##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII AccII AccIII AgeI Alw44I
Aor51HI ApaLI AscI AspI AviII
BcgI BsaWI BseAI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BssHII
BssSI BstAPI BstBI BstUI ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoRI FseI FspI HgaI HpaI
KspI MfeI MluI MroI MunI
MvnI NaeI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmeI
PshAI PvuI RsrII SacII SalI
SbfI SfiI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 2913 AACGTT AA^CGTT
AcyI 253 GGCGCC G[GA]^CG[CT]C
AhdI 4889 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 465 CCTTAGG CC^T[ACGT]AGG
ApaI 7675 GGGCCC GGGCC^C
AspEI 4889 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2059 GAGCTC G[AT]GC[AT]^C
BamHI 936 GGATCC G^GATCC
BbeI 253 GGCGCC GGCGC^C
BglI 80 GCCGAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1213 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1213 GCTGAGC GC^T[ACGT]AGC
BsaHI 253 GGCGCC G[GA]^CG[CT]C
BsaI 765 GAGACC GGTCTC(1/5)
BsiHKAI 2059 GAGCTC G[AT]GC[AT]^C
BsrFI 34 GCCGGT [GA]^CCGG[CT]
BstEII 945 GGTCACC G^GT[ACGT]ACC
BstPI 945 GGTCACC G^GT[ACGT]ACC
Bsu36I 465 CCTTAGG CC^T[ACGT]AGG
CelII 1213 GCTGAGC GC^T[ACGT]AGC
Cfr10I 34 GCCGGT [GA]^CCGG[CT]
DrdI 3272 GACATTAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4889 GACTTTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 465 CCTTAGG CC^T[ACGT]AGG
EcoO65I 945 GGTCACC G^GT[ACGT]ACC
EcoRV 6995 GATATC GAT^ATC
Hin1I 253 GGCGCC G[GA]^CG[CT]C
KasI 253 GGCGCC G^GCGCC
NarI 253 GGCGCC GG^CGCC
NdeI 7371 CATATG CA^TATG
NheI 7884 GCTAGC G^CTAGC
PaeR7I 7545 CTCGAG C^TCGAG
PpuMI 4895 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 2913 AACGTT AA^CGTT
SacI 2059 GAGCTC GAGCT^C
SexAI 346 ACCAGGT A^CC[AT]GGT
SfoI 253 GGCGCC GGC^GCC
StuI 726 AGGCCT AGG^CCT
XhoI 7545 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 4364 GGTACC G^GTACC
5941 GGTACC
AlwNI 4745 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
6476 CAGTTACTG
Asp700I 447 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4157 GAATACCTTC
Asp718I 4364 GGTACC G^GTACC
5941 GGTACC
AvaI 1 CTCGGG C^[CT]CG[GA]G
7545 CTCGAG
AvrII 1114 CCTAGG C^CTAGG
4738 CCTAGG
BalI 7051 TGGCCA TGG^CCA
7686 TGGCCA
BbrPI 359 CACGTG CAC^GTG
5301 CACGTG
BbvCI 339 CCTCAGC CCTCAGC(-5/-2)
6490 GCTGAGG
BciVI 178 GGATAC GTATCC(6/5)
3502 GGATAC
BclI 481 TGATCA T^GATCA
6784 TGATCA
BlnI 1114 CCTAGG C^CTAGG
4738 CCTAGG
BsoBI 1 CTCGGG C^[CT]CG[GA]G
7545 CTCGAG
BspHI 2169 TCATGA T^CATGA
2474 TCATGA
Bst1107I 1150 GTATAC GTA^TAC
3030 GTATAC
BstZ17I 1150 GTATAC GTA^TAC
3030 GTATAC
CfoI 89 GCGC GCG^C
254 GCGC
DraIII 1670 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
5077 CACCCTGTG
DsaI 656 CCATGG C^C[GA][CT]GG
7591 CCATGG
EaeI 7051 TGGCCA [CT]^GGCC[GA]
7686 TGGCCA
EcoNI 1726 CCTAGTATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3827 CCTTAGCTAGG
FbaI 481 TGATCA T^GATCA
6784 TGATCA
HaeII 88 GGCGCT [GA]GCGC^[CT]
253 GGCGCC
HhaI 89 GCGC GCG^C
254 GCGC
HinP1I 89 GCGC G^CGC
254 GCGC
HincII 3138 GTTGAC GT[CT]^[GA]AC
3461 GTCAAC
HindII 3138 GTTGAC GT[CT]^[GA]AC
3461 GTCAAC
KpnI 4364 GGTACC GGTAC^C
5941 GGTACC
MluNI 7051 TGGCCA TGG^CCA
7686 TGGCCA
MscI 7051 TGGCCA TGG^CCA
7686 TGGCCA
NcoI 656 CCATGG C^CATGG
7591 CCATGG
PmaCI 359 CACGTG CAC^GTG
5301 CACGTG
PmlI 359 CACGTG CAC^GTG
5301 CACGTG
RcaI 2169 TCATGA T^CATGA
2474 TCATGA
SapI 5467 GCTCTTC GCTCTTC(1/4)
5977 GCTCTTC
SpeI 4827 ACTAGT A^CTAGT
5143 ACTAGT
SphI 5060 GCATGC GCATG^C
5521 GCATGC
TaqI 6386 TCGA T^CGA
7546 TCGA
TthHB8I 6386 TCGA T^CGA
7546 TCGA
XcmI 1061 CCAACTTTGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1180 CCACATCCATTATGG
XmnI 447 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4157 GAATACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AflIII AluI AlwI ApoI AseI
AsnI AvaII BanI BanII BbsI
BbvI BcnI BfaI BfrI BglII
BmyI BpmI BpuAI BsaAI BsaBI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspLU11I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XbaI XhoII