##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccII AclI AflII AgeI
AocI Aor51HI ApaI AscI AviII
BbrPI BcgI BclI BfrI BsaAI
BsgI BsiEI BsiWI BsmBI BsmI
BspDI BspLU11I BspMI BssHII BssSI
BstBI BstUI Bsu36I ClaI CpoI
EagI EclXI Eco47III Eco52I Eco81I
EcoT22I FbaI FseI FspI HpaI
KspI MfeI MluI MunI MvnI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI Sse8387I
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 1302 GTATAC GT^[AC][GT]AC
AccIII 308 TCCGGA T^CCGGA
AflIII 533 ACACGT A^C[GA][CT]GT
AhdI 3377 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3917 GTGCAC G^TGCAC
ApaLI 3917 GTGCAC G^TGCAC
AspEI 3377 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2697 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2934 CCTAGG C^CTAGG
BbeI 799 GGCGCC GGCGC^C
BciVI 2276 GGATAC GTATCC(6/5)
BglII 3297 AGATCT A^GATCT
BlnI 2934 CCTAGG C^CTAGG
BsaBI 2013 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 308 TCCGGA [AT]^CCGG[AT]
BseAI 308 TCCGGA T^CCGGA
BspEI 308 TCCGGA T^CCGGA
BsrBI 1477 GAGCGG CCGCTC(-3/-3)
Bst1107I 1302 GTATAC GTA^TAC
BstAPI 981 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1302 GTATAC GTA^TAC
DrdI 415 GACTCCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3377 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3017 GAATTC G^AATTC
EcoRV 3337 GATATC GAT^ATC
HaeII 799 GGCGCC [GA]GCGC^[CT]
HgaI 2788 GACGC GACGC(5/10)
HincII 2811 GTCAAC GT[CT]^[GA]AC
HindII 2811 GTCAAC GT[CT]^[GA]AC
KasI 799 GGCGCC G^GCGCC
MamI 2013 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 308 TCCGGA T^CCGGA
NaeI 4250 GCCGGC GCC^GGC
NarI 799 GGCGCC GG^CGCC
NdeI 752 CATATG CA^TATG
NgoMIV 4250 GCCGGC G^CCGGC
PflFI 2697 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 790 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 580 GAAGAGC GCTCTTC(1/4)
SfoI 799 GGCGCC GGC^GCC
SmaI 2114 CCCGGG CCC^GGG
SphI 2119 GCATGC GCATG^C
StuI 3976 AGGCCT AGG^CCT
TaqI 2142 TCGA T^CGA
Tth111I 2697 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2142 TCGA T^CGA
Van91I 790 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2519 TCTAGA T^CTAGA
XmaCI 2114 CCCGGG C^CCGGG
XmaI 2114 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcyI 799 GGCGCC G[GA]^CG[CT]C
2788 GACGCC
Asp700I 2073 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3575 GAAACTTTTC
BamHI 741 GGATCC G^GATCC
2238 GGATCC
BbvCI 379 GCTGAGG CCTCAGC(-5/-2)
1488 GCTGAGG
BglI 3903 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4188 GCCTTCACGGC
BsaHI 799 GGCGCC G[GA]^CG[CT]C
2788 GACGCC
DraIII 2243 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4435 CACCGTGTG
EcoNI 342 CCTCGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1738 CCTCCCCAAGG
Hin1I 799 GGCGCC G[GA]^CG[CT]C
2788 GACGCC
HindIII 1702 AAGCTT A^AGCTT
2996 AAGCTT
MaeII 535 ACGT A^CGT
1575 ACGT
SmlI 227 CTCAAG C^T[CT][GA]AG
2407 CTCAAG
SpeI 4606 ACTAGT A^CTAGT
4746 ACTAGT
XmnI 2073 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3575 GAAACTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspHI BsrDI BsrFI
BsrGI BsrI BssKI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PshBI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI XhoII