##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AspEI BbeI BbrPI BglI BglII
BlpI Bpu1102I BsaAI BsiWI BspDI
BspLU11I BssSI BstAPI BstEII BstPI
Bsu36I CelII ClaI CpoI Eam1105I
Eco47III Eco81I EcoNI EcoO65I FseI
HincII HindII HpaI KasI MfeI
MluI MunI NarI NdeI NotI
NruI PacI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SalI SapI
SbfI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SwaI XcmI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccIII 1071 TCCGGA T^CCGGA
AcyI 178 GACGCC G[GA]^CG[CT]C
AflII 3982 CTTAAG C^TTAAG
AspI 4551 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1305 TGCGCA TGC^GCA
AvrII 4704 CCTAGG C^CTAGG
BbvCI 1331 CCTCAGC CCTCAGC(-5/-2)
BciVI 3326 GTATCC GTATCC(6/5)
BclI 4682 TGATCA T^GATCA
BfrI 3982 CTTAAG C^TTAAG
BlnI 4704 CCTAGG C^CTAGG
BsaBI 745 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 178 GACGCC G[GA]^CG[CT]C
BsaI 566 GAGACC GGTCTC(1/5)
BseAI 1071 TCCGGA T^CCGGA
BsiEI 126 CGGCCG CG[GA][CT]^CG
Bsp1407I 3062 TGTACA T^GTACA
BspEI 1071 TCCGGA T^CCGGA
BspMI 1272 ACCTGC ACCTGC(4/8)
BsrBI 123 GAGCGG CCGCTC(-3/-3)
BsrDI 1206 GCAATG GCAATG(2/0)
BsrGI 3062 TGTACA T^GTACA
BssHII 137 GCGCGC G^CGCGC
Bst1107I 3319 GTATAC GTA^TAC
BstBI 3632 TTCGAA TT^CGAA
BstXI 4476 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3319 GTATAC GTA^TAC
DraIII 1097 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3109 GACCTATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 126 CGGCCG C^GGCCG
EclXI 126 CGGCCG C^GGCCG
Eco52I 126 CGGCCG C^GGCCG
EcoRI 3232 GAATTC G^AATTC
EcoRV 640 GATATC GAT^ATC
FbaI 4682 TGATCA T^GATCA
FspI 1305 TGCGCA TGC^GCA
HaeII 1383 AGCGCC [GA]GCGC^[CT]
Hin1I 178 GACGCC G[GA]^CG[CT]C
KspI 38 CCGCGG CCGC^GG
MamI 745 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1071 TCCGGA T^CCGGA
NaeI 67 GCCGGC GCC^GGC
NgoMIV 67 GCCGGC G^CCGGC
NheI 233 GCTAGC G^CTAGC
NspV 3632 TTCGAA TT^CGAA
PaeR7I 528 CTCGAG C^TCGAG
PflFI 4551 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4719 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 379 GACGCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 584 CAGCTG CAG^CTG
SacII 38 CCGCGG CCGC^GG
SfuI 3632 TTCGAA TT^CGAA
SmaI 327 CCCGGG CCC^GGG
SspBI 3062 TGTACA T^GTACA
SspI 603 AATATT AAT^ATT
Tth111I 4551 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 528 CTCGAG C^TCGAG
XmaCI 327 CCCGGG C^CCGGG
XmaI 327 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Acc65I 2233 GGTACC G^GTACC
2290 GGTACC
AccI 1986 GTAGAC GT^[AC][GT]AC
3319 GTATAC
AgeI 1560 ACCGGT A^CCGGT
1656 ACCGGT
Asp700I 2892 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4196 GAACAGTTTC
Asp718I 2233 GGTACC G^GTACC
2290 GGTACC
BalI 1321 TGGCCA TGG^CCA
1924 TGGCCA
BspHI 2281 TCATGA T^CATGA
2653 TCATGA
DraI 1625 TTTAAA TTT^AAA
3209 TTTAAA
EcoT22I 2139 ATGCAT ATGCA^T
3626 ATGCAT
KpnI 2233 GGTACC GGTAC^C
2290 GGTACC
MluNI 1321 TGGCCA TGG^CCA
1924 TGGCCA
MscI 1321 TGGCCA TGG^CCA
1924 TGGCCA
NsiI 2139 ATGCAT ATGCA^T
3626 ATGCAT
NspI 281 ACATGC [GA]CATG^[CT]
1390 GCATGT
PinAI 1560 ACCGGT A^CCGGT
1656 ACCGGT
RcaI 2281 TCATGA T^CATGA
2653 TCATGA
ScaI 2732 AGTACT AGT^ACT
2746 AGTACT
XbaI 1108 TCTAGA T^CTAGA
3336 TCTAGA
XmnI 2892 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4196 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaI AvaII BamHI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PshBI PstI RsaI
SacI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII