##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccIII AclI AhdI Alw44I AocI
Aor51HI ApaLI AscI AspEI AspI
AviII BbeI BbrPI BclI BseAI
BsiWI BspDI BspEI BspLU11I BsrBI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI Eam1105I Eco47III Eco81I EcoNI
EcoO65I FbaI FseI FspI HaeII
HgaI KasI KspI MfeI MluI
MroI MunI NarI NotI PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SbfI SfoI Sse8387I Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflIII 5922 ACACGT A^C[GA][CT]GT
AgeI 5795 ACCGGT A^CCGGT
AvrII 6231 CCTAGG C^CTAGG
BamHI 3137 GGATCC G^GATCC
BciVI 1117 GTATCC GTATCC(6/5)
BlnI 6231 CCTAGG C^CTAGG
BsaBI 1852 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 5885 CGGCCG CG[GA][CT]^CG
BsmBI 6136 GAGACG CGTCTC(1/5)
Bsp1407I 2303 TGTACA T^GTACA
BsrGI 2303 TGTACA T^GTACA
BstBI 1010 TTCGAA TT^CGAA
DsaI 266 CCATGG C^C[GA][CT]GG
EagI 5885 CGGCCG C^GGCCG
EclXI 5885 CGGCCG C^GGCCG
Eco52I 5885 CGGCCG C^GGCCG
FauI 4394 GCGGG CCCGC(4/6)
MamI 1852 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 3823 GCCGGC GCC^GGC
NcoI 266 CCATGG C^CATGG
NgoMIV 3823 GCCGGC G^CCGGC
NheI 1555 GCTAGC G^CTAGC
NruI 175 TCGCGA TCG^CGA
NspV 1010 TTCGAA TT^CGAA
PflMI 4346 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 5795 ACCGGT A^CCGGT
PpuMI 975 GGGACCT [GA]G^G[AT]CC[CT]
SalI 852 GTCGAC G^TCGAC
SexAI 1325 ACCAGGT A^CC[AT]GGT
SfuI 1010 TTCGAA TT^CGAA
SgrAI 5794 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1291 CCCGGG CCC^GGG
SnaBI 6496 TACGTA TAC^GTA
SpeI 2217 ACTAGT A^CTAGT
SphI 3092 GCATGC GCATG^C
SspBI 2303 TGTACA T^GTACA
Van91I 4346 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1291 CCCGGG C^CCGGG
XmaI 1291 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AatII 1466 GACGTC GACGT^C
6138 GACGTC
Acc65I 1322 GGTACC G^GTACC
5230 GGTACC
AccI 526 GTAGAC GT^[AC][GT]AC
852 GTCGAC
AcyI 1466 GACGTC G[GA]^CG[CT]C
6138 GACGTC
AflII 3154 CTTAAG C^TTAAG
5091 CTTAAG
AseI 4903 ATTAAT AT^TAAT
6787 ATTAAT
AsnI 4903 ATTAAT AT^TAAT
6787 ATTAAT
Asp718I 1322 GGTACC G^GTACC
5230 GGTACC
AvaI 240 CCCGAG C^[CT]CG[GA]G
1291 CCCGGG
BalI 1745 TGGCCA TGG^CCA
5855 TGGCCA
BcgI 3395 GCATTAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5626 GCAACTTCCTCG
BfrI 3154 CTTAAG C^TTAAG
5091 CTTAAG
BglII 2644 AGATCT A^GATCT
4475 AGATCT
BlpI 193 GCTCAGC GC^T[ACGT]AGC
3753 GCTGAGC
Bpu1102I 193 GCTCAGC GC^T[ACGT]AGC
3753 GCTGAGC
BsaHI 1466 GACGTC G[GA]^CG[CT]C
6138 GACGTC
BsaWI 20 ACCGGA [AT]^CCGG[AT]
5795 ACCGGT
BsoBI 240 CCCGAG C^[CT]CG[GA]G
1291 CCCGGG
BspHI 3002 TCATGA T^CATGA
5467 TCATGA
BsrFI 3823 GCCGGC [GA]^CCGG[CT]
5795 ACCGGT
BstAPI 3092 GCATGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6658 GCATTTATTGC
BstXI 3580 CCATTCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5395 CCACAGGGGTGG
CelII 193 GCTCAGC GC^T[ACGT]AGC
3753 GCTGAGC
Cfr10I 3823 GCCGGC [GA]^CCGG[CT]
5795 ACCGGT
Hin1I 1466 GACGTC G[GA]^CG[CT]C
6138 GACGTC
HindIII 3151 AAGCTT A^AGCTT
5081 AAGCTT
HpaI 6677 GTTAAC GTT^AAC
6692 GTTAAC
KpnI 1322 GGTACC GGTAC^C
5230 GGTACC
MluNI 1745 TGGCCA TGG^CCA
5855 TGGCCA
MscI 1745 TGGCCA TGG^CCA
5855 TGGCCA
NdeI 1775 CATATG CA^TATG
3849 CATATG
PshBI 4903 ATTAAT AT^TAAT
6787 ATTAAT
RcaI 3002 TCATGA T^CATGA
5467 TCATGA
SfiI 1341 GGCCTTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4846 GGCCAATGAGGCC
XcmI 1194 CCACCCCCGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
6333 CCACTTTGGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRI EcoRII
EcoRV EcoT14I EcoT22I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspI
StuI StyI SwaI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI