##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AgeI AhdI AscI AseI
AsnI AspEI BsiWI Bsp1407I BspDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI Eam1105I
EcoO65I EcoRI EcoRV FseI HpaI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshBI PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SpeI Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 5798 GGTACC G^GTACC
AccI 5683 GTAGAC GT^[AC][GT]AC
AccIII 277 TCCGGA T^CCGGA
AclI 5528 AACGTT AA^CGTT
Alw44I 4259 GTGCAC G^TGCAC
ApaLI 4259 GTGCAC G^TGCAC
Asp718I 5798 GGTACC G^GTACC
AviII 2667 TGCGCA TGC^GCA
BamHI 3666 GGATCC G^GATCC
BbrPI 5427 CACGTG CAC^GTG
BcgI 5765 GCAGAGGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4536 GTATCC GTATCC(6/5)
BclI 6165 TGATCA T^GATCA
BsaBI 545 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 277 TCCGGA [AT]^CCGG[AT]
BseAI 277 TCCGGA T^CCGGA
BspEI 277 TCCGGA T^CCGGA
BspLU11I 6309 ACATGT A^CATGT
BsrDI 897 GCAATG GCAATG(2/0)
BssHII 4676 GCGCGC G^CGCGC
BstAPI 2824 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 848 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2411 GACAGCCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3624 CGGCCG C^GGCCG
EclXI 3624 CGGCCG C^GGCCG
Eco52I 3624 CGGCCG C^GGCCG
EcoT22I 6146 ATGCAT ATGCA^T
FbaI 6165 TGATCA T^GATCA
FspI 2667 TGCGCA TGC^GCA
HincII 5433 GTTGAC GT[CT]^[GA]AC
HindII 5433 GTTGAC GT[CT]^[GA]AC
KpnI 5798 GGTACC GGTAC^C
KspI 2834 CCGCGG CCGC^GG
MamI 545 GATATTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 277 TCCGGA T^CCGGA
NsiI 6146 ATGCAT ATGCA^T
PmaCI 5427 CACGTG CAC^GTG
PmlI 5427 CACGTG CAC^GTG
PshAI 3344 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5528 AACGTT AA^CGTT
SacII 2834 CCGCGG CCGC^GG
SnaBI 4118 TACGTA TAC^GTA
SphI 2664 GCATGC GCATG^C
XbaI 4785 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflII 506 CTTAAG C^TTAAG
599 CTTAAG
AflIII 654 ACGTGT A^C[GA][CT]GT
6309 ACATGT
Aor51HI 1413 AGCGCT AGC^GCT
2173 AGCGCT
BbeI 2702 GGCGCC GGCGC^C
2813 GGCGCC
BfrI 506 CTTAAG C^TTAAG
599 CTTAAG
BglI 3305 GCCAGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4703 GCCTTGAGGGC
BsaAI 4118 TACGTA [CT]AC^GT[GA]
5427 CACGTG
BsiEI 1354 CGACCG CG[GA][CT]^CG
3624 CGGCCG
BspHI 33 TCATGA T^CATGA
873 TCATGA
BspMI 2966 ACCTGC ACCTGC(4/8)
4483 ACCTGC
BsrBI 1573 CCGCTC CCGCTC(-3/-3)
3195 CCGCTC
Eco47III 1413 AGCGCT AGC^GCT
2173 AGCGCT
HindIII 256 AAGCTT A^AGCTT
6194 AAGCTT
KasI 2702 GGCGCC G^GCGCC
2813 GGCGCC
NaeI 893 GCCGGC GCC^GGC
2705 GCCGGC
NarI 2702 GGCGCC GG^CGCC
2813 GGCGCC
NgoMIV 893 GCCGGC G^CCGGC
2705 GCCGGC
PaeR7I 450 CTCGAG C^TCGAG
4341 CTCGAG
RcaI 33 TCATGA T^CATGA
873 TCATGA
SfoI 2702 GGCGCC GGC^GCC
2813 GGCGCC
SspI 11 AATATT AAT^ATT
841 AATATT
XhoI 450 CTCGAG C^TCGAG
4341 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp700I AspHI AspI AvaI
AvaII AvrII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XcmI XhoII XmaCI
XmaI XmnI