##################################
# NO CUT ENZYMES ( 44 enzymes) #
##################################
AatII AhdI Aor51HI AscI AspEI
AviII AvrII BbrPI BlnI BsiWI
BspDI BspLU11I BssHII BstBI BstEII
BstPI ClaI CpoI Eam1105I Eco47III
EcoO65I EcoT22I FspI MfeI MluI
MunI NruI NsiI NspV PacI
PaeR7I PmaCI PmeI PmlI PvuI
RsrII SalI ScaI SfuI SgrAI
SnaBI SpeI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 3557 TCCGGA T^CCGGA
AclI 5978 AACGTT AA^CGTT
AflIII 5008 ACGTGT A^C[GA][CT]GT
AgeI 4528 ACCGGT A^CCGGT
Alw44I 6696 GTGCAC G^TGCAC
ApaLI 6696 GTGCAC G^TGCAC
BamHI 7532 GGATCC G^GATCC
BciVI 1221 GGATAC GTATCC(6/5)
BglII 2795 AGATCT A^GATCT
BlpI 1528 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1528 GCTAAGC GC^T[ACGT]AGC
BsaBI 2335 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3557 TCCGGA T^CCGGA
BsmBI 5694 GAGACG CGTCTC(1/5)
BsmI 7294 GCATTC GAATGC(1/-1)
BspEI 3557 TCCGGA T^CCGGA
BspHI 4169 TCATGA T^CATGA
BsrDI 4741 GCAATG GCAATG(2/0)
BssSI 6906 CTCGTG CACGAG(-5/-1)
Bst1107I 1414 GTATAC GTA^TAC
BstZ17I 1414 GTATAC GTA^TAC
CelII 1528 GCTAAGC GC^T[ACGT]AGC
EcoRV 2962 GATATC GAT^ATC
FseI 6552 GGCCGGCC GGCCGG^CC
HindIII 370 AAGCTT A^AGCTT
HpaI 2107 GTTAAC GTT^AAC
KspI 3637 CCGCGG CCGC^GG
MamI 2335 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3557 TCCGGA T^CCGGA
NdeI 2854 CATATG CA^TATG
NotI 7685 GCGGCCGC GC^GGCCGC
PinAI 4528 ACCGGT A^CCGGT
PpuMI 5375 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 5314 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5978 AACGTT AA^CGTT
RcaI 4169 TCATGA T^CATGA
SacII 3637 CCGCGG CCGC^GG
SapI 3963 GAAGAGC GCTCTTC(1/4)
SbfI 2943 CCTGCAGG CCTGCA^GG
Sse8387I 2943 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 925 GTCTAC GT^[AC][GT]AC
1414 GTATAC
AseI 2097 ATTAAT AT^TAAT
2236 ATTAAT
AsnI 2097 ATTAAT AT^TAAT
2236 ATTAAT
AspI 919 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
7614 GACATCGTC
BcgI 2862 CGATAATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
116 GCAACCGCTTCG
BclI 303 TGATCA T^GATCA
3897 TGATCA
EagI 1738 CGGCCG C^GGCCG
7686 CGGCCG
EclXI 1738 CGGCCG C^GGCCG
7686 CGGCCG
Eco52I 1738 CGGCCG C^GGCCG
7686 CGGCCG
FbaI 303 TGATCA T^GATCA
3897 TGATCA
HincII 2107 GTTAAC GT[CT]^[GA]AC
2162 GTCAAC
HindII 2107 GTTAAC GT[CT]^[GA]AC
2162 GTCAAC
NheI 2173 GCTAGC G^CTAGC
2314 GCTAGC
PflFI 919 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
7614 GACATCGTC
PshBI 2097 ATTAAT AT^TAAT
2236 ATTAAT
SacI 1850 GAGCTC GAGCT^C
5720 GAGCTC
SexAI 2297 ACCTGGT A^CC[AT]GGT
6067 ACCTGGT
SfiI 5912 GGCCCTGTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
7462 GGCCAGGAGGGCC
SmaI 2912 CCCGGG CCC^GGG
5648 CCCGGG
SphI 4062 GCATGC GCATG^C
6722 GCATGC
SspI 1493 AATATT AAT^ATT
3649 AATATT
Tth111I 919 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
7614 GACATCGTC
XbaI 1062 TCTAGA T^CTAGA
3426 TCTAGA
XmaCI 2912 CCCGGG C^CCGGG
5648 CCCGGG
XmaI 2912 CCCGGG C^CCGGG
5648 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 149 enzymes) #
#########################################
Acc65I AccII AciI AcsI AcyI
AfaI AflII AluI AlwI AlwNI
AocI ApaI ApoI Asp700I Asp718I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BfrI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrFI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DraIII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
KpnI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmlI SspBI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmnI