##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI AscI Asp718I
AspEI AviII BamHI BbeI BcgI
BglI BsaHI BseAI BsiWI BsmBI
BspDI BspEI BsrBI BsrFI BssHII
BstBI BstEII BstPI Cfr10I ClaI
CpoI Eam1105I Eco47III EcoNI EcoO65I
EcoRV FseI FspI HgaI Hin1I
KasI KpnI KspI MluI MroI
NaeI NarI NgoMIV NotI NruI
NspV PacI PinAI PmeI Psp1406I
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AvaI 1855 CTCGAG C^[CT]CG[GA]G
AvrII 7559 CCTAGG C^CTAGG
BbrPI 8000 CACGTG CAC^GTG
BbvCI 2445 GCTGAGG CCTCAGC(-5/-2)
BciVI 6142 GTATCC GTATCC(6/5)
BcnI 1563 CCGGG CC^[GC]GG
BlnI 7559 CCTAGG C^CTAGG
BsaAI 8000 CACGTG [CT]AC^GT[GA]
BsaBI 4599 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 5928 TCCGGT [AT]^CCGG[AT]
BsoBI 1855 CTCGAG C^[CT]CG[GA]G
Bsp1407I 7924 TGTACA T^GTACA
BsrGI 7924 TGTACA T^GTACA
BssSI 1369 CTCGTG CACGAG(-5/-1)
BstXI 1354 CCAATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 5865 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1516 GACTCCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 7541 CGGCCG C^GGCCG
EclXI 7541 CGGCCG C^GGCCG
Eco52I 7541 CGGCCG C^GGCCG
EcoT22I 5258 ATGCAT ATGCA^T
HaeII 11 GGCGCT [GA]GCGC^[CT]
MamI 4599 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 1563 CCGGG CC^[GC]GG
NheI 3789 GCTAGC G^CTAGC
NsiI 5258 ATGCAT ATGCA^T
PaeR7I 1855 CTCGAG C^TCGAG
PmaCI 8000 CACGTG CAC^GTG
PmlI 8000 CACGTG CAC^GTG
PpuMI 2089 AGGACCT [GA]G^G[AT]CC[CT]
PvuI 4460 CGATCG CGAT^CG
SacI 7282 GAGCTC GAGCT^C
SapI 2712 GAAGAGC GCTCTTC(1/4)
SphI 104 GCATGC GCATG^C
SspBI 7924 TGTACA T^GTACA
XhoI 1855 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 50 CGCG CG^CG
5472 CGCG
Alw44I 3496 GTGCAC G^TGCAC
4888 GTGCAC
ApaI 18 GGGCCC GGGCC^C
3952 GGGCCC
ApaLI 3496 GTGCAC G^TGCAC
4888 GTGCAC
BanI 68 GGCACC G^G[CT][GA]CC
3740 GGCACC
BclI 1424 TGATCA T^GATCA
1970 TGATCA
BsaI 1411 GAGACC GGTCTC(1/5)
3435 GGTCTC
BsiEI 4460 CGATCG CG[GA][CT]^CG
7541 CGGCCG
BsmFI 6080 GGGAC GGGAC(10/14)
1891 GTCCC
BspHI 413 TCATGA T^CATGA
4894 TCATGA
BspLU11I 4291 ACATGT A^CATGT
4993 ACATGT
Bst1107I 1191 GTATAC GTA^TAC
1474 GTATAC
BstAPI 761 GCAAAATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5590 GCAAAATTTGC
BstUI 50 CGCG CG^CG
5472 CGCG
BstZ17I 1191 GTATAC GTA^TAC
1474 GTATAC
EcoRI 540 GAATTC G^AATTC
2772 GAATTC
FbaI 1424 TGATCA T^GATCA
1970 TGATCA
HapII 1563 CCGG C^CGG
5929 CCGG
HpaII 1563 CCGG C^CGG
5929 CCGG
MspI 1563 CCGG C^CGG
5929 CCGG
MvnI 50 CGCG CG^CG
5472 CGCG
PflMI 6392 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7661 CCAGCAATTGG
PshAI 579 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3518 GACTTGAGTC
RcaI 413 TCATGA T^CATGA
4894 TCATGA
ScaI 2935 AGTACT AGT^ACT
4845 AGTACT
StuI 3079 AGGCCT AGG^CCT
7724 AGGCCT
Van91I 6392 CCATTATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
7661 CCAGCAATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI AocI
ApoI AseI AsnI Asp700I AspHI
AspI AvaII BalI BanII BbsI
BbvI BfaI BfrI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HindIII
HinfI HpaI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MluNI MnlI MscI
MseI MslI MspA1I MunI MvaI
MwoI NcoI NdeI NdeII NlaIII
NlaIV NspI PflFI PleI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SpeI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII XmnI