGC GCG^C
HinP1I 384 GCGC G^CGC
HpaI 3329 GTTAAC GTT^AAC
MaeII 4276 ACGT A^CGT
MfeI 3201 CAATTG C^AATTG
MluNI 3060 TGGCCA TGG^CCA
MscI 3060 TGGCCA TGG^CCA
MunI 3201 CAATTG C^AATTG
NciI 2116 CCGGG CC^[GC]GG
NcoI 3039 CCATGG C^CATGG
PshBI 3171 ATTAAT AT^TAAT
Psp1406I 4275 AACGTT AA^CGTT
SacI 361 GAGCTC GAGCT^C
ScaI 4174 AGTACT AGT^ACT
SpeI 4112 ACTAGT A^CTAGT
SphI 2168 GCATGC GCATG^C
StuI 2908 AGGCCT AGG^CCT
StyI 3039 CCATGG C^C[AT][AT]GG
XcmI 1957 CCAAGAGGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 4120 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 668 CCTCAGG CC^T[ACGT]AGG
2254 CCTCAGG
BbsI 1090 GAAGAC GAAGAC(2/6)
545 GTCTTC
BbvI 280 GCAGC GCAGC(8/12)
4031 GCTGC
BpuAI 1090 GAAGAC GAAGAC(2/6)
545 GTCTTC
BsmI 898 GAATGC GAATGC(1/-1)
1482 GCATTC
BsrDI 611 GCAATG GCAATG(2/0)
1518 GCAATG
BstAPI 2102 GCAACCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2844 GCACCTTCTGC
Bsu36I 668 CCTCAGG CC^T[ACGT]AGG
2254 CCTCAGG
Eco81I 668 CCTCAGG CC^T[ACGT]AGG
2254 CCTCAGG
EcoRI 1404 GAATTC G^AATTC
2755 GAATTC
Fnu4HI 280 GCAGC GC^[ACGT]GC
4031 GCTGC
HapII 2116 CCGG C^CGG
2281 CCGG
HindIII 195 AAGCTT A^AGCTT
406 AAGCTT
HpaII 2116 CCGG C^CGG
2281 CCGG
ItaI 280 GCAGC GC^[ACGT]GC
4031 GCTGC
MspI 2116 CCGG C^CGG
2281 CCGG
NlaIV 203 GGAACC GG[ACGT]^[ACGT]CC
822 GGTTCC
PflMI 1269 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3319 CCACCTTGTGG
PleI 3047 GAGTC GAGTC(4/5)
3729 GAGTC
PshAI 1935 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1964 GACTATGGTC
TaqI 928 TCGA T^CGA
3275 TCGA
TseI 280 GCAGC G^C[AT]GC
4031 GCTGC
TthHB8I 928 TCGA T^CGA
3275 TCGA
Van91I 1269 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3319 CCACCTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaII BfaI BmyI BpmI
BsaI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
FokI HaeIII HincII HindII HinfI
HphI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeII NlaIII NspI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TfiI Tru9I
Tsp45I Tsp509I TspRI