##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AclI AgeI ApaI
AscI Asp718I AviII AvrII BbeI
BciVI BclI BlnI BsaBI BsiEI
BsiWI BspDI BspMI BssHII Bst1107I
BstEII BstPI BstZ17I ClaI DrdI
EagI EclXI Eco52I EcoNI EcoO65I
EcoT22I FbaI FseI FspI KasI
KpnI KspI MamI MluI NarI
NheI NotI NruI NsiI PacI
PinAI PmeI Psp1406I PvuI SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SwaI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 438 TCCGGA T^CCGGA
AcyI 181 GGCGTC G[GA]^CG[CT]C
AflIII 2170 ACATGT A^C[GA][CT]GT
Alw44I 1701 GTGCAC G^TGCAC
AocI 2684 CCTTAGG CC^T[ACGT]AGG
Aor51HI 609 AGCGCT AGC^GCT
ApaLI 1701 GTGCAC G^TGCAC
BbrPI 977 CACGTG CAC^GTG
BbsI 674 GAAGAC GAAGAC(2/6)
BcgI 5294 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 420 AGATCT A^GATCT
BlpI 88 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 88 GCTCAGC GC^T[ACGT]AGC
BpuAI 674 GAAGAC GAAGAC(2/6)
BsaAI 977 CACGTG [CT]AC^GT[GA]
BsaHI 181 GGCGTC G[GA]^CG[CT]C
BseAI 438 TCCGGA T^CCGGA
BseRI 176 GAGGAG GAGGAG(10/8)
Bsp1407I 4522 TGTACA T^GTACA
BspEI 438 TCCGGA T^CCGGA
BspLU11I 2170 ACATGT A^CATGT
BsrBI 54 CCGCTC CCGCTC(-3/-3)
BsrGI 4522 TGTACA T^GTACA
BstAPI 3100 GCACAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4291 TTCGAA TT^CGAA
Bsu36I 2684 CCTTAGG CC^T[ACGT]AGG
CelII 88 GCTCAGC GC^T[ACGT]AGC
CpoI 109 CGGTCCG CG^G[AT]CCG
Eco47III 609 AGCGCT AGC^GCT
Eco81I 2684 CCTTAGG CC^T[ACGT]AGG
Hin1I 181 GGCGTC G[GA]^CG[CT]C
HpaI 3315 GTTAAC GTT^AAC
MfeI 3336 CAATTG C^AATTG
MroI 438 TCCGGA T^CCGGA
MspA1I 1950 CAGCTG C[AC]G^C[GT]G
MunI 3336 CAATTG C^AATTG
NdeI 3196 CATATG CA^TATG
NspV 4291 TTCGAA TT^CGAA
PaeR7I 593 CTCGAG C^TCGAG
PmaCI 977 CACGTG CAC^GTG
PmlI 977 CACGTG CAC^GTG
PvuII 1950 CAGCTG CAG^CTG
RsrII 109 CGGTCCG CG^G[AT]CCG
SacI 1613 GAGCTC GAGCT^C
ScaI 2516 AGTACT AGT^ACT
SfuI 4291 TTCGAA TT^CGAA
SpeI 6192 ACTAGT A^CTAGT
SspBI 4522 TGTACA T^GTACA
XbaI 1786 TCTAGA T^CTAGA
XhoI 593 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 81 CGCG CG^CG
5117 CGCG
AhdI 1996 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3177 GACTAACTGTC
AseI 1876 ATTAAT AT^TAAT
6016 ATTAAT
AsnI 1876 ATTAAT AT^TAAT
6016 ATTAAT
AspEI 1996 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3177 GACTAACTGTC
AspHI 1613 GAGCTC G[AT]GC[AT]^C
1701 GTGCAC
AspI 4028 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5379 GACTCCGTC
BglI 2904 GCCTGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4988 GCCTCTTGGGC
BsiHKAI 1613 GAGCTC G[AT]GC[AT]^C
1701 GTGCAC
BspHI 1776 TCATGA T^CATGA
2254 TCATGA
BsrDI 696 CATTGC GCAATG(2/0)
2593 CATTGC
BssSI 4793 CACGAG CACGAG(-5/-1)
3938 CTCGTG
BstUI 81 CGCG CG^CG
5117 CGCG
Eam1105I 1996 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3177 GACTAACTGTC
HaeII 609 AGCGCT [GA]GCGC^[CT]
1295 AGCGCC
HgaI 4106 GACGC GACGC(5/10)
182 GCGTC
MvnI 81 CGCG CG^CG
5117 CGCG
PflFI 4028 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5379 GACTCCGTC
PshAI 774 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2477 GACAAGAGTC
PshBI 1876 ATTAAT AT^TAAT
6016 ATTAAT
RcaI 1776 TCATGA T^CATGA
2254 TCATGA
SapI 908 GCTCTTC GCTCTTC(1/4)
1615 GCTCTTC
StuI 30 AGGCCT AGG^CCT
5593 AGGCCT
Tth111I 4028 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
5379 GACTCCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AflII
AluI AlwI AlwNI ApoI Asp700I
AvaI AvaII BalI BamHI BanI
BanII BbvCI BbvI BcnI BfaI
BfrI BmyI BpmI BsaI BsaJI
BsaWI BsgI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRI EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI