##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AvaI BamHI BbeI BcgI BcnI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoT22I FseI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NarI NciI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
SspBI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 693 GACGCC G[GA]^CG[CT]C
Alw44I 3274 GTGCAC G^TGCAC
AlwNI 2037 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1154 GGGCCC GGGCC^C
ApaLI 3274 GTGCAC G^TGCAC
Asp700I 303 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 853 TGCGCA TGC^GCA
AvrII 2981 CCTAGG C^CTAGG
BalI 2825 TGGCCA TGG^CCA
BbvCI 2869 CCTCAGC CCTCAGC(-5/-2)
BclI 1236 TGATCA T^GATCA
BglI 1156 GCCCCTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2981 CCTAGG C^CTAGG
BsaBI 3754 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 693 GACGCC G[GA]^CG[CT]C
BseRI 1716 GAGGAG GAGGAG(10/8)
BsgI 604 GTGCAG GTGCAG(16/14)
BsrDI 3948 GCAATG GCAATG(2/0)
BstEII 3742 GGTGACC G^GT[ACGT]ACC
BstPI 3742 GGTGACC G^GT[ACGT]ACC
EaeI 2825 TGGCCA [CT]^GGCC[GA]
EcoO65I 3742 GGTGACC G^GT[ACGT]ACC
EcoRV 964 GATATC GAT^ATC
FauI 838 CCCGC CCCGC(4/6)
FbaI 1236 TGATCA T^GATCA
FspI 853 TGCGCA TGC^GCA
Hin1I 693 GACGCC G[GA]^CG[CT]C
HincII 2157 GTTAAC GT[CT]^[GA]AC
HindII 2157 GTTAAC GT[CT]^[GA]AC
HpaI 2157 GTTAAC GTT^AAC
MamI 3754 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2825 TGGCCA TGG^CCA
MscI 2825 TGGCCA TGG^CCA
NaeI 2019 GCCGGC GCC^GGC
NdeI 1438 CATATG CA^TATG
NgoMIV 2019 GCCGGC G^CCGGC
NspI 239 ACATGC [GA]CATG^[CT]
PflMI 1393 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1458 AGGACCT [GA]G^G[AT]CC[CT]
SapI 1552 GCTCTTC GCTCTTC(1/4)
SbfI 1500 CCTGCAGG CCTGCA^GG
Sse8387I 1500 CCTGCAGG CCTGCA^GG
StuI 2897 AGGCCT AGG^CCT
Van91I 1393 CCAATACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 794 CCAGCAGTCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 303 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 2118 CTTAAG C^TTAAG
3884 CTTAAG
AflIII 2473 ACACGT A^C[GA][CT]GT
3051 ACGTGT
AocI 251 CCTTAGG CC^T[ACGT]AGG
1159 CCTCAGG
AvaII 1199 GGTCC G^G[AT]CC
1459 GGACC
BanI 2763 GGTGCC G^G[CT][GA]CC
3357 GGCACC
BbrPI 802 CACGTG CAC^GTG
1387 CACGTG
BciVI 1433 GGATAC GTATCC(6/5)
790 GTATCC
BfrI 2118 CTTAAG C^TTAAG
3884 CTTAAG
BglII 3679 AGATCT A^GATCT
3753 AGATCT
BsaAI 802 CACGTG [CT]AC^GT[GA]
1387 CACGTG
BsrFI 786 GCCGGT [GA]^CCGG[CT]
2019 GCCGGC
BstXI 2744 CCAAGTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2879 CCAAAGTGCTGG
Bsu36I 251 CCTTAGG CC^T[ACGT]AGG
1159 CCTCAGG
Cfr10I 786 GCCGGT [GA]^CCGG[CT]
2019 GCCGGC
DraI 2545 TTTAAA TTT^AAA
3007 TTTAAA
Eco81I 251 CCTTAGG CC^T[ACGT]AGG
1159 CCTCAGG
HapII 787 CCGG C^CGG
2020 CCGG
HgaI 693 GACGC GACGC(5/10)
2359 GCGTC
HindIII 1327 AAGCTT A^AGCTT
3289 AAGCTT
HpaII 787 CCGG C^CGG
2020 CCGG
MspI 787 CCGG C^CGG
2020 CCGG
PmaCI 802 CACGTG CAC^GTG
1387 CACGTG
PmlI 802 CACGTG CAC^GTG
1387 CACGTG
PvuII 41 CAGCTG CAG^CTG
1126 CAGCTG
SacI 195 GAGCTC GAGCT^C
210 GAGCTC
ScaI 1920 AGTACT AGT^ACT
3823 AGTACT
SpeI 1748 ACTAGT A^CTAGT
1853 ACTAGT
SspI 2532 AATATT AAT^ATT
3205 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbsI BbvI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspMI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NcoI NdeII
NlaIII NlaIV PleI PstI RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII