##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI AspEI BcgI BciVI BclI
BfrI BglI BsaBI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HpaI MamI MluI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SalI SfiI SfuI SgrAI SmaI
SnaBI SpeI SspBI SspI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 1925 GGTACC G^GTACC
AccI 2721 GTCTAC GT^[AC][GT]AC
AcsI 2388 GAATTT [GA]^AATT[CT]
Aor51HI 658 AGCGCT AGC^GCT
ApoI 2388 GAATTT [GA]^AATT[CT]
Asp700I 152 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1925 GGTACC G^GTACC
AspI 1334 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1272 TGCGCA TGC^GCA
BbrPI 760 CACGTG CAC^GTG
BglII 2596 AGATCT A^GATCT
BsaAI 760 CACGTG [CT]AC^GT[GA]
BsaI 293 GAGACC GGTCTC(1/5)
BsmI 2034 GCATTC GAATGC(1/-1)
BsrDI 2211 CATTGC GCAATG(2/0)
BsrFI 1482 GCCGGT [GA]^CCGG[CT]
BstAPI 766 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1482 GCCGGT [GA]^CCGG[CT]
DraI 2866 TTTAAA TTT^AAA
Eco47III 658 AGCGCT AGC^GCT
FspI 1272 TGCGCA TGC^GCA
HincII 2137 GTCAAC GT[CT]^[GA]AC
HindII 2137 GTCAAC GT[CT]^[GA]AC
HindIII 2466 AAGCTT A^AGCTT
KpnI 1925 GGTACC GGTAC^C
KspI 1084 CCGCGG CCGC^GG
MfeI 2400 CAATTG C^AATTG
MseI 2867 TTAA T^TAA
MslI 2315 CACAGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2400 CAATTG C^AATTG
NcoI 2774 CCATGG C^CATGG
PaeR7I 699 CTCGAG C^TCGAG
PflFI 1334 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 760 CACGTG CAC^GTG
PmlI 760 CACGTG CAC^GTG
SacII 1084 CCGCGG CCGC^GG
ScaI 1844 AGTACT AGT^ACT
SexAI 483 ACCAGGT A^CC[AT]GGT
SphI 684 GCATGC GCATG^C
StuI 1324 AGGCCT AGG^CCT
SwaI 2865 ATTTAAAT ATTT^AAAT
Tru9I 2867 TTAA T^TAA
Tth111I 1334 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 699 CTCGAG C^TCGAG
XmnI 152 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 903 TCCGGA T^CCGGA
2008 TCCGGA
AlwNI 959 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
2090 CAGGGCCTG
AocI 1781 CCTCAGG CC^T[ACGT]AGG
2050 CCTGAGG
AvrII 341 CCTAGG C^CTAGG
1930 CCTAGG
BalI 165 TGGCCA TGG^CCA
2771 TGGCCA
BbeI 1080 GGCGCC GGCGC^C
1380 GGCGCC
BbvCI 1629 CCTCAGC CCTCAGC(-5/-2)
869 GCTGAGG
BlnI 341 CCTAGG C^CTAGG
1930 CCTAGG
BsaWI 903 TCCGGA [AT]^CCGG[AT]
2008 TCCGGA
BseAI 903 TCCGGA T^CCGGA
2008 TCCGGA
BsgI 2312 CTGCAC GTGCAG(16/14)
2711 CTGCAC
BspEI 903 TCCGGA T^CCGGA
2008 TCCGGA
BspHI 2044 TCATGA T^CATGA
2262 TCATGA
Bsu36I 1781 CCTCAGG CC^T[ACGT]AGG
2050 CCTGAGG
Eco81I 1781 CCTCAGG CC^T[ACGT]AGG
2050 CCTGAGG
KasI 1080 GGCGCC G^GCGCC
1380 GGCGCC
MaeII 761 ACGT A^CGT
1338 ACGT
MluNI 165 TGGCCA TGG^CCA
2771 TGGCCA
MroI 903 TCCGGA T^CCGGA
2008 TCCGGA
MscI 165 TGGCCA TGG^CCA
2771 TGGCCA
NarI 1080 GGCGCC GG^CGCC
1380 GGCGCC
RcaI 2044 TCATGA T^CATGA
2262 TCATGA
SacI 266 GAGCTC GAGCT^C
938 GAGCTC
SbfI 2095 CCTGCAGG CCTGCA^GG
2369 CCTGCAGG
SfoI 1080 GGCGCC GGC^GCC
1380 GGCGCC
SmlI 699 CTCGAG C^T[CT][GA]AG
941 CTCAAG
Sse8387I 2095 CCTGCAGG CCTGCA^GG
2369 CCTGCAGG
XbaI 171 TCTAGA T^CTAGA
1796 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BamHI BanI BanII BbsI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII