##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I AspI AviII AvrII
BclI BfrI BlnI BsaBI BsaWI
BseAI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstZ17I ClaI CpoI
DraI EagI EclXI Eco52I EcoRV
EcoT22I FbaI FspI HpaI MamI
MfeI MluI MroI MunI NdeI
NotI NsiI PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SgrAI SnaBI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 154 GTCTAC GT^[AC][GT]AC
AhdI 2270 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2270 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 639 TGGCCA TGG^CCA
BamHI 3212 GGATCC G^GATCC
BbeI 4189 GGCGCC GGCGC^C
BbrPI 3245 CACGTG CAC^GTG
BciVI 5687 GTATCC GTATCC(6/5)
BglII 2323 AGATCT A^GATCT
BsaAI 3245 CACGTG [CT]AC^GT[GA]
BsiEI 682 CGACCG CG[GA][CT]^CG
BsmBI 5405 GAGACG CGTCTC(1/5)
BssSI 1588 CACGAG CACGAG(-5/-1)
BstAPI 4610 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4108 GGTGACC G^GT[ACGT]ACC
BstPI 4108 GGTGACC G^GT[ACGT]ACC
Eam1105I 2270 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4108 GGTGACC G^GT[ACGT]ACC
EcoRI 2741 GAATTC G^AATTC
FseI 3194 GGCCGGCC GGCCGG^CC
KasI 4189 GGCGCC G^GCGCC
KspI 5190 CCGCGG CCGC^GG
MluNI 639 TGGCCA TGG^CCA
MscI 639 TGGCCA TGG^CCA
NaeI 3195 GCCGGC GCC^GGC
NarI 4189 GGCGCC GG^CGCC
NgoMIV 3195 GCCGGC G^CCGGC
NheI 3302 GCTAGC G^CTAGC
NruI 728 TCGCGA TCG^CGA
PmaCI 3245 CACGTG CAC^GTG
PmlI 3245 CACGTG CAC^GTG
SacII 5190 CCGCGG CCGC^GG
SfiI 4574 GGCCTTGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4189 GGCGCC GGC^GCC
SpeI 5544 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 3056 GACGCC G[GA]^CG[CT]C
4189 GGCGCC
Aor51HI 606 AGCGCT AGC^GCT
3008 AGCGCT
BcgI 917 GCAGCCAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4940 GCAGCAGACTCG
BsaHI 3056 GACGCC G[GA]^CG[CT]C
4189 GGCGCC
BsrBI 117 CCGCTC CCGCTC(-3/-3)
54 GAGCGG
BsrFI 3195 GCCGGC [GA]^CCGG[CT]
3761 ACCGGC
BstBI 1571 TTCGAA TT^CGAA
5203 TTCGAA
Cfr10I 3195 GCCGGC [GA]^CCGG[CT]
3761 ACCGGC
DraIII 2223 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3584 CACTTTGTG
DrdI 3916 GACTGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5044 GACACGGATGTC
Eco47III 606 AGCGCT AGC^GCT
3008 AGCGCT
Hin1I 3056 GACGCC G[GA]^CG[CT]C
4189 GGCGCC
HincII 1824 GTCAAC GT[CT]^[GA]AC
5078 GTCAAC
HindII 1824 GTCAAC GT[CT]^[GA]AC
5078 GTCAAC
HindIII 1116 AAGCTT A^AGCTT
3070 AAGCTT
NcoI 177 CCATGG C^CATGG
2887 CCATGG
NspV 1571 TTCGAA TT^CGAA
5203 TTCGAA
PshAI 1938 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2441 GACCCAGGTC
SacI 3091 GAGCTC GAGCT^C
4205 GAGCTC
SfuI 1571 TTCGAA TT^CGAA
5203 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
Acc65I AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI Asp718I AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI