##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
ApaI AscI Asp718I AspI AviII
BbsI BbvCI BciVI BglI BglII
BpuAI BspDI BspLU11I BsrFI BssHII
Bst1107I BstZ17I Cfr10I ClaI EcoRV
FseI FspI KpnI MfeI MluI
MunI NaeI NcoI NgoMIV NheI
NotI NruI PaeR7I PflFI PinAI
PmeI Psp1406I PvuI SalI SbfI
ScaI SexAI SfiI SgrAI SnaBI
Sse8387I StuI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccIII 4836 TCCGGA T^CCGGA
AflII 1413 CTTAAG C^TTAAG
AflIII 2593 ACGTGT A^C[GA][CT]GT
AhdI 1314 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 570 GTGCAC G^TGCAC
Aor51HI 2167 AGCGCT AGC^GCT
ApaLI 570 GTGCAC G^TGCAC
Asp700I 3830 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1314 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3645 TGGCCA TGG^CCA
BamHI 1718 GGATCC G^GATCC
BbrPI 2778 CACGTG CAC^GTG
BcgI 3301 CGAGCTTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1413 CTTAAG C^TTAAG
BlpI 894 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 894 GCTAAGC GC^T[ACGT]AGC
BsaAI 2778 CACGTG [CT]AC^GT[GA]
BsaWI 4836 TCCGGA [AT]^CCGG[AT]
BseAI 4836 TCCGGA T^CCGGA
BsiEI 101 CGGCCG CG[GA][CT]^CG
BsiWI 2119 CGTACG C^GTACG
Bsp1407I 4640 TGTACA T^GTACA
BspEI 4836 TCCGGA T^CCGGA
BsrGI 4640 TGTACA T^GTACA
BssSI 2221 CACGAG CACGAG(-5/-1)
BstAPI 3150 GCATATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1339 TTCGAA TT^CGAA
CelII 894 GCTAAGC GC^T[ACGT]AGC
CpoI 2145 CGGTCCG CG^G[AT]CCG
DraII 1759 GGGACCT [GA]G^G[ACGT]CC[CT]
EagI 101 CGGCCG C^GGCCG
Eam1105I 1314 GACAAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 101 CGGCCG C^GGCCG
Eco47III 2167 AGCGCT AGC^GCT
Eco52I 101 CGGCCG C^GGCCG
EcoNI 259 CCTTGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1759 GGGACCT [GA]G^G[ACGT]CC[CT]
HpaI 4399 GTTAAC GTT^AAC
MluNI 3645 TGGCCA TGG^CCA
MroI 4836 TCCGGA T^CCGGA
MscI 3645 TGGCCA TGG^CCA
NdeI 1536 CATATG CA^TATG
NspI 2678 GCATGC [GA]CATG^[CT]
NspV 1339 TTCGAA TT^CGAA
PacI 852 TTAATTAA TTAAT^TAA
PmaCI 2778 CACGTG CAC^GTG
PmlI 2778 CACGTG CAC^GTG
PpuMI 1759 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 3962 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 2145 CGGTCCG CG^G[AT]CCG
SfuI 1339 TTCGAA TT^CGAA
SpeI 2385 ACTAGT A^CTAGT
SphI 2678 GCATGC GCATG^C
SspBI 4640 TGTACA T^GTACA
XmnI 3830 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AocI 1685 CCTAAGG CC^T[ACGT]AGG
4433 CCTCAGG
AvaI 115 CCCGGG C^[CT]CG[GA]G
311 CCCGGG
AvrII 264 CCTAGG C^CTAGG
636 CCTAGG
BbeI 127 GGCGCC GGCGC^C
2209 GGCGCC
BclI 2822 TGATCA T^GATCA
3268 TGATCA
BlnI 264 CCTAGG C^CTAGG
636 CCTAGG
BsaBI 653 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3393 GATAGACATC
BsmBI 2809 CGTCTC CGTCTC(1/5)
760 GAGACG
BsoBI 115 CCCGGG C^[CT]CG[GA]G
311 CCCGGG
BspHI 1210 TCATGA T^CATGA
4651 TCATGA
BsrBI 70 GAGCGG CCGCTC(-3/-3)
98 GAGCGG
BstXI 1048 CCAGTTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2235 CCACTGAGCTGG
Bsu36I 1685 CCTAAGG CC^T[ACGT]AGG
4433 CCTCAGG
Eco81I 1685 CCTAAGG CC^T[ACGT]AGG
4433 CCTCAGG
FbaI 2822 TGATCA T^GATCA
3268 TGATCA
HincII 996 GTCAAC GT[CT]^[GA]AC
4399 GTTAAC
HindII 996 GTCAAC GT[CT]^[GA]AC
4399 GTTAAC
HindIII 864 AAGCTT A^AGCTT
1127 AAGCTT
KasI 127 GGCGCC G^GCGCC
2209 GGCGCC
KspI 2149 CCGCGG CCGC^GG
2501 CCGCGG
MamI 653 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3393 GATAGACATC
NarI 127 GGCGCC GG^CGCC
2209 GGCGCC
PflMI 3648 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4167 CCAGCAAGTGG
PstI 2949 CTGCAG CTGCA^G
3029 CTGCAG
PvuII 1564 CAGCTG CAG^CTG
2139 CAGCTG
RcaI 1210 TCATGA T^CATGA
4651 TCATGA
SacII 2149 CCGCGG CCGC^GG
2501 CCGCGG
SfoI 127 GGCGCC GGC^GCC
2209 GGCGCC
SmaI 115 CCCGGG CCC^GGG
311 CCCGGG
Van91I 3648 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4167 CCAGCAAGTGG
XmaCI 115 CCCGGG C^CCGGG
311 CCCGGG
XmaI 115 CCCGGG C^CCGGG
311 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraIII DrdI
DsaI EaeI EarI EcoO65I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI PleI PshBI RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII