##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AscI Asp718I AspI AviII AvrII
BbeI BcgI BglI BlnI BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI EcoT22I FseI
FspI HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
NarI NdeI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
Psp1406I PvuI RsrII SacII SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccII 1347 CGCG CG^CG
AcyI 875 GACGCC G[GA]^CG[CT]C
AflII 1734 CTTAAG C^TTAAG
AhdI 1930 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2020 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2854 AGCGCT AGC^GCT
ApaI 424 GGGCCC GGGCC^C
AspEI 1930 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 243 TGGCCA TGG^CCA
BamHI 3630 GGATCC G^GATCC
BanI 2216 GGCACC G^G[CT][GA]CC
BbvCI 1984 GCTGAGG CCTCAGC(-5/-2)
BciVI 690 GGATAC GTATCC(6/5)
BfrI 1734 CTTAAG C^TTAAG
BlpI 4353 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4353 GCTCAGC GC^T[ACGT]AGC
BsaHI 875 GACGCC G[GA]^CG[CT]C
BsmBI 324 CGTCTC CGTCTC(1/5)
BspMI 2342 GCAGGT ACCTGC(4/8)
BsrDI 3836 GCAATG GCAATG(2/0)
BstEII 1800 GGTCACC G^GT[ACGT]ACC
BstPI 1800 GGTCACC G^GT[ACGT]ACC
BstUI 1347 CGCG CG^CG
BstXI 3678 CCATGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2020 CCTCAGG CC^T[ACGT]AGG
CelII 4353 GCTCAGC GC^T[ACGT]AGC
CfoI 2855 GCGC GCG^C
DrdI 2198 GACACAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1391 CGGCCG C^GGCCG
Eam1105I 1930 GACTTTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1391 CGGCCG C^GGCCG
Eco47III 2854 AGCGCT AGC^GCT
Eco52I 1391 CGGCCG C^GGCCG
Eco81I 2020 CCTCAGG CC^T[ACGT]AGG
EcoNI 4099 CCTGAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1800 GGTCACC G^GT[ACGT]ACC
EcoRI 3862 GAATTC G^AATTC
EcoRV 1618 GATATC GAT^ATC
HaeII 2854 AGCGCT [GA]GCGC^[CT]
HgaI 875 GACGC GACGC(5/10)
HhaI 2855 GCGC GCG^C
Hin1I 875 GACGCC G[GA]^CG[CT]C
HinP1I 2855 GCGC G^CGC
MluNI 243 TGGCCA TGG^CCA
MscI 243 TGGCCA TGG^CCA
MvnI 1347 CGCG CG^CG
NaeI 256 GCCGGC GCC^GGC
NgoMIV 256 GCCGGC G^CCGGC
PaeR7I 2722 CTCGAG C^TCGAG
SacI 2590 GAGCTC GAGCT^C
SalI 585 GTCGAC G^TCGAC
ScaI 161 AGTACT AGT^ACT
SmaI 2813 CCCGGG CCC^GGG
XcmI 1476 CCACTATGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2722 CTCGAG C^TCGAG
XmaCI 2813 CCCGGG C^CCGGG
XmaI 2813 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 585 GTCGAC GT^[AC][GT]AC
3672 GTCTAC
AcsI 2736 GAATTT [GA]^AATT[CT]
3862 GAATTC
Alw44I 67 GTGCAC G^TGCAC
3767 GTGCAC
AlwNI 2918 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
3479 CAGCTACTG
ApaLI 67 GTGCAC G^TGCAC
3767 GTGCAC
ApoI 2736 GAATTT [GA]^AATT[CT]
3862 GAATTC
AseI 4378 ATTAAT AT^TAAT
4602 ATTAAT
AsnI 4378 ATTAAT AT^TAAT
4602 ATTAAT
Asp700I 1076 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4090 GAATGTTTTC
BbrPI 70 CACGTG CAC^GTG
4737 CACGTG
BclI 2661 TGATCA T^GATCA
3372 TGATCA
BglII 1609 AGATCT A^GATCT
2035 AGATCT
BsaI 2725 GAGACC GGTCTC(1/5)
3608 GAGACC
BsiEI 587 CGACCG CG[GA][CT]^CG
1391 CGGCCG
Bsp1407I 1262 TGTACA T^GTACA
2333 TGTACA
BspHI 563 TCATGA T^CATGA
1517 TCATGA
BsrFI 256 GCCGGC [GA]^CCGG[CT]
3763 GCCGGT
BsrGI 1262 TGTACA T^GTACA
2333 TGTACA
BstAPI 2307 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3760 GCAGCCGGTGC
Cfr10I 256 GCCGGC [GA]^CCGG[CT]
3763 GCCGGT
DraIII 1477 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
1553 CACCAAGTG
FbaI 2661 TGATCA T^GATCA
3372 TGATCA
HincII 585 GTCGAC GT[CT]^[GA]AC
3106 GTCAAC
HindII 585 GTCGAC GT[CT]^[GA]AC
3106 GTCAAC
HindIII 3589 AAGCTT A^AGCTT
3746 AAGCTT
NheI 433 GCTAGC G^CTAGC
4077 GCTAGC
PflMI 3184 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3652 CCACACCATGG
PmaCI 70 CACGTG CAC^GTG
4737 CACGTG
PmlI 70 CACGTG CAC^GTG
4737 CACGTG
PshAI 645 GACATTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3563 GACAACTGTC
PshBI 4378 ATTAAT AT^TAAT
4602 ATTAAT
PstI 1523 CTGCAG CTGCA^G
2386 CTGCAG
PvuII 1982 CAGCTG CAG^CTG
2411 CAGCTG
RcaI 563 TCATGA T^CATGA
1517 TCATGA
SspBI 1262 TGTACA T^GTACA
2333 TGTACA
SspI 2490 AATATT AAT^ATT
2993 AATATT
StuI 1179 AGGCCT AGG^CCT
2642 AGGCCT
Van91I 3184 CCAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3652 CCACACCATGG
XmnI 1076 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4090 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII