##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccII AgeI Aor51HI ApaI
AscI AviII AvrII BbeI BbvCI
BcnI BglI BlnI BlpI Bpu1102I
BsiEI BsiWI BspDI BsrBI BssHII
BssSI Bst1107I BstUI BstXI BstZ17I
CelII CfoI ClaI EagI EclXI
Eco47III Eco52I EcoNI FauI FseI
FspI HaeII HhaI HinP1I KasI
KspI MfeI MluI MunI MvnI
NaeI NarI NciI NgoMIV NotI
NruI PacI PaeR7I PinAI PmeI
PvuI SacII SalI SfiI SfoI
SgrAI SmaI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 5115 GGTACC G^GTACC
AclI 6930 AACGTT AA^CGTT
AcyI 5161 GACGCC G[GA]^CG[CT]C
Alw44I 313 GTGCAC G^TGCAC
ApaLI 313 GTGCAC G^TGCAC
Asp718I 5115 GGTACC G^GTACC
AspI 5920 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 308 CCCGAG C^[CT]CG[GA]G
BanII 5353 GAGCTC G[GA]GC[CT]^C
BbrPI 550 CACGTG CAC^GTG
BsaHI 5161 GACGCC G[GA]^CG[CT]C
BsoBI 308 CCCGAG C^[CT]CG[GA]G
Bsp1407I 7203 TGTACA T^GTACA
BspMI 2185 ACCTGC ACCTGC(4/8)
BsrDI 5782 GCAATG GCAATG(2/0)
BsrFI 997 ACCGGC [GA]^CCGG[CT]
BsrGI 7203 TGTACA T^GTACA
BstBI 5966 TTCGAA TT^CGAA
BstEII 5465 GGTAACC G^GT[ACGT]ACC
BstPI 5465 GGTAACC G^GT[ACGT]ACC
Cfr10I 997 ACCGGC [GA]^CCGG[CT]
CpoI 6977 CGGTCCG CG^G[AT]CCG
DrdI 7059 GACATGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 5465 GGTAACC G^GT[ACGT]ACC
Hin1I 5161 GACGCC G[GA]^CG[CT]C
HincII 1491 GTTAAC GT[CT]^[GA]AC
HindII 1491 GTTAAC GT[CT]^[GA]AC
HindIII 7134 AAGCTT A^AGCTT
HpaI 1491 GTTAAC GTT^AAC
KpnI 5115 GGTACC GGTAC^C
NdeI 3832 CATATG CA^TATG
NheI 6938 GCTAGC G^CTAGC
NspV 5966 TTCGAA TT^CGAA
PflFI 5920 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 550 CACGTG CAC^GTG
PmlI 550 CACGTG CAC^GTG
PshAI 2946 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 6930 AACGTT AA^CGTT
RsrII 6977 CGGTCCG CG^G[AT]CCG
SacI 5353 GAGCTC GAGCT^C
SapI 7140 GAAGAGC GCTCTTC(1/4)
SbfI 7186 CCTGCAGG CCTGCA^GG
SfuI 5966 TTCGAA TT^CGAA
SnaBI 6425 TACGTA TAC^GTA
Sse8387I 7186 CCTGCAGG CCTGCA^GG
SspBI 7203 TGTACA T^GTACA
StuI 2144 AGGCCT AGG^CCT
Tth111I 5920 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 449 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 3032 GTCTAC GT^[AC][GT]AC
4179 GTCTAC
AccIII 302 TCCGGA T^CCGGA
7078 TCCGGA
AhdI 3611 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5432 GACAGAGTGTC
AspEI 3611 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5432 GACAGAGTGTC
BamHI 610 GGATCC G^GATCC
3904 GGATCC
BcgI 310 CGAGTGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
649 GCAGTTGTATCG
BclI 6232 TGATCA T^GATCA
6636 TGATCA
BglII 2337 AGATCT A^GATCT
5418 AGATCT
BsaBI 29 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4823 GATTAAAATC
BsaWI 302 TCCGGA [AT]^CCGG[AT]
7078 TCCGGA
BseAI 302 TCCGGA T^CCGGA
7078 TCCGGA
BseRI 5846 GAGGAG GAGGAG(10/8)
6352 GAGGAG
BsmBI 7023 CGTCTC CGTCTC(1/5)
5159 GAGACG
BsmI 5089 GAATGC GAATGC(1/-1)
2834 GCATTC
BspEI 302 TCCGGA T^CCGGA
7078 TCCGGA
BspHI 3250 TCATGA T^CATGA
4744 TCATGA
BspLU11I 1613 ACATGT A^CATGT
7060 ACATGT
DraIII 7088 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
7383 CACTCAGTG
Eam1105I 3611 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5432 GACAGAGTGTC
EcoRV 2025 GATATC GAT^ATC
5721 GATATC
FbaI 6232 TGATCA T^GATCA
6636 TGATCA
MamI 29 GATAATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4823 GATTAAAATC
MroI 302 TCCGGA T^CCGGA
7078 TCCGGA
MslI 2835 CATTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3665 CATGGAAATG
NcoI 625 CCATGG C^CATGG
2581 CCATGG
PflMI 218 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2717 CCATATTATGG
RcaI 3250 TCATGA T^CATGA
4744 TCATGA
ScaI 3427 AGTACT AGT^ACT
4249 AGTACT
SexAI 1320 ACCTGGT A^CC[AT]GGT
5595 ACCAGGT
SpeI 1642 ACTAGT A^CTAGT
6916 ACTAGT
Van91I 218 CCACTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2717 CCATATTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspHI AvaII
BalI BanI BbsI BbvI BciVI
BfaI BfrI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PshBI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI