##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI Asp700I Asp718I AviII AvrII
BbeI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BseAI BsiWI BsmBI BspEI BspLU11I
BssHII BstBI BstEII BstPI BstXI
CelII CpoI DrdI Eco47III EcoO65I
EcoRV EcoT22I FbaI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI MamI MfeI MluI MroI
MunI NarI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI RsrII SalI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AclI 2222 AACGTT AA^CGTT
AhdI 1411 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1474 CCTCAGG CC^T[ACGT]AGG
AseI 3781 ATTAAT AT^TAAT
AsnI 3781 ATTAAT AT^TAAT
AspEI 1411 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 589 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2695 TGGCCA TGG^CCA
BamHI 1086 GGATCC G^GATCC
BbrPI 1386 CACGTG CAC^GTG
BbvCI 3018 CCTCAGC CCTCAGC(-5/-2)
BcgI 1488 CGACCGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2209 GCCCCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1386 CACGTG [CT]AC^GT[GA]
BsaWI 1787 ACCGGA [AT]^CCGG[AT]
Bsp1407I 3229 TGTACA T^GTACA
BspDI 1246 ATCGAT AT^CGAT
BspHI 1405 TCATGA T^CATGA
BsrBI 632 CCGCTC CCGCTC(-3/-3)
BsrFI 3026 GCCGGC [GA]^CCGG[CT]
BsrGI 3229 TGTACA T^GTACA
BssSI 3485 CTCGTG CACGAG(-5/-1)
BstAPI 2757 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1474 CCTCAGG CC^T[ACGT]AGG
Cfr10I 3026 GCCGGC [GA]^CCGG[CT]
ClaI 1246 ATCGAT AT^CGAT
EagI 3024 CGGCCG C^GGCCG
Eam1105I 1411 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3024 CGGCCG C^GGCCG
Eco52I 3024 CGGCCG C^GGCCG
Eco81I 1474 CCTCAGG CC^T[ACGT]AGG
EcoRI 2348 GAATTC G^AATTC
FseI 3025 GGCCGGCC GGCCGG^CC
KspI 759 CCGCGG CCGC^GG
MluNI 2695 TGGCCA TGG^CCA
MscI 2695 TGGCCA TGG^CCA
NaeI 3026 GCCGGC GCC^GGC
NdeI 1239 CATATG CA^TATG
NgoMIV 3026 GCCGGC G^CCGGC
PflFI 589 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1386 CACGTG CAC^GTG
PmlI 1386 CACGTG CAC^GTG
PshBI 3781 ATTAAT AT^TAAT
Psp1406I 2222 AACGTT AA^CGTT
PvuI 1248 CGATCG CGAT^CG
RcaI 1405 TCATGA T^CATGA
SacII 759 CCGCGG CCGC^GG
SapI 1572 GCTCTTC GCTCTTC(1/4)
SbfI 2381 CCTGCAGG CCTGCA^GG
SexAI 1859 ACCAGGT A^CC[AT]GGT
SmaI 939 CCCGGG CCC^GGG
Sse8387I 2381 CCTGCAGG CCTGCA^GG
SspBI 3229 TGTACA T^GTACA
Tth111I 589 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 245 TCTAGA T^CTAGA
XmaCI 939 CCCGGG C^CCGGG
XmaI 939 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaI 939 CCCGGG C^[CT]CG[GA]G
1399 CCCGAG
BbsI 416 GAAGAC GAAGAC(2/6)
3405 GAAGAC
BpuAI 416 GAAGAC GAAGAC(2/6)
3405 GAAGAC
BsoBI 939 CCCGGG C^[CT]CG[GA]G
1399 CCCGAG
Bst1107I 464 GTATAC GTA^TAC
3696 GTATAC
BstZ17I 464 GTATAC GTA^TAC
3696 GTATAC
CfoI 714 GCGC GCG^C
2116 GCGC
DraIII 1858 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2982 CACAGTGTG
HaeII 713 AGCGCC [GA]GCGC^[CT]
2115 GGCGCT
HgaI 959 GCGTC GACGC(5/10)
1648 GCGTC
HhaI 714 GCGC GCG^C
2116 GCGC
HinP1I 714 GCGC G^CGC
2116 GCGC
MslI 227 CACTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
807 CATTGTGGTG
NspI 1132 GCATGT [GA]CATG^[CT]
1483 ACATGC
PvuII 2353 CAGCTG CAG^CTG
3588 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII