################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII AccIII AcyI AgeI Alw44I Aor51HI ApaI ApaLI AscI AspI AviII BamHI BbeI BbrPI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoO65I FseI FspI HaeII HgaI Hin1I HpaI KasI KspI MluI MroI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmlI PshAI PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 2457 GGTACC G^GTACC AccI 6800 GTCTAC GT^[AC][GT]AC AccII 2170 CGCG CG^CG AclI 1401 AACGTT AA^CGTT AhdI 3361 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 4376 CCTCAGG CC^T[ACGT]AGG AseI 6242 ATTAAT AT^TAAT AsnI 6242 ATTAAT AT^TAAT Asp718I 2457 GGTACC G^GTACC AspEI 3361 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3448 CCTAGG C^CTAGG BcgI 1815 GCAGATGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 4404 GCCTGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 5559 AGATCT A^GATCT BlnI 3448 CCTAGG C^CTAGG BsaAI 908 CACGTA [CT]AC^GT[GA] BsaBI 5497 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspMI 4260 GCAGGT ACCTGC(4/8) BstUI 2170 CGCG CG^CG Bsu36I 4376 CCTCAGG CC^T[ACGT]AGG CfoI 2171 GCGC GCG^C Eam1105I 3361 GACAGTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 4376 CCTCAGG CC^T[ACGT]AGG FauI 426 GCGGG CCCGC(4/6) HapII 53 CCGG C^CGG HhaI 2171 GCGC GCG^C HinP1I 2171 GCGC G^CGC HpaII 53 CCGG C^CGG KpnI 2457 GGTACC GGTAC^C MamI 5497 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 53 CCGG C^CGG MvnI 2170 CGCG CG^CG NdeI 3835 CATATG CA^TATG PmeI 6322 GTTTAAAC GTTT^AAAC PshBI 6242 ATTAAT AT^TAAT Psp1406I 1401 AACGTT AA^CGTT SexAI 5691 ACCAGGT A^CC[AT]GGT SmaI 52 CCCGGG CCC^GGG StuI 2584 AGGCCT AGG^CCT XbaI 7898 TCTAGA T^CTAGA XmaCI 52 CCCGGG C^CCGGG XmaI 52 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflII 3780 CTTAAG C^TTAAG 6790 CTTAAG BalI 5799 TGGCCA TGG^CCA 7261 TGGCCA BbvCI 2461 CCTCAGC CCTCAGC(-5/-2) 4882 CCTCAGC BclI 5457 TGATCA T^GATCA 6832 TGATCA BcnI 52 CCCGG CC^[GC]GG 53 CCGGG BfrI 3780 CTTAAG C^TTAAG 6790 CTTAAG BsaI 1998 GAGACC GGTCTC(1/5) 5711 GAGACC BsgI 1060 CTGCAC GTGCAG(16/14) 1148 GTGCAG BspLU11I 810 ACATGT A^CATGT 6200 ACATGT DsaI 4593 CCATGG C^C[GA][CT]GG 7264 CCATGG EaeI 5799 TGGCCA [CT]^GGCC[GA] 7261 TGGCCA EcoNI 3711 CCTTTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 7508 CCTGTACCAGG EcoRV 217 GATATC GAT^ATC 4107 GATATC FbaI 5457 TGATCA T^GATCA 6832 TGATCA HincII 3279 GTTGAC GT[CT]^[GA]AC 4264 GTTGAC HindII 3279 GTTGAC GT[CT]^[GA]AC 4264 GTTGAC MluNI 5799 TGGCCA TGG^CCA 7261 TGGCCA MscI 5799 TGGCCA TGG^CCA 7261 TGGCCA NciI 52 CCCGG CC^[GC]GG 53 CCGGG NcoI 4593 CCATGG C^CATGG 7264 CCATGG PflMI 5400 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5659 CCAGCAATTGG PpuMI 899 AGGACCT [GA]G^G[AT]CC[CT] 3492 AGGTCCC PstI 641 CTGCAG CTGCA^G 6183 CTGCAG SpeI 945 ACTAGT A^CTAGT 5524 ACTAGT Van91I 5400 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5659 CCAGCAATTGG ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BciVI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MunI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI