##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
AscI AseI AsnI Asp718I AspEI
AspI BamHI BbrPI BcgI BciVI
BclI BfrI BglII BsaAI BsaBI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI DraI Eam1105I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI KpnI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI SalI SfiI SfuI SnaBI
SpeI SspBI SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3180 GTAGAC GT^[AC][GT]AC
AcsI 4875 AAATTC [GA]^AATT[CT]
AflIII 4276 ACACGT A^C[GA][CT]GT
AgeI 4861 ACCGGT A^CCGGT
Alw44I 1974 GTGCAC G^TGCAC
ApaLI 1974 GTGCAC G^TGCAC
ApoI 4875 AAATTC [GA]^AATT[CT]
Asp700I 917 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 4258 TGCGCA TGC^GCA
AvrII 4681 CCTAGG C^CTAGG
BlnI 4681 CCTAGG C^CTAGG
BsiEI 1930 CGGCCG CG[GA][CT]^CG
BsmBI 3971 CGTCTC CGTCTC(1/5)
BspHI 3367 TCATGA T^CATGA
BsrDI 4147 CATTGC GCAATG(2/0)
CpoI 128 CGGTCCG CG^G[AT]CCG
DrdI 3183 GACTTCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1930 CGGCCG C^GGCCG
EclXI 1930 CGGCCG C^GGCCG
Eco52I 1930 CGGCCG C^GGCCG
FspI 4258 TGCGCA TGC^GCA
HincII 3192 GTCAAC GT[CT]^[GA]AC
HindII 3192 GTCAAC GT[CT]^[GA]AC
MaeII 4278 ACGT A^CGT
NaeI 1928 GCCGGC GCC^GGC
NgoMIV 1928 GCCGGC G^CCGGC
PinAI 4861 ACCGGT A^CCGGT
PshAI 3535 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3367 TCATGA T^CATGA
RsrII 128 CGGTCCG CG^G[AT]CCG
SacI 815 GAGCTC GAGCT^C
SbfI 2573 CCTGCAGG CCTGCA^GG
ScaI 3662 AGTACT AGT^ACT
SgrAI 2109 CACCGGCG C[GA]^CCGG[CT]G
SphI 998 GCATGC GCATG^C
Sse8387I 2573 CCTGCAGG CCTGCA^GG
XmnI 917 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BalI 594 TGGCCA TGG^CCA
1459 TGGCCA
BbsI 2307 GTCTTC GAAGAC(2/6)
3762 GTCTTC
BpuAI 2307 GTCTTC GAAGAC(2/6)
3762 GTCTTC
BsrBI 1040 CCGCTC CCGCTC(-3/-3)
1139 CCGCTC
BssSI 1889 CACGAG CACGAG(-5/-1)
2022 CACGAG
KspI 47 CCGCGG CCGC^GG
2120 CCGCGG
MluNI 594 TGGCCA TGG^CCA
1459 TGGCCA
MscI 594 TGGCCA TGG^CCA
1459 TGGCCA
MseI 4430 TTAA T^TAA
5003 TTAA
NcoI 2780 CCATGG C^CATGG
3483 CCATGG
NspI 998 GCATGC [GA]CATG^[CT]
2398 ACATGC
PaeR7I 812 CTCGAG C^TCGAG
3120 CTCGAG
SacII 47 CCGCGG CCGC^GG
2120 CCGCGG
SexAI 1375 ACCAGGT A^CC[AT]GGT
1684 ACCAGGT
StuI 834 AGGCCT AGG^CCT
2912 AGGCCT
Tru9I 4430 TTAA T^TAA
5003 TTAA
XbaI 2942 TCTAGA T^CTAGA
4576 TCTAGA
XhoI 812 CTCGAG C^TCGAG
3120 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 133 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI AlwI AlwNI AocI Aor51HI
ApaI AspHI AvaI AvaII BanI
BanII BbeI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrFI BsrI BssHII
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MroI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI